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Expansion and differentiation of marrow mesenchymal stem cells in porous ceramics

Research Project

Project/Area Number 14208107
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionNATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Principal Investigator

OHGUSHI Hajime  NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY (AIST), TISSUE ENGINEERING RESEARCH GROUP RESEARCH INSTITUTE FOR CELL ENGINEERING, GROUP LEADER, セルエンジニアリング研究部門, 研究グループ長 (80213669)

Co-Investigator(Kenkyū-buntansha) ITO Atsuo  NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY (AIST), 主任研究員 (30356480)
KODAMA Makoto  KYUSYU INSTITUTE OF TECHNOLOGY, GRADUATE SCHOOL OF LIFE SCIENCE AND SYSTEMS ENGINEERING, 大学院・生命体工学研究科, 助教授 (70344447)
KANEMURA Yonehiro  NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY (AIST), 研究員 (80344175)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥54,210,000 (Direct Cost: ¥41,700,000、Indirect Cost: ¥12,510,000)
Fiscal Year 2004: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2003: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2002: ¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
KeywordsMesenchymal stem cells / Bone marrow / Bone regeneration / Tissue engineering / Culture / Hepatocyte / Neuron
Research Abstract

Stem cells have a remarkable potential for developing into many different cell types in the body. When a stem cell divides, each new cell has the ability to either remain a stem cell (self-renewal) or become another type of cell with a more specialized function (specific cell lineage commitment). Stem cells derived from postnatal animals (especially humans) are called adult stem cells and are generally thought to be multipotent stem cells.
Among adult stem cells, mesenchymal stem cells (MSCs) are also multipotent cells that can be isolated from adult bone marrow and can be induced in vitro and in vivo to differentiate into a variety of mesenchymal tissues, including bone and cartilage. Here, we report that we have succeeded to proliferate the number of MSCs from adult human as well as rat and also induced theses MSCs into osteo/chondrogenic lineage. Importantly, the osteogenic cells in the pore regions of various porous ceramics can be used to repair osseous defects and we have used the cells for the clinical applications.
Interestingly, the MSCs can also differentiate into hepatocyte and neuron. When MSCs were cultured in the presence of hepatocyte growth factor, the cells showed albumin and c-Kit expression. When the MSCs were cultured in the presence of mercapto-ethanol, the cells' morphorogy resembled neuron and showed Nestin expression. Therefore, MSCs derived from bone marrow can be used to regenerate hepatic and neural function. These results indicate the usefulness of MSCs for tissue engineering and, thus, the cells hold promise for utilization in regenerative medicine.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (90 results)

All 2005 2004 2003 2002 Other

All Journal Article (67 results) Book (13 results) Publications (10 results)

  • [Journal Article] Morphology of Porous Hydroxyapatite Scaffold for Hard Tissue Formation.2005

    • Author(s)
      Yoshikawa M, Tsuji N, Toda T, Machida H, Ohgushi H.
    • Journal Title

      Key Eng Mater 17

      Pages: 321-324

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Culturing Human Mesenchymal Stem Cells on Bioceramics for Hard Tissue Regeneration (Validation of In Vitro Bone Matrix Formed on Ceramics for Clinical Application).2005

    • Author(s)
      Ohgushi H, Kitamura S, Kotobuki N, Hirose M, Machida H, Oshima A, et al.
    • Journal Title

      Key Eng Mater 17

      Pages: 603-606

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In Vitro Osteogenic Activity of Rat Mesenchymal Cells Cultured on Transparent β-Tricalcium Phosphate Ceramics.2005

    • Author(s)
      Kotobuki N, Ioku K, Kawagoe D, Nomura D, Fujimori H, Goto S et al.
    • Journal Title

      Key Eng Mater 17

      Pages: 663-666

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Comparison of Hard Tissue Formation in Two Porous Hydroxyapatite Scaffolds Treated with Hyaluronic Acid Sodium Salt.2005

