| Project/Area Number |
14340255
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | University of Hyogo (2004)
Himeji Institute of Technology (2002-2003) |
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Principal Investigator |
SHIMMEN Teruo University of Hyogo, Graduate School of Life Science, Professor, 大学院・生命理学研究科, 教授 (80114510)
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| Co-Investigator(Kenkyū-buntansha) |
SONOBE Seiji University of Hyogo, Graduate School of Life Science, Associate professor, 大学院・生命理学研究科, 助教授 (30197024)
YOKOTA Etsuko University of Hyogo, Graduate School of Life Science, Assistant professor, 大学院・生命理学研究科, 助手 (80212299)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 2004: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 2003: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2002: ¥6,900,000 (Direct Cost: ¥6,900,000)
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| Keywords | actin / myosin / Arabidopsis / peroxisome / stomata cell / root hair cell / cytoplasmic streaming / MYA2 / 培養細胞 / プロセッシビティー / 花粉管 / リン酸化 / ビリン / タバコ培養細胞 / 脱リン酸化 / カリキュリンA |
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| Research Abstract |
Cytoplasmic streaming is generally observed in plant cells and involved in intracellular transport. It has been suggested that actin-myosin system is involved in generation of the motive force of cytoplasmic streaming. We had succeeded in isolating myosin molecules involved in cytoplasmic streaming in tobacco cultured cells and lily pollen tubes. In Arabidopsis thaliana, two kinds of genes supposed to code myosin molecule had been reported (myosin VIII and myosin XI). However, the function of had been remained to be elucidated. It was supposed that myosin XI is involved in cytoplasmic streaming. It has been suggested that the N terminal region has a function to slide along actin filaments and that C terminal region has a function to bind organelles. In the present study, we tried to identify the organlles carried by mysin XI.
In Arabidopsis thaliana, three classes of myosin XI gene have been identified. We raised an antibody against C terminal region of MYA2 myosin. Immunocytochemical analysis revealed that this myosin is co-localized with actin filaments in epidermal cells of leaf, root hair cells and cultured cells. Distribution of this myosin in stomatal cell suggested that this myosin may associated with peroxisomes. It has been suggested that actin filament is concerned with intracellular transport of peroxisomes. However, the moter protein was remained to be identified. Therefore, we carried out an analysis using transgenic Arabidopsis plants expressing GFP in peroxisomes. Itr was found that this myosin is really associated with peroxisomes, and suggested that this myosin is responsible for transport of peroxisomes.
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