| Project/Area Number |
14208089
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Tohoku University |
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Principal Investigator |
IDE Hiroyuki Tohoku University, Graduate School of Life Sciences, Professor, 大学院・生命科学研究科, 教授 (70022704)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Koji Tohoku University, Graduate School of Life Sciences, Associate Professor, 大学院・生命科学研究科, 助教授 (70261550)
松田 大樹 日本学術振興会, 特別研究員 (60801363)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2004: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2003: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2002: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
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| Keywords | Xenopus / limb regeneration / bifurcation, segmentation / chondrogenesis / BMP / SHH / 分岐 / 分節 / 筋肉組織 / 四肢の再生 / 分岐と分節 / パターン形成 / 四肢 / 再生 / FGF-10 |
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| Research Abstract |
Frog limbs show important characteristics in regeneration potency between newt with complete regeneration potency and mammals with no rgeneration potency. Froglets of Xenopus lexis rgenerate cartilage rod, spike, after amputaion of limbs. The spike was found to be an incomplete regenerate without bifurcation and segmentation. Furthermore, the spike has no muscle tissues.
To induce bifurcation in the spike, we examined the expression of BMP. No expression of BMP gene in the spike was observed, and implantation of beads contraining BMP protein into the spike induced the segment formation in the cartilage. THe induction of segmentation was completely blocked by the addition of chordin, a BMP inhibitor. These results suggest that BMP play a role in the segmentation of regenerates.
The frequency of spike formation with bifurcation increased by the implantation of SHH protein.
To induce muscke formation in the spike, Muscle precursor cells were transplanted into the spike. Differentiation of muscle tissue was observed in the spike by the cells producing hepatocyte growth factor, an attractant of myogenic cells. These results suggest that the lack of muscle tissues in the spike can be restored at least partially.
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