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Regionalization of developing brain and neuronal migration

Research Project

Project/Area Number 14208095
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionOsaka University

Principal Investigator

MURAKAMI Fujio  Osaka University, Graduate School of Frontier Biosciences, Professor, 大学院・生命機能研究科, 教授 (20089882)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Nobuhiko  Osaka University, Graduate School of Frontier Biosciences, Professor, 大学院・生命機能研究科, 教授 (00191429)
TAMADA Atsushi  RIKEN, Brain Science Institute, Researcher, 脳科学総合研究センター, 研究員 (60270576)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥53,170,000 (Direct Cost: ¥40,900,000、Indirect Cost: ¥12,270,000)
Fiscal Year 2004: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2003: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2002: ¥31,330,000 (Direct Cost: ¥24,100,000、Indirect Cost: ¥7,230,000)
Keywordsrat / embryo / electroporation / cell migration / area-specificity / GFP / organ culture / cortical pyramidal cell / 胎仔 / AD67-GFPノックインマウス / GABA作動性ニューロン / タイムラプス / 菱脳唇 / 神経節隆起
Research Abstract

Neuronal migration is crucial for the construction of neuronal architecture such as layers and nuclei. The present study carried out a systematic analysis to examine whether the migratory behavior of cortical neurons derived from the cortical VZ is stage-dependent. We labeled VZ cells of mouse embryos with green fluorescent protein(gfp)-encoding plasmids by in utero electroporation and evaluated the labeled cells after appropriate survival periods. After electroporation at either embryonic day (E) 12.5 or E15.5, GFP+VZ cells were initially spindle-shaped and radially oriented. After leaving the VZ, they transformed into round or horizontally oriented fusiform neurons with many short processes. They then seemed to gradually change into radially oriented bipolar cells as they moved upward. Whereas the earliest emigrants from the VZ labeled at E12.5(early-born neurons) reached the top of the cortical plate(CP) after these changes, VZ cells labeled at E15.5(late-born neurons) further migrated along the length of radial fibers to reach the top of the CP. A dominant negative form of the gene for cyclin-dependent kinase 5(Cdk5DN) was then introduced into VZ cells. Transfection of E12.5 VZ with cdk5dn did not disrupt the migration of the early-born neurons. However, this caused a failure in migration of the late-born neurons, although they transformed into bipolar shapes in the intermediate zone. Thus, there appear to be at least two distinct migratory phases of cortical neurons : one common to the early- and late-born neurons, and the other specific to late-born neurons and Cdk5-dependent.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (42 results)

All 2004 2003 2002 Other

All Journal Article (28 results) Publications (14 results)

  • [Journal Article] The Divergent Robo Family Protein Rig-1/Robo3 Is a Negative Regulator of Slit Responsiveness Required for Midline Crossing by Commissural Axons2004

    • Author(s)
      Christelle Sabatier
    • Journal Title

      Cell 117

      Pages: 157-169

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distinct Migratory Behavior of Early- and Late-born Neurons in the Cerebral Cortex2004

    • Author(s)
      Hatanaka, Y
    • Journal Title

      The Journal of Comparative Neurology 479

      Pages: 1-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The Divergent Robo Family Protein Rig-1/Robo3 Is a Negative Regulator of Slit Responsiveness Required for Midline Crossing by Commissural Axons.2004

    • Author(s)
      Christelle Sabatier, Andrew S.Plump, Le Ma, Katja Brose, Atsushi Tamada, Fujio Murakami, Eva Y-H.P.Lee, Marc Tessier-Lavigne
    • Journal Title

      Cell 117

      Pages: 157-169

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Conserved Roles for Slit and Robo Proteins in Midline Commissural Axon Guidance.2004

    • Author(s)
      Hua Long, Christelle Sabatier, Le Ma, Andrew Plump, Wenlin Yuan, David Ornitz, Atsushi Tamada, Fujio Murakami, Corey S.Goodman, Marc Tessier-Lavigne
    • Journal Title

      Neuron 42

      Pages: 213-223

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] BDNF and NT-3 promote thalamocortical axon growth with distinct substrate and temporal dependency.2004

    • Author(s)
      Kenji Hanamura Akiko Harada, Ritsuko Katoh-Semba, Fujio Murakami, Nobuhiko Yamamoto
    • Journal Title

      Eur J.Neurosci 19

      Pages: 1485-1493

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of the Genes that are Expressed in the Upper Layers of the Neocortex.2004

