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Roles of Integrins of Endothelial Cells in Response to Mechanical Stimuli

Research Project

Project/Area Number 14208100
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionTohoku University

Principal Investigator

SATO Masaaki  Tohoku University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (30111371)

Co-Investigator(Kenkyū-buntansha) HANE Kazuhiro  Tohoku University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (50164893)
MATSUMOTO Takao  Nagoya Institute of Technology, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (30209639)
OHASHI Toshiro  Tohoku University, Graduate School of Engineering, Associate Professor, 大学院・工学研究科, 助教授 (30270812)
SAKAMOTO Naoya  Tohoku University, Graduate School of Engineering, Research Associate, 大学院・工学研究科, 助手 (20361115)
DEGUCHI Shinji  Okayama University, Faculty of Engineering, Research Associate, 工学部, 助手 (30379713)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥52,910,000 (Direct Cost: ¥40,700,000、Indirect Cost: ¥12,210,000)
Fiscal Year 2004: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2003: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2002: ¥21,710,000 (Direct Cost: ¥16,700,000、Indirect Cost: ¥5,010,000)
KeywordsBiomechanics / Integrin / Actin filament / Response mechanism to mechanical stimuli / Shear stress / Hydrostatic pressure / Tensile loading / Endothelial cell / 細胞骨格 / 伸張負荷 / GFP / FAT / 局所ひずみ
Research Abstract

In order to elucidate the response mechanisms of endothelial cells to mechanical stimuli, dynamic changes in actin filaments and integrins were precisely observed using confocal laser scanning microscopy. Shear stress, membrane tension of substrate and hydrostatic pressure as mechanical stimuli were applied to cultured endothelial cells and the following conclusions were obtained.
1.GFP-actin and RFP-FAT (focal adhesion targetting) vectors were transfected into cultured endothelial cells that were used to impose to shear stress. Actin filament was suggested to sense the tension and occur the dynamic changes as the first step. Secondly the lamellipodia were formed. During this process, it was suggested that the movement and allocation of integrins are possible to be controlled through microtubules.
2.Endothelial cells were cultured on silicone membrane in which a cover glass was immersed to form heterogeneous strain distribution. A cell was exposed to non-uniform strain and showed that distribution of actin filaments and the location of nucleus was dependent on the strain distribution. It was suggested that the cell is able to sense mechanical stress and respond so as to keep the mechanical balance in the cell.
3.We have reported the cultured endothelial cells respond to the hydrostatic pressure and show multilayering, cell elongation with no predominant orientation and characteristic distribution of actin filaments. To elucidate the mechanisms, we have focused on expression and distribution of VE-cadherin as an important protein to adhere the cells. It was suggested that the hydrostatic pressure has important effect on the expression of VE-cadherin.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (93 results)

All 2005 2004 2003 2002 Other

All Journal Article (64 results) Book (2 results) Patent(Industrial Property Rights) (1 results) Publications (26 results)

  • [Journal Article] Pipette aspiration method for the measurement of nonlinear and anisotropic mechanical properties of blood vessel walls under biaxial stretch2005

    • Author(s)
      T.Ohashi
    • Journal Title

      Journal of Biomechanics (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of actin filament distribution on tensile properties of smooth muscle cells obtained from rat thoracic aortas2005

    • Author(s)
      K.Nagayama
    • Journal Title

      Journal of Biomechanics (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Remodeling of vascular endothelial cells exposed to fluid shear stress : experimental and numerical approach2005

    • Author(s)
      T.Ohashi
    • Journal Title

      Fluid Dynamics Research (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pipette aspiration method for the measurement of nonlinear and anisotropic mechanical properties of blood vessel walls under biaxial stretch.2005

    • Author(s)
      T.Ohashi
    • Journal Title

      J Biomech. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of actin filament distribution on tensile properties of smooth muscle cells obtained from rat thoracic aortas.2005

