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Genetic analysis of the function and development of the inner ear pigment cells essential for hearing acuity.

Research Project

Project/Area Number 14340236
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionTohoku University

Principal Investigator

YAMAMOTO Hiroaki  Tohoku University, Graduate School of Life Sciences, Associate professor, 大学院・生命科学研究科, 助教授 (40174809)

Co-Investigator(Kenkyū-buntansha) GOJOBORI Takashi  National Institute of Genetics, Laboratory for Dna Data Analysis, Professor, 生命情報・DDBJ, 教授 (50162136)
IKEO Kazuho  National Institute of Genetics, Laboratory for Dna Data Analysis, Associate Professor, 生命情報・DDBJ, 助教授 (20249949)
YAMAMOTO Kazuo  Tohoku University, Graduate School of Life Sciences, Professor, 大学院・生命科学研究科, 教授 (20093536)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2004: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥8,100,000 (Direct Cost: ¥8,100,000)
Keywordsmouse / coat color mutant / pigment cell / Hearing / cochlea / stria vascularis / in situ hybridization / hearing loss / 内耳色素細胞 / サブトラクション / in situハイブリダイゼイション
Research Abstract

Little is known about the molecular mechanisms by which melanocytes, developed from neural crest cells, play an essential role in hearing acuity. The aim of this study is to elucidate the gene expression profile of the mouse inner ear affected by differentiation of the inner ear melanocytes and to infer the evolution of this melanocyte-envolved inner ear system. First, we found that inner ear melanocytes produce black melanin irrelevant to the coat color of mice. Next, we made two cDNA libraries prepared from the stria vascularis and its surrounding area of the inner ear obtained from wild type mouse and Mitf^<mi-bw> mutant mouse that shows aberrant pigmentation and hearing loss due to the failure of melanocyte differentiation. Then we obtained a subtraction library between those libraries. Differential screening was also carried out using this subtracted library. Micro array analysis of gene expression in each strain was also performed. Expression of many clones that showed differential expression in these two mouse strains was analyzed using in situ hybridization. Strangely enough, many of clones did not show significant in situ signals in each strain, nor many other clones showed prominent differences in expression between these two strains. Still, there were several clones express differentially and some of them did not express in the melanocytes but in their surrounding tissues. Although we need to analyze the sequence structure of these clones in detail, one putative gene seems to be involved in. stress reaction in melanocytes and another one seems to share a conserved roll with ascidian brain pigment cells that play as sensory organs. Functional analysis of these genes should be very important in future.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (17 results)

All 2004 2003 Other

All Journal Article (9 results) Book (3 results) Publications (5 results)

  • [Journal Article] Pigment cell lineage-specific expression activity of the ascidian tyrosinase-related gene.2004

    • Author(s)
      Toyoda, R., -Ikeo, K., Gojobori, T., -Yamamoto, H.
    • Journal Title

      Gene 332

      Pages: 61-69

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isolation and developmental expression of Mitf in Xenopus laevis2004

    • Author(s)
      Kumasaka, M., Sato, H., Sato, S., Yajima, I., Yamamoto, H.
    • Journal Title

      Dev.Dyn. 230

      Pages: 107-113

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pigment cell lineage-specific expression activity of the ascidian tyrosinase-related gene.2004

    • Author(s)
      Toyoda, R., Kasai, A., Sato, S., Wada, S., Saiga, H., Ikeo, K., Gojobori, T., Numakunai, T., Yamamoto, H.
    • Journal Title

      Gene 332

      Pages: 61-69

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isolation and developmental expression of Mitf in Xenopus laevis.2004

    • Author(s)
      Kumasaka, M., Sato, H., Sato, S., Yajima, I., Yamamoto, H.
    • Journal Title

      Dev.Dyn. 230

      Pages: 107-113

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pigment cell lineage-specific expression activity of the ascidian tyrosinase-related gene.2004

    • Author(s)
      Toyoda, R., ---Ikeo, K., Gojobori, T., ---Yamamoto, H.
    • Journal Title

      Gene 332

      Pages: 61-69

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cloning and functional analysis of ascidian Mitf in vivo : insights into the origin of vertebrate pigment cells.2003

    • Author(s)
      Yajima I., ---Ikeo K., Gojobori T., Goding C.R., Yamamoto H.
    • Journal Title

      Mech.Dev. 120

      Pages: 1489-1504

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isolation and developmental expression of tyrosinase family genes in Xenopus laevis2003

    • Author(s)
      Kumasaka, M., SATO, S., Yajima, I, Yamamoto, H
    • Journal Title

      Pigment Cell Research 16

      Pages: 455-462

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cloning and functional analysis of ascidian Mitf in vivo : insights into the origin of vertebrate pigment cells.2003

    • Author(s)
      Yajima I., Endo K., Sato S., Toyoda R., Wada H., Shibahara S., Numakunai T., Ikeo K., Gojobori T., Goding C.R., Yamamoto H.
    • Journal Title

      Mech.Dev. 120

      Pages: 1489-1504

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isolation and developmental expression of tyrosinase family genes in Xenopus laevis2003

    • Author(s)
      Kumasaka, M., SATO, S., Yajima, I, Yamamoto, H.
    • Journal Title

      Pigment Cell Research 16

      Pages: 455-462

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] コートカラー「モデル動物の作製と維持」pp498-523(森脇和郎,山村研一,米川博通 共編)2004

    • Author(s)
      山本 博章
    • Publisher
      LIC(Life-Science Information Center),東京
    • Related Report
      2004 Annual Research Report
  • [Book] 脊椎動物色素細胞と進化「ゲノムから見た生物の多様性と進化」(五條掘孝編)2003

    • Author(s)
      山本博章
    • Total Pages
      222
    • Publisher
      シュプリンガー・フェアラーク東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Evolution of vertebrate pigment cells in Genomics Unveils Diversity and Evolution(T.Gojobori)(Tokyo)2003

    • Author(s)
      Yamamoto, H.
    • Total Pages
      222
    • Publisher
      Springer-Verlag Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 山本 博章: "Isolation and developmental expression of tyrosinase family genes in Xenopuslaevis"Pigment Cell Research. 16. 455-462 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山本博章, 池尾一穂, 五條掘孝: "Cloning and functional analysis of ascidian Mitf in vivo : insights into the origin of vertebrate pigment cells"Mechanisms of Develpment. 120. 1489-1504 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山本博章: "Isolation and developmental expression of Mitf in Xenopus laevis"Developmental Dynamics. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] 山本博章: "五條堀孝編 ゲノムから見た生物の多様性と進化"シュプリンガー・フェアラーク東京. 222 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山本 博章: "色素細胞発生機構の進化-多細胞生物の発生機構の進化を探るモデルシステムとして-"学術月報. 55. 1153-1159 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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