| Project/Area Number |
14207023
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Fujita Health University |
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Principal Investigator |
ISHII Akira Fujita Health University, School of Medicine, Professor, 医学部, 教授 (30252175)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAOKA Kenzo Yamanashi University, Faculty of Engineering, Professor, 工学部, 教授 (80107218)
SUZUKI Osamu Hamamatsu University, School of Medicine, Professor, 医学部, 教授 (70093044)
WATANABE Kanako Hamamatsu University, School of Medicine, Assistant Professor, 医学部, 助教授 (70288546)
KATSUMATA Yoshinao Nagoya University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (30109326)
KANEKO Rina Fujita Health University, School of Medicine, Research Associate, 医学部, 助手 (70367697)
服部 秀樹 愛知医科大学, 医学部, 助教授 (30107817)
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| Project Period (FY) |
2002 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥42,250,000 (Direct Cost: ¥32,500,000、Indirect Cost: ¥9,750,000)
Fiscal Year 2005: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2004: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2003: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2002: ¥31,720,000 (Direct Cost: ¥24,400,000、Indirect Cost: ¥7,320,000)
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| Keywords | High-performanced liquid chromatography (HPLC) / Electrospray ionization (ESI) / Laser spray ionization (LSI) / Time-of-flight mass spectrometer (TOF-MS) / Aconitine / Positive ion mode / Negative ion mode / Drugs of abuse / アマニチン |
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| Research Abstract |
Mass spectrometry (MS) is a useful tool for detecting and identifying drugs and poisons ; recent progress of interfaces has enabled high-performance liquid chromatography (LC) to couple as LC/MS or LC/MS/MS. Electrospray ionization (ESI) is widely used as an ionization method for LC/MS, but softer ionization methods with higher efficiency are needed. In this project, we have tried to apply laser ionization (LSI), a new soft ionization method to forensic toxicological analyses. In LSI, target compounds are ionized by infrared laser beam.
In this project, one of the most difficult problem is the appropriate adjustment of laser beam onto the tip of the capillary ; the elongation of light path and the monitoring of the capillary using a CCD camera, were useful for fee adjustment of the laser focus on the capillary tip.
We used a time-of-flight (TOF) MS as a LC/MS system, and TOF-MS has higher mass resolution ; we have developed a sensitive and specific method for identifying and quantitating Aconitum alkaloids in human body fluids and Aconitum tubers, which will be published in the Journal of Mass Spectrometry.
By LSI, some drugs-of-abuse, e.g.cocaine, morphines and amphetamines were ionized with 5 to 10 times higher intensities, compared to those by the conventional ESI. In the negative mode, propionic acid derivative analgesic-antipyretic compounds were ionized with 20 to 30 times higher intensities, compared to those by ESI. All those compounds were applied direct infusion mode. However, the ion intensities were quite unstable ; thus it seems difficult for the on-line analysis of target compounds.
In the future project, this LSI ionization should be applied to a nanoscale LC system to obtain more stable ionization.
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