    • Author(s)
      K.Nagayama
    • Journal Title

      J.Biomech. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Remodeling of vascular endothelial cells exposed to shear stress : experimental and numerical approach.2005

    • Author(s)
      T.Ohashi
    • Journal Title

      Fluid Dynamics Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 構造最適化による流れ負荷内皮細胞の有限要素法解析2005

    • Author(s)
      大橋俊朗
    • Journal Title

      日本機械学会論文集A編 (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Remodeling of Vascular Endothelial Cells Exposed to Fluid Shear Stress : Experimental and Numerical Approach2005

    • Author(s)
      T.Ohashi
    • Journal Title

      Fluid Dynamics Research (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Residual stress and strain in the lamellar unit of the porcine aorta : Experiment and analysis2004

    • Author(s)
      T.Matsumoto
    • Journal Title

      Journal of Biomechanics 37

      Pages: 807-815

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Do exercise-induced changes of distensibility and elastic components in rat aorta last for long after cessation of training?2004

    • Author(s)
      T.Nosaka
    • Journal Title

      International Journal of Sport and Health Science 2

      Pages: 76-83

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 細胞の力学特性計測のためのレーザ顕微鏡組込み型原子間力顕微鏡(AFM)システムの開発2004

    • Author(s)
      長山和亮
    • Journal Title

      日本機械学会論文集,C編 70(691)

      Pages: 736-742

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mechanical properties and function of the spinal pia mater2004

    • Author(s)
      H.Ozawa
    • Journal Title

      Journal of Neurosurgery (Spine 1) 1

      Pages: 122-127

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microscopic residual stress caused by the mechanical heterogeneity in the lamellar unit of the porcine thoracic aortic wall2004

    • Author(s)
      T.Matsumoto
    • Journal Title

      JSME Int.J.Ser.A 47(3)

      Pages: 341-348

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Quantitative estimation of brain stiffness measured using a tactile biosensor in animal models2004

    • Author(s)
      Y.Yamamoto
    • Journal Title

      Neurological Research 26(6)

      Pages: 622-627

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of shear stress on permeability of vascular endothelial monolayer cocultured with smooth muscle cells2004

    • Author(s)
      N.Sakamoto
    • Journal Title

      JSME Int.J. Ser.C 47(4)

      Pages: 992-999

    • NAID

      110004820828

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 溝造最適化による流れ負荷内皮細胞の有限要素法解析2004

    • Author(s)
      大橋俊朗
    • Journal Title

      日本機械学会論文集A編 70

      Pages: 1262-1268

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Residual stress and strain in the lamellar unit of the porcine aorta : Experiment and analysis.2004

    • Author(s)
      T.Matsumoto
    • Journal Title

      J.Biomech. 37(6)

      Pages: 807-815

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of newly designed atomic force microscope system for measuring of mechanical properties of cells. (in Japanese)2004

    • Author(s)
      K.Nagayama
    • Journal Title

      Trans.Jap.Soc.Mech.Eng.Ser.C 70(691)

      Pages: 736-742

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mechanical properties and function of the spinal pia mater.2004

    • Author(s)
      H.Ozawa
    • Journal Title

      Journal of Neurosurgery : spine 1

      Pages: 122-127

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microscopic residual stress caused by the mechanical heterogeneity in the lamellar unit of the porcine thoracic aortic wall.2004

    • Author(s)
      T.Matsumoto
    • Journal Title

      JSME Int.J.Ser.A 47(3)

      Pages: 341-348

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Quantitative estimation of brain stiffness measured using a tactile biosensor in animal models.2004

    • Author(s)
      Y.Yamamoto
    • Journal Title

      Neurological Research 26(6)

      Pages: 622-627

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of shear stress on permeability of vascular endothelial monolayer cocultured with smooth muscle cells.2004

    • Author(s)
      N.Sakamoto, T.Ohashi, M.Sato
    • Journal Title

      JSME Int.J.Ser.C 47(4)

