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Bifurcation and segmentation in regenerating limb

Research Project

Project/Area Number 14208089
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionTohoku University

Principal Investigator

IDE Hiroyuki  Tohoku University, Graduate School of Life Sciences, Professor, 大学院・生命科学研究科, 教授 (70022704)

Co-Investigator(Kenkyū-buntansha) TAMURA Koji  Tohoku University, Graduate School of Life Sciences, Associate Professor, 大学院・生命科学研究科, 助教授 (70261550)
松田 大樹  日本学術振興会, 特別研究員 (60801363)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2004: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2003: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2002: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
KeywordsXenopus / limb regeneration / bifurcation, segmentation / chondrogenesis / BMP / SHH / 分岐 / 分節 / 筋肉組織 / 四肢の再生 / 分岐と分節 / パターン形成 / 四肢 / 再生 / FGF-10
Research Abstract

Frog limbs show important characteristics in regeneration potency between newt with complete regeneration potency and mammals with no rgeneration potency. Froglets of Xenopus lexis rgenerate cartilage rod, spike, after amputaion of limbs. The spike was found to be an incomplete regenerate without bifurcation and segmentation. Furthermore, the spike has no muscle tissues.
To induce bifurcation in the spike, we examined the expression of BMP. No expression of BMP gene in the spike was observed, and implantation of beads contraining BMP protein into the spike induced the segment formation in the cartilage. THe induction of segmentation was completely blocked by the addition of chordin, a BMP inhibitor. These results suggest that BMP play a role in the segmentation of regenerates.
The frequency of spike formation with bifurcation increased by the implantation of SHH protein.
To induce muscke formation in the spike, Muscle precursor cells were transplanted into the spike. Differentiation of muscle tissue was observed in the spike by the cells producing hepatocyte growth factor, an attractant of myogenic cells. These results suggest that the lack of muscle tissues in the spike can be restored at least partially.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (17 results)

All 2005 2003 2002 Other

All Journal Article (10 results) Book (2 results) Publications (5 results)

  • [Journal Article] Joint development in Xenopus laevis and induction of segmentation in regenerating froglet limb (spike)2005

    • Author(s)
      Satoh, A., Amano, K., Tamura, K., Ide, H.
    • Journal Title

      Developmental Dynamics (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Muscle formation in regenerating Xenopus limb2005

    • Author(s)
      Satoh, A., Ide, H., Tamura, K.
    • Journal Title

      Developmental Dynamics (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Joint development in Xenopus laevis and induction of segmentation in regenerating froglet limb (spike)2005

    • Author(s)
      Satoh, A., Amano, K., Tamura, K., Ide, H.
    • Journal Title

      Developmental Dynamic (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Muscle formation in regenerating Xenopus limb.2005

    • Author(s)
      Satoh, A., Ide, H., Tamura, K.
    • Journal Title

      Developmental Dynamic (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Muscle formation in regenerating Xenopus froglet limb2005

    • Author(s)
      Sato, A
    • Journal Title

      Developmental Dynamics (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular characterization of radical spoke subcomplex protein3 and heat shook protein 40 in sperm flagella of the ascidian2005

    • Author(s)
      Satouh, H.
    • Journal Title

      Molecular Biology of Cell 16

      Pages: 626-636

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Epherin-A2 regulates position-specific cell affinity and is involved in cartilage morphogenesis in the chick limb bud2003

    • Author(s)
      Wada, N., Tanaka, H., Ide, H., Nohno, T.
    • Journal Title

      Developmental Biology 264

      Pages: 550-563

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epherin-A2 regulates position-specific cell affinity and is involved in cartilage morphogenesis in the chick limb bud.2003

    • Author(s)
      Wada, N., Tanaka, H., Ide, H., Nohno, T.
    • Journal Title

      Developmental Biology 264

      Pages: 550-563

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Anteroposterior axis formation in Xenopus Limb bud recombinants : A model of pattern formation during limb regeneration2002

    • Author(s)
      Yokoyama, H., Tamura, K., Ide, H.
    • Journal Title

      Developmental Dynamics 225

      Pages: 277-288

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Anteroposterior axis formation in Xenopus limb bud recombinants : A model of pattern formation during limb regeneration2002

    • Author(s)
      Yokoyama H., Tamura, K., Ide, H.
    • Journal Title

      Developmental Dynamic 225

      Pages: 277-288

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 四肢の形成機構2003

    • Author(s)
      井出宏之(編著)
    • Total Pages
      352
    • Publisher
      アイピーシー
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Shishinokeiseikikou2003

    • Author(s)
      Ide, H.
    • Total Pages
      352
    • Publisher
      IPC
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Wada, N.: "Epherin-A2 regulates position-specific cell affinity and is involved in cartilage morphogenesis in the chick limb bud"Developmental Biology. 264. 550-563 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tamura, K.: "Expression of rigf, a member of VEGF family, correlates with vascular patterning in the developing chick limb bud"Mechanisms of Development. 120. 199-209 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 井出宏之(編著): "四肢の形成機構"(株)アイピーシー. 341 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Yokoyama: "Anteroposterior axis formation in Xenopus limb bud recombinants"Developmental Dynamics. 225. 277-288 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] D.Saito: "Specification and determination of limb identity : evidence for inhibitory regulation of Tbx gene expression"Development. 129. 211-220 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2018-06-11  

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