    • Author(s)
      Tsuji N, Yoshikawa M, Toda T, Machida H, Ohgushi H.
    • Journal Title

      Key Eng Mater 17

      Pages: 961-964

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Comparison of Recombinant Growth/Differentiation Factor-5 (rGDF-5) and Bone Morphogenetic Protein-2 (rBMP-2) in the In Vivo Bone Formation in Porous Hydroxyapatite Ceramic.2005

    • Author(s)
      Shimaoka H, Dohi Y, Narikawa K, Ohgushi H, Ikeuchi M, Okamoto M, et al.
    • Journal Title

      Key Eng Mater 17

      Pages: 945-948

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Potentiation of the activity of bone morphogenetic protein-2 in bone regeneration by a PLA-PEG/hydroxyapatite composite.2005

    • Author(s)
      Kaito T, Myoui A, Takaoka K, Saito N, Nishikawa M, Tamai N, Ohgushi H, Yoshikawa H.
    • Journal Title

      Biomaterials 26(1)

      Pages: 73-79

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Observation of osteogenic differentiation cascade of living mesenchymal stem cells on transparent hydroxyapatite ceramics.2005

    • Author(s)
      Kotobuki N, Ioku K, Kawagoe D, Fujimori H, Goto S, Ohgushi H.
    • Journal Title

      Biomaterials 26(7)

      Pages: 779-785

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture.2005

    • Author(s)
      Dohi Y, Shimaoka H, Ikeuchi M, Ohgushi H, Yonemasu K, Minami T.
    • Journal Title

      Biol Trace Elem Res 104(1)

      Pages: 57-70

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly-lactic-glycolic acid (PLGA) scaffold.2005

    • Author(s)
      Uematsu K, Hattori K, Ishimoto Y, Yamauchi J, Habata T, Takakura Y, et al.
    • Journal Title

      Biomaterials 26(20)

      Pages: 4273-4279

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tissue engineered ceramic artificial joint-ex vivo osteogenic differentiation of patient mesenchymal cells on total ankle joints for treatment of osteoarthritis.2005

    • Author(s)
      Ohgushi H, Kotobuki N, Funaoka H, Machida H, Hirose M, Tanaka Y, et al.
    • Journal Title

      Biomaterials 26(22)

      Pages: 4654-4661

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Morphology of Porous Hydroxyapatite Scaffold for Hard Tissue Formation.2005

    • Author(s)
      Yoshikawa M, Tsuji N, Toda T, Machida H, Ohgushi H.
    • Journal Title

      Key Eng Mater 284-286

      Pages: 321-324

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Culturing Human Mesenchymal Stem Cells on Bioceramics for Hard Tissue Regeneration (Validation of In Vitro Bone Matrix Formed on Ceramics for Clinical Application).2005

    • Author(s)
      Ohgushi H, Kitamura S, Kotobuki N, Hirose M, Machida H, Oshima A, et al.
    • Journal Title

      Key Eng Mater 284-286

      Pages: 603-606

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic Activity of Rat Mesenchymal Cells Cultured on Transparent 6-Tricalcium Phosphate Ceramics.2005

    • Author(s)
      Kotobuki N, Ioku K, Kawagoe D, Nomura D, Fujimori H, Goto S, et al.
    • Journal Title

      Key Eng Mater 284-246

      Pages: 663-666

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Comparison of Hard Tissue Formation in Two Porous Hydroxyapatite Scaffolds Treated with Hyaluronic Acid Sodium Salt.2005

    • Author(s)
      Tsuji N, Yoshikawa M, Toda T, Machida H, Ohgushi H.
    • Journal Title

      Key Eng Mater 284-286

      Pages: 961-964

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Comparison of Recombinant Growth/Differentiation Factor-5 (r-GDF-5) and Bone Morphogenetic Protein-2 (rBMP-2) in the In Vivo Bone Formation in Porous Hydroxyapatite Ceramic.2005