      Pages: 992-999

    • NAID

      110004820828

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Finite element analysis for sheared vascular endothelial cells using structural optimization method. (in Japanese)2004

    • Author(s)
      T.Ohashi
    • Journal Title

      Trans.Jap.Soc.Mech.Eng.Ser.A 70

      Pages: 1262-1268

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of shear stress on permeability of vascular endothelial monolayer cocultured with smooth muscle cells2004

    • Author(s)
      N.Sakamoto
    • Journal Title

      JSME International Journal Series C Vol.47, No.4

      Pages: 992-999

    • NAID

      110004820828

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 静水圧に対する培養内皮細胞の形態的変化とその評価方法2004

    • Author(s)
      坂元尚哉
    • Journal Title

      第43回日本エム・イー学会大会プログラム・論文集 Vol.42 Suppl.1

      Pages: 589-589

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Role of nitric oxide in migration of smooth muscle cells in cocultured model2004

    • Author(s)
      N.Sakamoto
    • Journal Title

      The proceedings of The First Asian Pacific Conference on Biomechanics No.04-293

      Pages: 83-83

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 動脈硬化症発生メカニズムにおける内皮細胞と平滑筋細胞の相互作用2004

    • Author(s)
      坂元尚哉
    • Journal Title

      第27回日本バイオレオロジー学会年会プログラム・抄録集 18巻

      Pages: 40-40

    • NAID

      10012364336

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mechanical properties of a single stress fiber isolated from cultured smooth muscle cells2004

    • Author(s)
      M.Sato
    • Journal Title

      Proceeding of the 2004 BMES Annual Fall Meeting (CD)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 共存培養モデルを用いた平滑筋細胞遊走性に与える一酸化窒素の影響2004

    • Author(s)
      坂元尚哉
    • Journal Title

      日本機械学会第16回バイオエンジニアリング講演会講演論文集 No.03-38

      Pages: 31-32

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 培養動脈組織片の初期ひずみ状態が内皮細胞の形態および細胞骨格構造に及ぼす影響2003

    • Author(s)
      佐藤正明
    • Journal Title

      日本機械学会論文集(A編) 69(677)

      Pages: 30-36

    • NAID

      110002370268

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 平滑筋収縮に伴う家兎動脈壁内ひずみ分布の変化-弾性動脈と筋性動脈の比較-2003

    • Author(s)
      松本健郎
    • Journal Title

      日本機械学会論文集(A編) 69(677)

      Pages: 37-42

    • NAID

      110002370269

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 軸方向観察による血管壁の局所ひずみ分布計測2003

    • Author(s)
      杉田修啓
    • Journal Title

      日本機械学会論文集(A編) 69(677)

      Pages: 43-48

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 流れ刺激を受ける内皮細胞内骨格構造の実時間挙動観察2003

    • Author(s)
      大橋俊朗
    • Journal Title

      日本バイオレオロジー学会誌 17(1)

      Pages: 24-28

    • NAID

      130001932107

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 力学的刺激に対する血管内皮細胞の応答現象2003

    • Author(s)
      大橋俊朗
    • Journal Title

      生物物理 43

      Pages: 136-141

    • NAID

      110001157621

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oxygen tension modulates Ca^<2+> response to flow stimulus in endothelial cells exposed to hydrostatic pressure2003

    • Author(s)
      T.Ohashi
    • Journal Title

      Technology and Health Care 11

      Pages: 263-274

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Rupture properties of blood vessel walls measured by pressure-imposed test2003

    • Author(s)
      T.Ohashi
    • Journal Title

      JSME Int.J., Ser.C 46(4)

      Pages: 1290-1296

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Elongation and random orientation of bovine endothelial cells in response to hydrostatic pressure : comparison with response to shear stress2003

    • Author(s)
      Y.Sugaya
    • Journal Title

      JSME Int.J., Ser.C 46(4)

