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Analysis of molecular mechanism of action of flowering-time genes in Arabidopsis thaliana

Research Project

Project/Area Number 14340254
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionKyoto University

Principal Investigator

ARAKI Takashi  Kyoto University, Department of Botany, Graduate School of Science, Associate Professor, 大学院・理学研究科, 助教授 (00273433)

Co-Investigator(Kenkyū-buntansha) ABE Mitsutomo  Kyoto University, Department of Botany, Graduate School of Science, Assistant Professor, 大学院・理学研究科, 助手 (20343238)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥11,100,000 (Direct Cost: ¥11,100,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2003: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsHigher plants / Growth phase transition / Flowering / Photoperiodism / Floral morphogenesis / Signal transduction / Transcription factor / Arabidopsis / 成長相変換 / 花成遺伝子 / 花成遅延変異体 / 転写因子 / 転写メディエータ
Research Abstract

In the long-day plant, Arabidopsis, environmental and endogenous signals transduced by several pathways are integrated at the transcriptional regulation of so-called "floral pathway integrators". FLOWERING LOCUS T(FI) is one of the floral pathway integrators, and elucidation of molecular mechanism of action of FT gene product is an important step toward understanding of regulation of flowering. With this in mind, we investigated molecular mechanism of the FT action through analysis of two genes. One is a flowering-time gene FD, whose function is required for FT to promote flowering. The other is TWIN SISTER OF FT (TSF) which is a closest homelog of FT in Arabidopsis.
We identified the FD gene by a map-based approach. The FD gene encodes a bZIP transcription factor preferentially expressed in the shoot apex. We showed that a floral meristem identity gene APETALA1 (AP1) which acts at the earliest step in floral fate specification is a regulatory target of FD and that FD requires the FT fu … More nction to activate AP1 transcription. Mutual dependence between FT and FD is mediated by protein-protein interaction. which we demonstrated by yeast two-hybrid assay, in vitro pull-down assay, and bimolecular fluorescent complementation assay in plant cells. Expression of FT in either vasculature, which is the site of expression, or shoot apex could rescue ft mutants. By contrast, FD expressed in the shoot apex, but not in vasculature was effective to rescue fd mutants. These results and mutual dependence of FT and FD via protein interaction strongly suggest that the site of action of FT is shoot apex where it may modulate the activity of FD. This led us to propose that FT may represent a long-sought long distance signal in flowering, called florigen (Abe et al., Science, in press).
We investigated the role of TSF in regulation flowering. Previous work has suggested a potential role of TSF in promotion of flowering. We showed that TSF and FT share similar modes of regulation : direct positive regulation by CONSTANS, diurnal oscillation with an evening peak, response to photoperiod, negative regulation by FLOWERING LOCUS C, and vernalization response. TSF and FT also share regulation by EARLY BLTING IN SHORT DAYS and phyB. tsf enhanced ft phenotype in both long-day and short-day conditions. These strongly suggest that TSF is a floral pathway integrator acting redundantly with FT. TSF and FT share preferential expression in phloem tissues, which led us to propose that the phloem represents on of the sites of pathway integration (Yamaguchi et al., Plant & Cell Physiology, in press).
A current hypothesis from our work is : FT and TSF integrate signals from various pathways in-phloem tissues and their gene products (possibly proteins) are somehow transported to the shoot apex where they modulate the activity of a bZIP transcription factor FD which in turn activate AP! transcription and initiate floral morphogenesis. To test this is an important step forward in understanding flowering. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (28 results)

All 2005 2004 2003 2002 Other

All Journal Article (14 results) Book (7 results) Publications (7 results)

  • [Journal Article] Phytochrome B in the mesophyll delays flowering by suppressing FLOWERING LOCUS T expression in Arabidopsis vascular bundles.2005

    • Author(s)
      Motomu Endo, Satoshi Nakamura, Takashi Araki, Nobuyoshi Mochizuki, Akira Nagatani
    • Journal Title

      Plant Cell 17

      Pages: 1941-1952

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] TWIN SISTER OF FT(TSF) acts as a floral pathway integrator redundantly with FT.2005

    • Author(s)
      Ayako Yamaguchi, Yasushi Kobayashi, Koji Goto, Mitsutomo Abe, Takashi Araki
    • Journal Title

      Plant & Cell Physiology 46

      Pages: 1175-1189

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FD, A bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex.2005

    • Author(s)
      Mitsumoto Abe, Yasushi Kobayashi, Sumiko Yamamoto, Yufumi Daimon, Ayako Yamaguchi, Yoko Ikeda, Harutaka Ichinoki, Michitaka Notaguchi, Koji Goto, Takashi Araki
    • Journal Title

      Science 309

      Pages: 1052-1056

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ectopic expression of an FT homolog from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliate L. Raf.)2005

    • Author(s)
      Tomoko Endo, Takehiko Shimada, Hiroshi Fujii, Yasushi Kobayashi, Takashi Araki, Mitsuo Omura
    • Journal Title

      Transgenic Research 14(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phytochrome B in the mesophyll delays flowering by suppressing FLOWERING LOCUS T expression in Arabidopsis vascular bundles.2005

    • Author(s)
      Motomu ENDO, Satoshi NAKAMURA, Takashi ARAKI, Nobuyoshi MOCHIZUKI, Akira NAGATANI
    • Journal Title

      Plant Cell 17(7)

      Pages: 1941-1952

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] TWIN SISTER OF ET (TSF) acts as a floral pathway integrator redundantly with FT.2005