    • Author(s)
      Yun Zhong, Makoto Takemoto, Tsuyoshi Fukuda, Yuki Hattori, Fujio Murakami, Daisuke Nakajima, Manabu Nakayama, Nobuhiko Yamamoto
    • Journal Title

      Cerebral Cortex 14

      Pages: 1144-1152

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Contacts between the commissural axons and the floor plate cells are mediated by nectins.2004

    • Author(s)
      Okabe, N., Shimizu, K., Ozaki-Kuroda, K., Nakanishi, H., Morimoto, K., Takeuchi, M., Katsumaru, H., Murakami, F., Takai, Y.
    • Journal Title

      Dev.Biol. 273

      Pages: 244-256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distinct Migratory Behavior of Early- and Late-born Neurons Derived from the Cortical Ventricular Zone.2004

    • Author(s)
      Yumiko Hatanaka, Shin-ichi Hisanaga, Claus W.Heizmann, Fujio Murakami
    • Journal Title

      J.Comp Neurol 479

      Pages: 1-14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The Divergent Robo Family Protein Rig-1/Robo3 Is a Negative Regulator of Slit Responsiveness Required for Midline Crossing by Commissural Axons.2004

    • Author(s)
      Christelle Sabatier
    • Journal Title

      Cell 117

      Pages: 157-169

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Conserved Roles for Slit and Robo Proteins in Midline Commissural Axon Guidance.2004

    • Author(s)
      Hua Long
    • Journal Title

      Neuron 42

      Pages: 213-223

    • Related Report
      2004 Annual Research Report
  • [Journal Article] BDNF and NT-3 promote thalamocortical axon growth with distinct substrate and temporal dependency.2004

    • Author(s)
      Kenji Hanamura
    • Journal Title

      Eur J.Neurosci 19

      Pages: 1485-1493

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Identification of the Genes that are Expressed in the Upper Layers of the Neocortex.2004

    • Author(s)
      Yun Zhong
    • Journal Title

      Cerebral Cortex 14

      Pages: 1144-1152

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Contacts between the commissural axons and the floor plate cells are mediated by nectins.2004

    • Author(s)
      Noriko Okabe
    • Journal Title

      Dev.Biol. 273

      Pages: 244-256

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Distinct Migratory Behavior of Early- and Late-born Neurons Derived from the Cortical Ventricular Zone.2004

    • Author(s)
      Yumiko Hatanaka
    • Journal Title

      J.Comp Neurol. 479

      Pages: 1-14

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Pathfinding and growth termination of primary trigeminal sensory afferents in the embryonic rat hindbrain2003

    • Author(s)
      Miyahara, M
    • Journal Title

      The Journal of Comparative Neurology 460

      Pages: 503-513

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Multimodal tangential migration of neocortical GABAergic neurons independent of GPI-anchored proteins2003

    • Author(s)
      Tanaka, D
    • Journal Title

      Development 130

      Pages: 5803-5813

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pathfinding and growth termination of primary trigeminal sensory afferents in the embryonic rat hindbrain.2003

    • Author(s)
      Miyahara, M, Shirasaki, R, Tashiro, Y, Muguruma, K, Heizmann, CW, Murakami, F.
    • Journal Title

      J.Comp Neurol. 460

      Pages: 503-513

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Rundown of a transient potassium current is attributable to changes in channel voltage dependence.2003

    • Author(s)
      Hattori, S., Murakami, F., Song, W.-J.
    • Journal Title

      Synapse 48

      Pages: 57-65

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] MuSC is involved in regulating axonal fasciculation of mouse primary.2003

    • Author(s)
      Kawauchi, D., Kobayashi, H., Sekine-Aizawa, Y., Fujita, S.C., Murakami, F.
    • Journal Title

      Eur J Neurosci. 18

      Pages: 2244-2252

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Multimodal tangential migration of neocortical GABAergic neurons independent of GPI-anchored proteins.2003

    • Author(s)
      Tanaka, D., Nakaya, Y., Yanagawa, Y., Obata, K., Murakami, F.
    • Journal Title

      Development 130

      Pages: 5803-5813

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Quantitative relationship between Kv4.2 mRNA and A-Type K+ current in rat striatal cholinergic interneurons during development.2003

    • Author(s)
      Hattori, S., Murakami, F., Song, W.-J.
    • Journal Title

      J.Neurophysiol. 90

      Pages: 175-183

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro analysis of the origin, migratory behavior and maturation of cortical pyramidal cells2002