      Pages: 1248-1255

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Influence of regular exercise on age-related changes in arterial elasticity : mechanical insights from wall compositions in rat aorta2003

    • Author(s)
      T.Nosaka
    • Journal Title

      Can.J.Appl.Physiol. 28(2)

      Pages: 204-212

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of initial stretch conditions of cultured aortic wall segment on shape and cytoskeletal structure of endothelial cells. (in Japanese)2003

    • Author(s)
      M.Sato
    • Journal Title

      Trans.Jap.Soc.Mech.Eng.Ser.A 69(677)

      Pages: 30-36

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in strain distribution in rabbit arteries due to smooth muscle contraction (Comparison between elastic and muscular arteries). (in Japanese)2003

    • Author(s)
      T.Matsumoto
    • Journal Title

      Trans.Jap.Soc.Mech.Eng.Ser.A 69(677)

      Pages: 37-42

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Local strain measurement of arterial wall based on longitudinal observation. (in Japanese)2003

    • Author(s)
      S.Sugita
    • Journal Title

      Trans.Jap.Soc.Mech.Eng.Ser.A 69(677)

      Pages: 43-48

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Real-time observations of actin filament dynamics in cultured endothelial cells exposed to fluid flow. (in Japanese)2003

    • Author(s)
      T.Ohashi
    • Journal Title

      J.Jpn.Soc.Biorheol. 17(1)

      Pages: 24-28

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Remodeling of vascular endothelial cells exposed to mechanical stimuli. (in Japanese)2003

    • Author(s)
      T.Ohashi
    • Journal Title

      Seibutsu Butsuri 43

      Pages: 136-141

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oxygen tension modulates Ca^<2+> response to flow stimulus in endothelial cells exposed to hydrostatic pressure.2003

    • Author(s)
      T.Ohashi
    • Journal Title

      Technol.Health Care 11

      Pages: 263-274

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Rupture properties of blood vessel walls measured by pressure-imposed test.2003

    • Author(s)
      T.Ohashi
    • Journal Title

      JSME Int.J., Ser C 46(4)

      Pages: 1290-1296

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Elongation and random orientation of bovine endothelial cells in response to hydrostatic pressure : comparison with response to shear stress.2003

    • Author(s)
      Y.Sugaya
    • Journal Title

      JSME Int.J., Ser C 46(4)

      Pages: 1248-1255

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Influence of regular exercise on age-related changes in arterial elasticity : mechanical insights from wall compositions in rat aorta.2003

    • Author(s)
      T.Nosaka
    • Journal Title

      Can.J.Appl.Physiol. 28(2)

      Pages: 204-212

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ビタミンEの白血球粘着抑制効果および血小板凝集抑制作用2002

    • Author(s)
      佐藤正明
    • Journal Title

      医学と薬学 47(3)

      Pages: 457-461

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] MRI画像に基づいた簡便な胸部大動脈拘束条件推定方法と胸部大動脈瘤への適用2002

    • Author(s)
      加藤陽子
    • Journal Title

      脈管学 42(4)

      Pages: 247-253

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Deformation properties of articular chondrocytes : A critique of three separate techniques2002

    • Author(s)
      D.L.Bader
    • Journal Title

      Biorheology 39

      Pages: 69-78

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Targeted disruption of dermatopontin causes abnormal collagen fibrillogenesis2002

    • Author(s)
      U.Takeda
    • Journal Title

      Journal of Investigative Dermatology 119

      Pages: 678-683

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Experimental and numerical analyses of local mechanical properties measured by atomic force microscopy for sheared endothelial cells2002

    • Author(s)
      T.Ohashi
    • Journal Title

      Bio-medical Materials and Engineering 12

      Pages: 319-327

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Measurements of endothelial cell-to-cell and cell-to-substrate gaps and micro-mechanical properties of endothelial cells during monocytes adhesion2002

