Functions and Mechanisms of Action of the Medaka Sex-Determining Gene
| Project/Area Number |
14340259
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物形態・構造
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| Research Institution | Okazaki National Research Institutes, National Institute for Basic Biology |
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Principal Investigator |
NAGAHAMA Yoshitaka National Institute for Basic Biology, Developmental Biology, Professor, 基礎生物学研究所, 教授 (50113428)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2003: ¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2002: ¥7,700,000 (Direct Cost: ¥7,700,000)
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| Keywords | Fish / Sex-determining gene / Testis / Sertoli cell / Sex differentiation / Medaka / DMY / DMRT1 / 性決定 / 精巣分化 / Y染色体 / 脊椎動物 / 特異抗体 |
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| Research Abstract |
We previously identified PG17 as the leading candidate for the medaka sex-determining gene. To definitively identify PG17 as a sex-determining gene, we injected one-cell stage medaka embryos with a genomic DNA fragment carrying PG17. The transgenic embryos were bred until secondary sexual characters were observed. We found that all XX transgenic fish were male, showing that PG17 is sufficient to induce male development. The present data combined with our previous data indicate that PG17 is the sex-determining gene of medaka and named it DMY.
DMY mRNA and protein are expressed specifically in the somatic cells (Sertoli cells) surrounding primordial germ cells (PGC) in the early gonadal primordium, before morphological sex differences are apparent. DMRT1, another DM domain gene that is also involved in male development in other vertebrates, is expressed in spermatogonium-supporting cells after testicular differentiation (20-30 days after hatching). These results strongly suggest that DMY regulates PGC proliferation and differentiation sex-specifically during early gonadal differentiation of XY individuals and that DMRT1 regulates spermatogonial differentiation.
Because Sry transgenic mice are sterile and because DMY transgenic XX medakas are fertile, i.e., fully functional males, DMY transgenic medakas are the first transgenic vertebrates known to undergo complete sex reversal.
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Report
(3 results)
Research Products
(27 results)
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[Journal Article] Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes.2004
Author(s)
Kobayashi, T., Matsuda, M., Kajiura-Kobayashi, H., Suzuki, A., Saito, N., Nakamoto, M., Shibata, N., Nagahama, Y.
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Journal Title
Dev.Dyn. 231
Pages: 518-526
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Oryzias curvinotus has DMY, a gene that is required for male development in the medaka2003
Author(s)
Matsuda, M., Sato, T., Toyazaki, Y., Nagahama, Y., Hamaguchi, S., Sakaizumi, M.
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Journal Title
O.latipes.Zool.Sci. 20
Pages: 159-161
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Sox9 in a teleost fish, medaka (Oryzias latipes) : Evidence for diversified function of Sox9 in gonad differentiation.2002
Author(s)
Yokoi, H., Kobayashi, T., Tanaka, M., Nagahama, Y., Wakamatsu, Y., Takeda, H., Araki, K., Morohashi, K., Ozato, K.
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Journal Title
Mol.Reprod.Develop. 63
Pages: 5-16
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] DMY is a Y-specific DM-domain gene required for male development in the medaka fish.2002
Author(s)
Matsuda, M., Nagahama, Y., Shinomiya, A., Sato, T., Matsuda, C., Kobayashi, T., Morrey, C.E., Shibata, N., Asakawa, S., Shimizu, N., Hori, H., Hamaguchi, S., Sakaizumi, M.
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Journal Title
Nature 417
Pages: 559-563
Description
「研究成果報告書概要(欧文)」より
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