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Molecular mechanisms for recognition and transport of proteins by a protein complex exists in peroxisomal membrane

Research Project

Project/Area Number 14340256
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionOkazaki National Research Institutes

Principal Investigator

HAYASHI Makoto  Okazaki National Research Institutes, National institute for Basic Biology, Department of Cell Biology, Associate professor, 基礎生物学研究所, 助教授 (50212155)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2003: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥11,300,000 (Direct Cost: ¥11,300,000)
Keywordsperoxisome / protein transport / lipid degradation / photorespiration / β-oxidation / glyoxysome / peroxin
Research Abstract

Peroxisomal proteins are transported from the cytosol into peroxisomes by a recognition of one of the two protein targeting signals called PTS1 and PTS2. Last year, we identified two receptors (Pex5p and Pex7p) that can capture PTS 1 and PTS2, and showed protein-protein interaction between Pex5p and Pex7p, as well as Pex5p and a peroxisomal membrane protein, Pex14p. In this fiscal year, we produced transgenic plants that suppressed PEX5-and PEX7-gene expression by RNA interference technology. Analyses of these transgenic plants showed following 2 points. (1) Loss of Pex5p inhibits the import of both PTS1-and PTS2-containing proteins into peroxisomes. The transgenic plants without Pex5p showed defects in functions of both glyoxysomes and leaf peroxisomes. (2) Loss of Pex7p inhibits the import of only PTS2-containing proteins. The transgenic plants without Pex7p showed defect only in glyoxysomal function. From these observation, we concluded that Pex5p and Pex7p form a receptor-cargo complex in the cytosol, and the receptor-cargo complex is captured by the protein-protein interaction between Pex5p and the peroxisomal membrane protein, Pex14p.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Kamada, T., et al.: "Monitoring expression profiles of 281 peroxisomal genes revealed functional diversity of plant peroxisomes"Plant Cell Physiol.. 44. 1275-1289 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukao, Y., et al.: "Novel glyoxysomal protein kinase, GPK1, identified by proteomic analysis of glyoxysomes in etiolated cotyledons of Arabidopsis thaliana"Plant Cell Physiol.. 44. 1002-1012 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 二藤和昌ら: "植物ペルオキシソームの機能分化と生合成機構"実験医学増刊細胞内輸送研究の最前線. 21. 1917-1923 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hayashi, M., et al.: "Entering a new era of research on plant peroxisomes"Cur.Opin.Plant Sci.. 6. 577-582 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 林 誠: "光合成事典(ペルオキシソーム関連各項目)"学会出版センター. 430 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamada, T., et al.: "Monitoring expression profiles of 281 peroxisomal genes revealed functional diversity of plant peroxisomes"Plant Cell Physiol.. 44. 1275-1289 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukao, Y., et al.: "Novel glyoxysomal protein kinase, GPK1, identified by proteomic analysis of glyoxysomes in etiolated cotyledons of Arabidopsis thaliana"Plant Cell Physiol. 44. 1002-1012 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hayashi, M., et al.: "Entering a new era of research on plant peroxisomes"Cur.Opin.Plant Sci.. 6. 577-582 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamada, T., et al.: "Monitoring expression profiles of 281 peroxisomal genes revealed functional diversity of plant peroxisomes"Plant Cell Physiol.. 44. 1275-1289 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fukao, Y., et al.: "Novel glyoxysomal protein kinase, GPK1, identified by proteomic analysis of glyoxysomes in etiolated cotyledons of Arabidopsis thaliana"Plant Cell Physiol.. 44. 1002-1012 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 二藤和昌ら: "植物ペルオキシソームの機能分化と生合成機構"実験医学 増刊 細胞内輸送研究の最前線. 21. 1917-1923 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hayashi, M., et al.: "Entering a new era of research on plant peroxisomes"Cur.Opin.Plant Sci.. 6. 577-582 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 林 誠: "光合成事典(ペルオキシソーム関連各項目)"学会出版センター. 430 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kamigaki et al.: "Identification of peroxisomal targeting signal of pumpkin catalase and the binding analysis with PTS1 receptor"Plant J.. 33. 161-175 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hayashi et al.: "Molecular characterization of an Arabidopsis acyl CoA synthetase localized on glyoxysomal membranes"Plant Physiol.. 130. 2019-2026 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fukao et al.: "Proteomic analysis of leaf peroxisomal proteins in green cotyledons of Arabidopsis thaliana"Plant Cell Physiol.. 43. 689-696 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nito et al.: "Direct interaction and determination of binding domains among peroxisomal import factors in Arabidopsis thaliana"Plant Cell Physiol.. 43. 355-366 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mano et al.: "Distribution and characterization of peroxisomes in Arabidopsis by visualization with GFP : dynamic morphology and actin-dependent movement"Plant Cell Physiol.. 43. 331-341 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hayashi et al.: "Ped3p is a peroxisomal ATP-binding cassette transporter that might supply substrates for fatty acid β-oxidation"Plant Cell Physiol.. 43. 1-11 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 林 誠, 西村幹夫: "脂肪性種子植物における脂肪酸分解の欠損とその影響"バイオサイエンスとインダストリー. 60. 9-10 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 二藤和昌: "ペルオキシソームの分化"細胞工学別冊植物細胞工学シリーズ17 植物オルガネラの分化と多様性. 17. 139-149 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山谷知行: "ゲノム科学と21世紀の植物栄養学-具体例の紹介-"日本土壌肥料学会雑誌. 73. 69-72 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 林 誠, 西村幹夫: "朝倉植物生理学講座第1巻植物細胞 4.4ペルオキシソーム"朝倉書店. 96-110 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 林 誠, 西村幹夫: "生物学データ大百科事典 1-4-1 e.ミクロボディー"朝倉書店. 120-126 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hayashi, M., Nishimura, M.: "Plant peroxisome Genetic approaches to understand plant peroxisomes"Kluwer Academic Publishers. 279-303 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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