    • Author(s)
      Hatanaka, Y
    • Journal Title

      The Journal of Comparative Neurology 454

      Pages: 1-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Crossing the ventral midline causes neurons to change their response to floor plate and alar plate attractive cues during transmedian migration2002

    • Author(s)
      Taniguchi, H
    • Journal Title

      Developmental Biology 249

      Pages: 321-332

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro analysis of the origin, migratory behavior and maturation of cortical pyramidal cells.2002

    • Author(s)
      Hatanaka, Y., Murakami, F.
    • Journal Title

      J.Comp.Neurol. 454

      Pages: 1-14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Filamin A-interacting protein FILIP regulates cortical cell migration out of the ventricular zone.2002

    • Author(s)
      Nagano, T., Yoneda, T., Hatanaka, Y., Kubota, C., Murakami, F, Sato, M.
    • Journal Title

      Nature Cell Biology 4

      Pages: 495-501

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Crossing the ventral midline causes neurons to change their response to floor plate and alar plate attractive cues during transmedian migration.2002

    • Author(s)
      Taniguchi, H., Tamada, A., Kennedy, T.E., Murakami, F.
    • Journal Title

      Dev.Biol. 249

      Pages: 321-332

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ephrin-B3-EphA4 interactions regulate the growth of specific thalamocortical axon populations in vitro.2002

    • Author(s)
      Takemoto, M., Fukuda, T., Sonoda, R., Murakami, F., Tanaka, H., Yamamoto, N.
    • Journal Title

      Eur.J.Neurosci 16

      Pages: 1168-1172

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Generation of neural crest-derived peripheral neurons and floor plate cells from mouse and primate embryonic stem cells.2002

    • Author(s)
      Mizuseki, K., Sakamoto, T., Watanabe, K., Muguruma, K., Ikeya, M., Nishiyama, A., Arakawa, A., Suemori, H., Nakatsuji, N., Kawasaki, H., Murakami, F., Sasai, Y.
    • Journal Title

      PNAS 100

      Pages: 5828-5833

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Mizuseki, K.: "Generation of neural crest-derived peripheral neurons and floor plate cells from mouse and primate embryonic stem cells"PNAS. 100. 5828-5833 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyahara, M.: "Pathfinding and growth termination of primary trigeminal sensory afferents in the embryonic rat hindbrain"J.Comp.Neurol.. 460. 503-513 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hattori, S.: "Rundown of a transient potassium current is involved is attributable to changes in channel voltage dependence"Synapse. 48. 57-65 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawauchi, D.: "MuSC is involved in regulating axonal fasciculation of mouse primary"Eur.J.Neurosci.. 18. 2244-2252 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka, D.: "Multimodal tangential migration of neocortical GABAergic neurons independent of GPI-anchored proteins"Development. 130. 5803-5813 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hattori, S.: "Quantitative relationship between Kv4.2 mRNA and A-type K^+ current in rat striatal cholinergic interneurons during development"J.Neurophysiol.. 90. 175-183 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 村上富士夫: "脳神経科学(伊藤正男 監修、金澤一郎 他 編)"三輪書店. 841(189-195) (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hatanaka, Y.: "In vitro analysis of the origin, migratory behavior and maturation of cortical pyramidal cells"J.Comp.Neurol.. 45. 1-14 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nagano, T.: "Filamin A-interacting protein FILIP regulates cortical cell migration out of the ventricular zone"Nature Cell Biology. 4. 495-501 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Taniguchi, H.: "Crossing the ventral midline causes neurons to change their response to floor plate and alar plate attractive cues during transmedian migration"Dev.Biol.. 249. 321-332 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takemoto, M.: "Ephrin-Be-EphA4 interactions regulate the growth of specific thalamocortical axon populations in vivo"Eur.J.Neurosci.. 16. 1168-1172 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 村上富士夫: "軸索の正中交叉のメカニズム-底板の誘引、反発と成長円錐反応性の制御"実験医学増刊 脳・神経研究のフロンティア. 20. 750-756 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 谷口弘樹: "脳における神経細胞移動の動態とそのメカニズム -器官培養系を用いた移動解析-"蛋白質核酸酵素. 47. 2002-2009 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 村上富士夫: "シリーズ バイオサイエンスの新世紀vol.11 脳の発生・分化・可塑性(御子柴克彦、清水孝雄 編)、133-144"脳の基本回路の形成機構. (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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