| Project/Area Number |
14209009
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
広領域
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| Research Institution | Nagoya University |
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Principal Investigator |
WAKAMATSU Yuko Nagoya University, Bioscience and Biotechnology Center, Professor, 生物機能開発利用研究センター, 教授 (20026800)
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| Co-Investigator(Kenkyū-buntansha) |
SEIKI Makoto JST, SORST Project, Kondo Research Team, Team Leader, SORSTプロジェクト・近藤研究チーム, チームリーダー
HASHIMOTO Hisashi Nagoya University, Bioscience and Biotechnology Center, Assistant Professor, 生物機能開発利用研究センター, 助手 (30359757)
OZATO Kenjiro Nagoya University, Honorary Professor, 名誉教授
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥47,840,000 (Direct Cost: ¥36,800,000、Indirect Cost: ¥11,040,000)
Fiscal Year 2004: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2003: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2002: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
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| Keywords | mutagenesis / ENU / dominant mutation / recessive mutation / enlargement in the kidney / inversion in heart structure / enlargement in the heart / 透明メダカ / ミュータジェネシス / 変異体 / 内臓器官 |
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| Research Abstract |
The see-through medaka is transparent in the body wall and internal organs can be observed from outside in adult fish without dissection. To develop disease models, we carried out ENU mutagenesis in this medaka strain STII, to screen dominant and recessive mutations in adult internal organs.
Dominant mutations : Twenty-five candidates of dominant mutations were found in the F_1 generation. Nine showed enlargement or abnormal morphology in the heart, nine an enlargement morphology in the kidney, six They showed affecabnormality in the tissue color in the liver, and one popped eyes. Seventeen of them produced F_2 progenies in the crossing with STII. By histological studies carried out in the progenies with the affected phenotypes, one mutant showing an enlargement of the kidney tissues, two with an enlarged heart in addition to tumorous tissues in the kidney and liver, one with an enlarged heart, and one with an enlarged heart showing an abnormal position of the atrium and ventricle.
Recessive mutations : Six mutations were confirmed in the Fs generation, that is, three mutations with enlarged kidney, one which lacks an eye vesicle in the one side, and two showing abnormal laterality.
From these results, it became clear that the see-through medaka is a good material to screen mutations in internal organs in adult animals. Especially, the screening of mutations in the kidney in this medaka stock was effective because the tissue was easy to observe in the living fish and individuals with mutations in this organ generally can be survive for long time. The phenotype in the polycystic kidney disease in a medaka natural mutant, pc, was described to compare that in these mutations in kidney.
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