    • Author(s)
      Shimaoka H, Dohi Y, Narikawa K, Ohgushi H, Ikeuchi M, Okamoto M, et al.
    • Journal Title

      Key Eng Mater 284-286

      Pages: 945-948

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly-lactkrglycolic acid(PLGA) scaffold.2005

    • Author(s)
      Uematsu K, Hattori K, Ishimoto Y, Yamauchi J, Habata T, Takakura Y, et al.
    • Journal Title

      Biomaterials 26(20)

      Pages: 4273-4279

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Observation of osteogenic differentiation cascade of living mesenchymal stem cells on transparent hydroxyapatite ceramics2005

    • Author(s)
      Kotobuki N, et al.
    • Journal Title

      Biomaterials 26(1)

      Pages: 73-79

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Tissue engineered ceramic artificial joint-ex vivo osteogenic differentiation of patient mesenchymal cells on total ankle joint for treatment of osteoarthritis2005

    • Author(s)
      Ohgushi H, et al.
    • Journal Title

      Biomaterials 26(22)

      Pages: 4654-4661

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Three-dimensionai visualization analysis of in vitro cultured bone fabricated by rat marrow mesenchymal stem cells.2005

    • Author(s)
      Kihara T, et al.
    • Journal Title

      Biochem Biophys Res Commun 316(3)

      Pages: 943-948

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Clinical application of marrow mesenchymal stem cells for hard tissue repair.2005

    • Author(s)
      Ohgushi H, et al.
    • Journal Title

      Yonsei Med J 2004 45 Suppl

      Pages: 61-67

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cultured autologous human cells for hard tissue regeneration : preparation and characterization of mesenchymal stem cells from bone marrow.2004

    • Author(s)
      Kotobuki, N., Hirose M., Takakura Y., Ohgushi, H
    • Journal Title

      Artificial Organs 28(1)

      Pages: 33-39

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic differentiation of human bone marrow-derived mesenchymal cells cultured on alumina ceramics.2004

    • Author(s)
      Kitamura, S., Ohgushi, H., Hirose, M., Funaoka, H, Takakura, Y., Ito, H.
    • Journal Title

      Artificial Organs 28(1)

      Pages: 72-81

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Recombinant growth/differentiation factor-5 (GDF-5) stimulates osteogenic differentiation of marrow mesenchymal cells in porous hydroxyapatite ceramic.2004

    • Author(s)
      Shimaoka, H, Dohi, Y, Ohgushi, H., Ikeuchi, M., Okamoto, M., Kudo, A., Kirita, T., Yonemasu, K.
    • Journal Title

      Journal of Biomedical Materials Research 68A(1)

      Pages: 168-176

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic Activity of Human Marrow Cells on Alumina Ceramics.2004

    • Author(s)
      Tanaka Y, Ohgushi H, Kitamura S, Taniguchi A, Hayashi K, Isomoto S, Tohma Y, Takakura Y.
    • Journal Title

      Key Eng Mater 16

      Pages: 254-256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic potential of cryopreserved human bone marrow-derived mesenchymal cells after thawing in culture.2004

    • Author(s)
      Hirose M, Kotobuki N, Machida H, Kitamura S, Ohgushi H, Tateishi T.
    • Journal Title

      Mat Sci Eng C-Bio S 2004 24(3)

      Pages: 335-359

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In Vitro Osteogenic Activity of Rat Bone Marrow Derived Mesenchymal Stem Cells Cultured on Transparent Hydroxyapatite Ceramics.2004

    • Author(s)
      Noriko Kotobuki DK, Hirotaka Fujimori, Seishi Goto, Koji Ioku, Hajime Ohgushi.
    • Journal Title

      Key Eng Mater 16

      Pages: 1055-1058

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Three-dimensional visualization analysis of in vitro cultured bone fabricated by rat marrow mesenchymal stem cells.2004