    • Author(s)
      N.Kataoka
    • Journal Title

      Proceedings of National Academy of Science 99(24)

      Pages: 15638-15643

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Local elastic modulus of atherosclerotic lesions of rabbit thoracic aortas measured by pipette aspiration method2002

    • Author(s)
      T.Matsumoto
    • Journal Title

      Physiological Measurement 23

      Pages: 635-648

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysis of stress and strain distribution in the artery wall consisted of layers with different elastic modulus and opening angle2002

    • Author(s)
      T.Matsumoto
    • Journal Title

      JSME Int J, Ser C 45(4)

      Pages: 906-912

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of magnetic field on nitric oxide synthesis of cultured endothelial cells2002

    • Author(s)
      N.Sakamoto
    • Journal Title

      International Journal Applied Electromagnetics and Mechanics 14

      Pages: 317-322

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Vitamin E inhibits leukocyte adhesion and platelet aggregation.(in Japanese)2002

    • Author(s)
      M.Sato
    • Journal Title

      Jpn.J.Med.Pharm.Sci. 47(3)

      Pages: 457-461

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A simple method to estimate the constraints of thoracic aorta model based on MRI images and its application to the thoracic aortic aneurysm model. (in Japanese)2002

    • Author(s)
      Y.Kato
    • Journal Title

      Journal of Japanese College of Angiology 42(4)

      Pages: 247-253

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Deformation properties of articular chondrocytes : A critique of three separate techniques.2002

    • Author(s)
      D.L.Bader
    • Journal Title

      Biorheology 39

      Pages: 69-78

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Targeted Disruption of dermatopontin causes abnormal collagen fibrillogenesis2002

    • Author(s)
      U.Takeda
    • Journal Title

      The Journal of Investigative Dermatology 119

      Pages: 678-683

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Experimental and numerical analyzes of local mechanical properties measured by atomic force microscopy for sheared endothelial cells.2002

    • Author(s)
      T.Ohashi
    • Journal Title

      Bio-medical Materials and Engineering 12

      Pages: 319-327

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Measurements of endothelial cell-to-cell and cell-to-substrate gaps and micro-mechanical properties of endothelial cells during monocytes adhesion.2002

    • Author(s)
      N.Kataoka
    • Journal Title

      Proceedings of National Academy of Science 99(24)

      Pages: 15638-15643

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Local elastic modulus of atherosclerotic lesions of rabbit thoracic aortas measured by pipette aspiration method.2002

    • Author(s)
      T.Matsumoto
    • Journal Title

      Physiological Measurement 23

      Pages: 635-648

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of magnetic field on nitric oxide synthesis of cultured endothelial cells.2002

    • Author(s)
      N.Sakamoto
    • Journal Title

      International Journal Applied Electromagnetics and Mechanics 14

      Pages: 317-322

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 構造工学ハンドブック(13章1・2 細胞構造)2004

    • Author(s)
      佐藤正明
    • Total Pages
      1070
    • Publisher
      丸善
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Book] Chap.13, 1.2 Cell Structure Handbook of Structural Engineering2004

    • Author(s)
      M.Sato
    • Total Pages
      1070
    • Publisher
      Maruzen Co.Ltd.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 解離性大動脈瘤用治療プラグ2002