    • Author(s)
      Ayako YAMAGUCHI, Yasushi KOBAYASHI, Koji GOTO, Mitsutomo ABE, Takashi ARAKI
    • Journal Title

      Plant and Cell Physiology 46(8)(Cover of the issue)

      Pages: 1175-1189

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex.2005

    • Author(s)
      Mitsutomo ABE, Yasushi KOBAYASHI, Sumiko YAMAMOTO, Yasufumi DAIMON, Ayako YAMAGUCHI, Yoko IKEDA, Harutaka ICHINOKI, Michitaka NOTAGUCHI, Koji GOTO, Takashi ARAKI
    • Journal Title

      Science 309(5737)

      Pages: 1052-1056

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ectopic expression of an FT homolog from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L.Raf.)2005

    • Author(s)
      Tomoko ENDO, Takehiko SHIMADA, Hiroshi FUJII, Yasushi KOBAYASHI, Takashi ARAKI, Mitsuo OMURA
    • Journal Title

      Transgenic Research 14(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] BRU1, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis.2004

    • Author(s)
      Shin Takeda, Zerihun Tadele, Ingo Hofmann, Aline V.Probst, Karel J.Agelis, Hidetaka Kaya, Takashi Araki, Tesfaye Mengiste, Ortrun Mittelsten Scheid, Kei-ichi Shibahara, Dierk Scheel, Jerzy Paszkowski
    • Journal Title

      Genes & Development 18

      Pages: 782-793

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] BRU1, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis.2004

    • Author(s)
      Shin TAKEDA, Aerihun TADELE, Ingo HOFMANN, Aline V.PROBST, Karel J.ANGELIS, Hidetaka KAYA, Takashi ARAKI, Tesfaye MENGISTE, Ortrun MMITELSTEN SCHEID, Kei-ichi SHIBAHARA, Dierk SCHEEL, Jerzy PASZKOWSKI
    • Journal Title

      Gene and Development 18(7)

      Pages: 782-793

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] BRU1,a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis2004

    • Author(s)
      Takeda, Shin
    • Journal Title

      Genes and Development 18・7

      Pages: 782-793

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day condition.2002

    • Author(s)
      Shoko Kojima, Yuji Takahashi, Yasushi Kobayashi, Lisa Monna, Takuji Sasaki, Takashi Araki, Masahiro Yano
    • Journal Title

      Plant & Cell Physiology 43

      Pages: 1096-1105

    • NAID

      10010381544

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 長日植物における花成制御2002

    • Author(s)
      荒木 崇
    • Journal Title

      蛋白質 核酸 酵素 47

      Pages: 1535-1540

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hd3α, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day condition.2002

    • Author(s)
      Shoko KOJIMA, Yuji TAKAHASHI, Yasushi KOBAYASHI, Lisa MONNA, Takuji SASAKI, Takashi ARAKI, Masahiro YANO
    • Journal Title

      Plant and Cell Physiology 42(10)(Cover of the issue JSPP Plant and Cell Physiology Award for the Paper of Excellence)

      Pages: 1096-1105

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 改訂3版 モデル植物の実験プロトコール(島本 功, 岡田清孝, 田畑哲之(監修))2005

    • Author(s)
      大門靖, 阿部光知, 荒木 崇(分担執筆)
    • Publisher
      秀潤社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] モデル植物の実験ブロトコール(分担執筆)2005

    • Author(s)
      岡田清孝, 島本功(監修)
    • Publisher
      秀潤社(印刷中)
    • Related Report
      2004 Annual Research Report
  • [Book] 植物の環境応答と形態形成のクロストーク(岡 穆宏, 岡田清孝, 篠崎一雄(編))2004

    • Author(s)
      荒木 崇(分担執筆)
    • Publisher
      シュプリンガー・フェアラーク東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Taiz & Zeiger 植物生理学 第三版(島崎研一郎, 西谷和彦(監訳))2004

    • Author(s)
      荒木 崇(分担翻訳)
    • Publisher
      培風館
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 植物の環境応答と形態形成のクロストーク(分担執筆)2004

    • Author(s)
      岡 穆宏 他(編)
    • Total Pages
      230
    • Publisher
      シュプリンガー・フェアラーク東京
    • Related Report
      2004 Annual Research Report
  • [Book] タイツ・植物生理学(分担翻訳)2004

    • Author(s)
      西谷和彦, 島崎研一郎(監訳)
    • Total Pages
      679
    • Publisher
      培風館
    • Related Report
      2004 Annual Research Report
  • [Book] 光合成事典(日本光合成研究会(編))2003

    • Author(s)
      荒木 崇(分担執筆)
    • Total Pages
      430
    • Publisher
      学会出版センター
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Takeda, Shin: "BRUJ, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis"Genes and Development. 18・7(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 岡 穆宏 他(編): "植物の環境応答と形態形成のクロストーク(分担執筆)"シュプリンガー・フェアラーク東京. 230 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 日本光合成研究会(編): "光合成事典(分担執筆)"学会出版センター. 430 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 西谷和彦, 島崎研一郎(監訳): "タイツ・植物生理学(分担翻訳)"培風館(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kojima, Shoko(小島晶子): "Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day condition"Plant&CellPhysiology. 43巻10号. 1096-1105 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 荒木 崇: "長日植物における花成制御理解の現状と未解決の課題"蛋白質核酸酵素. 43巻8号. 1535-1540 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 日本光合成研究会(編): "光合成事典(分担執筆)"学会出版センター(未定 印刷中). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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