    • Author(s)
      Kihara T, Oshima A, Hirose M, Ohgushi H.
    • Journal Title

      Biochem Biophys Res Commun 3

      Pages: 943-948

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic potential of cryopreserved human bone marrow-derived mesenchymal cells after thawing in culture.2004

    • Author(s)
      Hirose M, Kotobuki N, Machida H, Kitamura S, Ohgushi H, Tateishi T.
    • Journal Title

      Mat Sci Eng C-Bio S 24(3)

      Pages: 355-359

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bone regeneration by grafting of cultured human bone.2004

    • Author(s)
      Yoshikawa T, Ohgushi H, Ichijima K, Takakura Y.
    • Journal Title

      Tissue Eng 2004 10

      Pages: 688-698

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinical application of marrow mesenchymal stem cells for hard tissue repair.2004

    • Author(s)
      Ohgushi H, Kitamura S, Kotobuki N, Hirose M, Machida H, Muraki K, Takakura Y.
    • Journal Title

      Yonsei Med J 45

      Pages: 61-67

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Which cartilage is regenerated, hyaline cartilage or fibrocartilage? Non-invasive ultrasonic evaluation of tissue engineered cartilage.2004

    • Author(s)
      Hattori K, Takakura Y, Ohgushi H, Habata T, Uematsu K, Takenaka M, Ikeuchi K.
    • Journal Title

      Rheumatology (Oxford) 43(9)

      Pages: 1106-1108

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ankle arthroplasty using three generations of metal and ceramic prostheses.2004

    • Author(s)
      Takakura Y, Tanaka Y, Kumai T, Sugimoto K, Ohgushi H.
    • Journal Title

      Clin Orthop 2004 424

      Pages: 130-136

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Growth factor combination for chondrogenic induction from human mesenchymal stem cell.2004

    • Author(s)
      Indrawattana N, Chen G, Tadokoro M, Shann LH, Ohgushi H, Tateishi T, Tanaka J, Bunyaratvej A.
    • Journal Title

      Biochem Biophys Res Commun 320(3)

      Pages: 914-919

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of the cDNA encoding bullfrog, Rana catesbeiana, osteocalcin and two forms of the protein isolated from bone.2004

    • Author(s)
      Dohi Y, Tabata S, Yamaguchi M, Ohgushi H
    • Journal Title

      Yonemasu K. Biochimie 86(7)

      Pages: 471-480

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic potential of cultured bane/ceramic construct : comparison with marrow mesenchymal cell/ceramic composite.2004

    • Author(s)
      Iida J, Yoshikawa T, Akahane M, Ohgushi H, Dohi Y, Takakura Y, Nonomura A.
    • Journal Title

      Cell Transplant 13(4)

      Pages: 357-365

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bone tissue engineering using novel interconnected porous hydroxyapatite ceramics combined with marrow mesenchymal cells - Quantitative and three-dimensional image analysis -.2004

    • Author(s)
      Nishikawa M, Ohgushi H, Myoui A, Ikeuchi M, Tamai N, Yoshikawa H.
    • Journal Title

      Cell Transplant 13(4)

      Pages: 367-376

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enhancement of in vitro osteoblastic potential after selective sorting of osteoblasts with high alkaline phosphatase activity from human osteoblast-like cells.2004

    • Author(s)
      Kotobuki, N., Hirose, M., Funaoka, H., Ohgushi, H.
    • Journal Title

      Cell Transplant 13(4)

      Pages: 377-383

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chondrogenic differentiation of human mesenchymal stem cells cultured in a cobweb-like biodegradable scaffold.2004

    • Author(s)
      Chen G, Liu D, Tadokoro M, Hirochika R, Ohgushi H, Tanaka J, et al.
    • Journal Title

      Biochem Biophys Res Commun 322(1)

      Pages: 50-55

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cell adhesion of bone marrow cells, chondrocytes, ligament cells and synovial cells on a PLGA-collagen hybrid mesh.2004

    • Author(s)
      Chen G, Liu D, Maruyama N, Ohgushi H, Tanaka J, Tateishi T.
    • Journal Title