    • Inventor(s)
      江刺 正喜
    • Industrial Property Rights Holder
      (株)東北テクノアーチ
    • Industrial Property Number
      2002-213767
    • Filing Date
      2002-07-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 佐藤正明: "培養動脈組織片の初期ひずみ状態が内皮細胞の形態および細胞骨格構造に及ぼす影響"日本機械学会論文集(A編). 69・677. 30-36 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 松本健郎: "平滑筋収縮に伴う家兎動脈壁内ひずみ分布の変化-弾性動脈と筋性動脈の比較-"日本機械学会論文集(A編). 69・677. 37-42 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 杉田修啓: "軸方向観察による血管壁の局所ひずみ分布計測"日本機械学会論文集(A編). 69・677. 43-48 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 大橋俊朗: "流れ刺激を受ける内皮細胞内骨格構造の実時間挙動観察"日本バイオレオロジー学会誌. 17・1. 24-28 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Ohashi: "Oxygen tension modulates Ca^<2+> response to flow stimulus in endothelial cells exposed to hydrostatic pressure"Technology and Health Care. 11. 263-274 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 大橋俊朗: "力学的刺激に対する血管内皮細胞の応答現象"生物物理. 43・3. 136-141 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Ohashi: "Rupture properties of blood vessel walls measured by pressure-imposed test"JSME Int.J.,Ser.C. 46・4. 1290-1296 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Sugaya: "Elongation and random orientation of bovine endothelial cells in response to hydrostatic pressure : comparison with response to shear stress"JSME Int.J.,Ser.C. 46・4. 1248-1255 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Ohashi: "Pipette aspiration method for the measurement of nonlinear and anisotropic mechanical properties of blood vessel walls under biaxial stretch"Journal of Biomechanics. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Matsumoto: "Residual stress and strain in the lamellar unit of the porcine aorta : Experiment and analysis"Journal of Biomechanics. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 長山和亮: "細胞の力学特性計測のためのレーザ顕微鏡組込み型原子間力顕微鏡(AFM)システムの開発"日本機会学会論文集,C編. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤正明: "構造工学ハンドブック(13章1・2 細胞構造)"丸善. 1070 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤正明: "ビタミンEの白血球粘着抑制効果および血小板擬集抑制作用"医学と薬学. 47巻3号. 457-461 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 加藤陽子: "MRI画像に基づいた簡便な胸部大動脈拘束条件推定方法と胸部大動脈瘤への適用"脈管学. 42巻4号. 247-253 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] D.L.Bader: "Deformation properties of articular chondrocytes : A critique of three separate techniques"Biorheology. 39巻. 69-78 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] U.Takeda: "Targeted disruption of dermatopontin causes abnormal collagen fibrillogenesis"Journal of Investigative Dermatology. 119巻. 678-683 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Ohashi: "Experimental and numerical analyses of Local mechanical properties measured by atomic force microscopy for sheared endothelial cells"Bio-medical Materials and Engineering. 12巻. 319-327 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] N.Kataoka: "Measurements of endothelial cell-to-cell and cell-to-substrate gaps and micromechanical properties of endothelial cells during monocytes adhesion"Proceedings of National Academy of Science. 99巻24号. 15638-15643 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 佐藤正明: "培養動脈組織片の初期ひずみ状態が内皮細胞の形態および細胞骨格構造に及ぼす影響"日本機械学会論文集(A編). 69巻677号. 30-36 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 松本健郎: "平滑筋収縮に伴う家兎動脈壁内ひずみ分布の変化-弾性動脈と筋性動脈の比較-"日本機械学会論文集(A編). 69巻677号. 37-42 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 杉田修啓: "軸方向観察による血管壁の局所ひずみ分布計測"日本機械学会論文集(A編). 69巻677号. 43-48 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Matsumoto: "Analysis of stress and strain distribution in the artery wall consisted of layers with different elastic modulus and opening angle"JSME Int J, Ser C. 45巻4号. 906-912 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Matsumoto: "Local elastic modulus of atherosclerotic lesions of rabbit thoracic aortas measured by pipette aspiration method"Physiological Measurement. 23巻. 635-648 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] N.Sakamoto: "Effect of magnetic field on nitric oxide synthesis of cultured endothelial cells"International Journal Applied Electromagnetics and Mechanics. 14巻. 317-322 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大橋俊朗: "流れ刺激を受ける内皮細胞内骨格構造の実時間挙動観察"日本バイオレオロジー学会誌. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大橋俊朗: "力学的刺激に対する血管内皮細胞の応答現象"生物物理. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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