      Mat Sci Eng C-Bio S 24(6-8)

      Pages: 701-926

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cultured autologous human cells for hard tissue regeneration : preparation and characterization of mesenchymal stem cells from bone marrow.2004

    • Author(s)
      Kotobuki N, Hirose M, Takakura Y, Ohgushi H.
    • Journal Title

      Artif Organs 28(1)

      Pages: 33-39

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic differentiation of human bone marrow-derived mesenchymal cells cultured on alumina ceramics.2004

    • Author(s)
      Kitamura S, Ohgushi H, Hirose M, Funaoka H, Takakura Y, Ito H.
    • Journal Title

      Artif Organs 28(1)

      Pages: 72-82

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Recombinant growth/differentiation factor-5(GDF-5) stimulates osteogenic differentiation of marrow mesenchymal stem cells in porous hydroxyapatite ceramic.2004

    • Author(s)
      Shimaoka H, Dohi Y, Ohgushi H, Ikeuchi M, Okamoto M, Kudo A, Kirita T, Yonemasu K.
    • Journal Title

      J Biomed Mater Res 68A(1)

      Pages: 168-176

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic Activity of Human Marrow Cells on Alumina Ceramics.2004

    • Author(s)
      Tanaka Y, Ohgushi H, Kitamura S, Taniguchi A, Hayashi K, Isomoto S, Tohma Y, Takakura Y.
    • Journal Title

      Key Eng Mater 254-256

      Pages: 181-184

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In Vitro Osteogenic Potential of Cryopreserved/Thawed Human Bone Marrow-Derived Mesenchymal Stem Cells.2004

    • Author(s)
      Hirose M, Kotobuki N, Machida H, Kitamura S, Takakura Y, Ohgushi H.
    • Journal Title

      Key Eng Mater 254-256

      Pages: 1051-1054

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic Activity of Rat Bone Marrow Derived Mesenchymal Stem Cells Cultured on Transparent Hydroxyapatite Ceramics.2004

    • Author(s)
      Noriko Kotobuki DK, Hirotaka Fujimori, Seishi Goto, Koji Ioku, Hajime Ohgushi
    • Journal Title

      Key Eng Mater 254-256

      Pages: 1055-1058

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Three-dimensional visualization analysis of in vitro cultured bone fabricated by rat marrow mesenchymal stem cells.2004

    • Author(s)
      Kihara T, Oshima A, Hirose M, Ohgushi H.
    • Journal Title

      Biochem Biophys Res Commun 316(3)

      Pages: 943-948

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bone regeneration by grafting of cultured human bone.2004

    • Author(s)
      Yoshikawa T, Ohgushi H, Ichijima K, Takakura Y.
    • Journal Title

      Tissue Eng 10(5-6)

      Pages: 688-698

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinical application of marrow mesenchymal stem cells for hard tissue repair.2004

    • Author(s)
      Ohgushi H, Kitamura S, Kotobuki N, Hirose M, Machida H, Muraki K, Takakura Y.
    • Journal Title

      Yonsei Med J 45 Suppl

      Pages: 61-67

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Which cartilage is regenerated, hyaline cartilage or fibrocartilage? Non-invasive ultrasonic evaluation of tissue-engineered cartilage.2004

    • Author(s)
      Hattori K, Takakura Y, Ohgushi H, Habata T, Uematsu K, Takenaka M, Ikeuchi K.
    • Journal Title

      Rheumatology (Oxford) 43(9)

      Pages: 1106-1108

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ankle arthroplasty using three generations of metal and ceramic prostheses.2004

    • Author(s)
      Takakura Y, Tanaka Y, Kumai T, Sugimoto K, Ohgushi H.
    • Journal Title

      Clin Orthop (424)

      Pages: 130-136

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of the cDNA encoding bullfrog, Rana catesbeiana, osteocalcin and two forms of the protein isolated from bone.2004

    • Author(s)
      Dohi Y, Tabata S, Yamaguchi M, Ohgushi H, Yonemasu K.
    • Journal Title

      Biochimie 86(7)

      Pages: 471-480

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic potential of cultured bone/ceramic construct : comparison with marrow mesenchymal cell/ceramic composite.2004

    • Author(s)
      Iida J, Yoshikawa T, Akahane M, Ohgushi H, Dohi Y, Takakura Y, Nonomura A.
    • Journal Title

      Cell Transplant 13(4)

      Pages: 357-365

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bone tissue engineering using novel interconnected porous hydroxyapatite ceramics combined with marrow mesenchymal cells-Quantitative and three-dimensional image analysis-.2004

    • Author(s)
      Nishikawa M, Ohgushi H, Myoui A, Ikeuchi M, Tamai N, Yoshikawa H.
    • Journal Title

      Cell Transplant 13(4)

      Pages: 367-376

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enhancement of in vitro osteoblastic potential after selective sorting of osteoblasts with high alkaline phosphatase activity from human osteoblast-like cells.2004

    • Author(s)
      Kotobuki N, et al.
    • Journal Title

      Cell Transplant 13(4)

      Pages: 377-383

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Transplantation of Hepatocytes Cultured on Hydroxyapatite into Nagase Analbuminemia Rats.2003

    • Author(s)
      Shinji Higashiyama, Megumi Noda, Satoko Muraoka, Motohiro Hirose, Hajime Ohgushi, Masaya Kawase, Kiyohito Yagi
    • Journal Title

      Journal of Bioscience and Bioengineering 196

      Pages: 83-85

    • NAID

      110002665157

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic differentiation of cultured rat and human bone marrow cells on the surface of zinc-releasing calcium phosphate ceramics.2003

    • Author(s)
      Ikeuchi, M., Ito, A., Dohi, Y., Ohgushi, H., Shimaoka, H., Yonemasu, K., Tateishi, T.
    • Journal Title

      Journal of Biomedical Materials Research 67A(4)

      Pages: 1115-1122

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In-situ visualization and quantification of mineralization of cultured osteogenetic cells.2003

    • Author(s)
      Uchimura, E., Machida, H., Kotobuki, N., Kihara, T., Kitamura, S., Ikeuchi, M., Hirose, M., Miyake, J., Ohgushi, H.
    • Journal Title

      Calcified Tissue International 73(6)

      Pages: 575-583

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transplantation of Hepatocytes Cultured on Hydroxyapatite into Nagase Analbuminemia Rats2003

    • Author(s)
      Shinji Higashiyama, Megumi Noda, Satoko Muraoka, Motohiro Hirose, Hajime Ohgushi, Masaya Kawase, Kiyohito Yagi
    • Journal Title

      Journal of Bioscience and Bioengineering Vol.96 No.1

      Pages: 83-85

    • NAID

      110002665157

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic differentiation of cultured rat and human bone marrow cells on the surface of zinc-releasing calcium phosphate ceramics.2003

    • Author(s)
      Ikeuchi, M., Ito, A., Dohi, Y., Ohgushi, H., Shimaoka, H., Yonemasu, K., Tateishi, T.
    • Journal Title

      Journal of Biomedical Materials Research Part 67A(4)

      Pages: 1115-1122

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Recombinant Human Bone Morphogenetic Protein-2 Promotes Osteogenesis within Atelopeptide Type I Collagen Solution by Combination with Rat Cultured Marrow Cells.2002

    • Author(s)
      M.Ikeuchi, Y Dohi, K Horiuchi, H Ohgushi, T.Noshi, T.Yoshikawa, K.Yamamoto, M Sugimura
    • Journal Title

      Journal of Biomedical Materials Research 60

      Pages: 61-69

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enhancing and suppressing effects of dexamethasone on transgene expression in vitro.2002

    • Author(s)
      M.Akahane, S.Kuriyama, H.Ohgushi, T.Akahane, K.Kawamura, S.Watanabe, F.Funaoka, H.Yoshiji, K.Ikenaka, Y.Takakura
    • Journal Title

      International Journal of Molecular Medicine 10

      Pages: 107-112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hydroxyapatite ceramics as a cariier of gene-transduced bone marrow cells.2002

    • Author(s)
      M.Akahane, H.Ohgushi, S.Kuriyama, T.Akahane, Y.Takakura
    • Journal Title

      Journal of Orthopedic Science 17 No.6

      Pages: 1677-1682

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Zinc-releasing calcium phosphate for stimulateing bone formation.2002

    • Author(s)
      A.Ito, H.Kawamura, M.Otsuka, M.Ikeuchi, H.Ohgushi, K Ishikawa, K Onuma, N Kanzaki, N.Ichinose
    • Journal Title

      Materials Science and Engineering C 22

      Pages: 21-25

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Recombinant Human Bone Morphogenetic Protein-2 Promotes Osteogenesis within Atelopeptide Type I Collagen Solution by Combination with Rat Cultured Marrow Cells.2002

    • Author(s)
      M.Ikeuchi, Y Dohi, K Horiuchi, H Ohgushi, T.Noshi, T.Yoshikawa, K.Yamamoto, M Sugimura
    • Journal Title

      Journal of Biomedical Materials Research Vol.60

      Pages: 61-69

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enhancing and suppressing effects of dexamethasone on transgene expression in vitro.2002

    • Author(s)
      M.Akahane, S.Kuriyama, H.Ohgushi, T.Akahane, K.Kawamura, S.Watanabe, F.Funaoka, H.Yoshiji, K.Ikenaka, Y.Takakura
    • Journal Title

      International Journal of Molecular Medicine Vol.10

      Pages: 107-112

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hydroxyapatite ceramics as a cariier of gene-transduced bone marrow cells.2002

    • Author(s)
      M.Akahane, H.Ohgushi, S.Kuriyama, T.Akahane, Y.Takakura
    • Journal Title

      Journal of Orthopedic Science Vol.7 No.6

      Pages: 677-682

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Zinc-releasing calcium phosphate for stimulateing bone formation.2002

    • Author(s)
      A.Ito, H.Kawamura, M.Otsuka, M.Ikeuchi, H.Ohgushi.K Ishikawa, K Onuma, N Kanzaki, N.Ichinose
    • Journal Title

      Materials Science and Engineering C Vol.22 No.1

      Pages: 21-25

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Transactions of the Materials Research Society of Japan2004

    • Author(s)
      Hirose, M., Kotobuki, N., Machida, H., Kitamura, S., Takakura, Y., Ohgushi, H.
    • Publisher
      IUMRS-ICAM
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Bioceramics152003

    • Author(s)
      M.Hirose, N.Kotobuki, H.Machida, E.Uchimura, H.Ohgushi
    • Publisher
      TRANS TECH PUBLICATIONS
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Bioceramics152003

    • Author(s)
      N.Kotobuki, M.Hirose, H.Funaoka, H.Ohgushi
    • Publisher
      TRANS TECH PUBLICATIONS
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Bioceramics152003

    • Author(s)
      Ohgushi, M.Ikeuchi, T.Tateishi, Y.Tohma, T.Tanaka, T.Takakura
    • Publisher
      TRANS TECH PUBLICATIONS
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Bioceramics152003

    • Author(s)
      Y.Thoma, H.Ohgushi, Y.Dohi, H.SHimaoka, Y.Ikeda, Y.Mizuta, T.Takakura
    • Publisher
      TRANS TECH PUBLICATIONS
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Bioceramics152003

    • Author(s)
      Y.Tanaka, Y.Takakura, K.Kadono, A.Taniguchi, K.Hayashi, J.Iida, K.Sugimoto, Y.Thoma, H.Ohgushi
    • Publisher
      TRANS TECH PUBLICATIONS
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Experimental and Clinical Reconstructive Microsurgery2003

    • Author(s)
      H.Ohgushi, M.Akahane
    • Publisher
      Eds. Tamai, S., Springer-Verlag Tokyo
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Novartis Found Sympo.2492003

    • Author(s)
      Ohgushi H, Miyake J, Tateishi T.
    • Publisher
      John Wiley & Sons Ltd
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Biomaterials in Orthopedics2003

    • Author(s)
      Nishikawa, M., Ohgushi, H
    • Publisher
      Eds. Yaszemsk, MJ., Marcel Dekker Inc., New York, Basel
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Bioceramics142002

    • Author(s)
      M.Akahane, H.Ohgushi, S.Kuriyama, T.Akahane, K.Kawamura, Y.Takakura
    • Publisher
      TRANS TECH PUBLICATIONS
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Bioceramics142002

    • Author(s)
      H.Ohgushi, T.Noshi, M.Ikeuchi, T.Yoshikawa, Y.Dohi, T.Tateishi
    • Publisher
      TRANS TECH PUBLICATIONS
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Bioceramics2002

    • Author(s)
      H Ohgushi.
    • Publisher
      The McGraw-Hill Co., New York
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Recent Research Developments in Analytical Biochemistry2002

    • Author(s)
      Y.Dohi, H.Ohgushi, T.Minami, M Ikeuchi, K.Yonemasu
    • Publisher
      Scientific Research Flash, Vazhapalli India
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kotobuki, N., Hirose, M., Takakura Y., Ohgushi, H: "Cultured autologous human cells for hard tissue regeneration : preparation and characterization of mesenchymal stem cells from bone marrow."Artificial Organs. 28. 33-39 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kitamura, S., Ohgushi, H, Hirose, M., Funaoka, H., Takakura: "Osteogenic differentiation of human bone marrow-derived mesenchymal cells cultured on alumina ceramics."Artificial Organs. 28. 72-81 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shimaoka H., Dohi Y.Ohgushi H., Ikeuchi, M.: "Recombinant growth/differentiation factor-5 (GDF-5) stimulates osteogenic differentiation of marrow mesenchymal cells in porous hydroxyapatite ceramic"Journal of Biomedical Materials Researc. 68A2. 168-176 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kotobuki, N., Kawagoe, D., Fujimori, H., Ioku K., Ohgushi, H.: "In vitro osteogenic activity of rat bone marrow derived mesenchymal stem cells cultured on transparent hydroxyapatite ceramics"Key Engineering Materials (Bioceramics16). 254. 1055-1058 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirose, M., Kotobuki, N., Machida, H., Kitamura, S., Ohgushi, H.: "Osteogenic potential of cryopreserved human bone marrow-derived mesenchymal cells after thawing in culture"Materials Science and Engineering C. 24. 355-359 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kotobuki, N., Hirose, M., Funaoka, H., Ohgushi, H.: "in vitro osteoblastic potential after selective sorting of osteoblasts with high alkaline phosphatase activity from human osteoblast-like cells."Cell Transplantation,. In press.

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Ikeuchi, Y.Dohi, K.Horiuchi, H.Ohgushi, T.Noshi, T.Yoshikawa: "Recombinant Human Bone Morphogenetic Protein-2 Promotes Osteogenesis within Atelopeptide Type I Collagen Combination with Cultured Marrow Cells"J. Biomed. Mat. Res.. 60. 61-69 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Akahane, S.Kuriyama, Ohgushi, H.et al.: "Enhancing and suppressing effects of dexamethasone on transgene expression in vitro"International Journal of Molecular Medicine. 10. 107-112 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Akahane, H.Ohgushi, S.Kuriyama, et al.: "Hydroxyapatite ceramics as a cariier of gene-transduced bone marrow cells"J. Orthop. Scie.. 7. 677-682 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Ohgushi: "Bioceramics"McGraw-Hill Yearbook of Science & Technology (The McGraw-Hill Co., New York). 24-27 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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