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Physiological analyzes of synaptic mechanisms underlying the central compensation in crayfish using simultaneous intracellular recording techniques

Research Project

Project/Area Number 14340260
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

TAKAHATA Masakazu  Hokkaido Univ., Grad.School of Sci., Prof, 大学院・理学研究科, 教授 (10111147)

Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥9,100,000 (Direct Cost: ¥9,100,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2003: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥3,700,000 (Direct Cost: ¥3,700,000)
KeywordsCrustaceans / Statocyst / Postural control / Central compensation / Nonspiking giant interneurons / Local interneurons / Crayfish / Simultaneous Intracellular Recording / 局在介在ニューロン / 細胞内記録・染色法 / NGI / 前大脳 / 中大脳 / シナプス接続 / 甲殻類 / 樹状突起 / シナプス
Research Abstract

When the equilibrium organ of crustaceans is partially disabled, the eyestalks first take bilaterally asymmetrical posture, but they gradually recover the original symmetrical posture in a few weeks. Although this recovery process has long been known as the central compensation at the behavioral level, the physiological process subserving it largely remains unknown at the neuronal level. In this study, the synaptic mechanisms underlying the central compensation of the eyestalk posture following unilateral statolith removal have been investigated using intracellular recording and staining techniques in crayfish. Physiological analyzes of the dendritic membrane properties and synaptic activities of nonspiking giant interneurons (NGIs) that play a crucial role in the sensori-motor signal transmission for controlling the eyestalk posture revealed that not only their passive membrane properties but also the spontaneous synaptic activities showed significant changes during the compensation p … More rocess. The finding that the input resistance and membrane time constant increased or decreased after unilateral statolith removal, depending on the side of operation, clearly demonstrated that NGIs are the site of changes leading to the central compensation. However, since the spontaneous synaptic activity of NGIs reflects the spike activity of those cells that are presynaptic to NGIs, it was suggested that those presynaptic interneurons also play important roles in the central compensation. Many local interneurons that made either monosynaptic or polysynaptic connection with NGIs were identified by simultaneous intracellular recording from them. Most of these local interneurons projected dendritic branches onto the parolfactory lobe of the deutocerebrum where statocyst afferents also project directly as well as onto the protocerebrum where dendrites of NGIs are located, thus mediating statocyst information to NGIs. It is concluded that the central compensation process is based on activity changes at multiple sites in the brain including NGIs and their presynaptic cells identified in this study. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (27 results)

All 2005 2004 2003 2002 Other

All Journal Article (21 results) Publications (6 results)

  • [Journal Article] 無脊椎動物における非拘束条件下での生体信号の記録:光テレメトリシステム2005

    • Author(s)
      濱徳行
    • Journal Title

      生物物理 (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Modification of statocyst input to local interneurons by behavioral condition in the crayfish brain2005

    • Author(s)
      N.Hama
    • Journal Title

      J.Comp.Physiol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 無脊椎動物における非拘束条件下での生体信号の記録:光テレメトリシステム2005

    • Author(s)
      濱徳行
    • Journal Title

      生物物理 印刷中

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Further exploration into the adaptive design of the arthropod "microbrain": I. Sensory and memory-processing systems.2004

    • Author(s)
      Mizunami, M.
    • Journal Title

      Zool.Sci. 21

      Pages: 1141-1151

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 光テレメトリシステムを用いた水中での非拘束動物からの生体信号の記録2004

    • Author(s)
      土田義和
    • Journal Title

      比較生理生化学 21

      Pages: 100-105

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Characterization of electro-olfactogram oscillations and their computational reconstruction.2004

    • Author(s)
      Suzuki, N.
    • Journal Title

      Chem.Senses 29

      Pages: 411-424

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An optical telemetry system for underwater recording of electromyogram and neuronal activity from non-tethered crayfish.2004

    • Author(s)
      Tsuchida, Y.
    • Journal Title

      J.Neurosci.Methods 137

      Pages: 103-109

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Further exploration into the adaptive design of the arthropod "microbrain" : I.Sensory and memory-processing systems2004

    • Author(s)
      M.Mizunami
    • Journal Title

      Zool.Sci. 21

      Pages: 1141-1151

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of electro-olfactogram oscillations and their computational reconstruction2004

    • Author(s)
      N.Suzuki
    • Journal Title

      Chemical Senses 29

      Pages: 411-424

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An optical telemetry system for underwater recording of electromyogram and neuronal activity from non-tethered crayfish2004

    • Author(s)
      Y.Tsuchida
    • Journal Title

      J.Neurosci.Methods 137

      Pages: 103-109

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An optical telemetry system for underwater recording of electromyogram and neuronal activity from non-tethered crayfish2004

    • Author(s)
      Tsucbida, Y.
    • Journal Title

      J.Neurosci.Methods 137

      Pages: 103-109

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of electro-olfactogram oscillations and their computational reconstruction2004

    • Author(s)
      Suzuki, N.
    • Journal Title

      Chemical Senses 29

      Pages: 411-424

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Further exploration into the adaptive design of the arthropod "microbrain" : I.Sensory and memory-processing systems2004

    • Author(s)
      Mizunami, M.
    • Journal Title

      Zool.Sci. 21

      Pages: 1141-1151

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of leg movements on the synaptic activity of descending statocyst interneurons in crayfish, Procambarus clarkii.2003

    • Author(s)
      N.Hama
    • Journal Title

      J.Comp.Physiol. 189

      Pages: 877-888

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mechanoreceptors involved in the hindwing-evoked escape behaviour in cricket Gryllus bimaculatus.2003

    • Author(s)
      Hiraguchi, T.
    • Journal Title

      J.Exp.Biol. 206

      Pages: 523-534

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of leg movements on the synaptic activity of descending statocyst interneurons in crayfish, Procambarus clarkii2003

    • Author(s)
      N.Hama
    • Journal Title

      J.Comp.Physiol. 189

      Pages: 877-888

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mechanoreceptors involved in the hindwing-evoked escape behavior in cricket, Gryllus bimaculatus2003

    • Author(s)
      T.Hiraguchi
    • Journal Title

      J.Exp.Biol. 206

      Pages: 523-534

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Experimental modification of stepping course in spontaneously initiated locomotor behavior in the crayfish Procambarus clarkii Girard.2002

    • Author(s)
      Yamane, S.
    • Journal Title

      J.Comp.Physiol. 188

      Pages: 13-23

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oscillatory current responses of olfactory receptor neurons to odorants and computer simulation based on a cyclic AMP transduction model.2002

    • Author(s)
      Suzuki, N.
    • Journal Title

      Chem.Senses 27

      Pages: 789-801

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oscillatory current responses of olfactory receptor neurons to odorants and computer simulation based on a cyclic AMP transduction model2002

    • Author(s)
      N.Suzuki
    • Journal Title

      Chemical Senses 27

      Pages: 789-801

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Experimental modification of stepping course in spontaneously initiated locomotor behavior in the crayfish Procambarus clarkii Girard2002

    • Author(s)
      S.Yamane
    • Journal Title

      J.Comp.Physiol. 188

      Pages: 13-23

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Hiraguchi, T., Yamaguchi, T., Takahata, M.: "Mechanoreceptors involved in the hindwing-evoked escape behaviour in cricket, Gryllus bimaculatus"Journal of Experimental Biology. 206. 523-534 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] N.Hama, M.Takahata: "Effects of leg movements on the synaptic activity of descending statocyst interneurons in crayfish, Procambarus clarkii"Journal of Comparative Physiology. 189. 877-888 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsuchida, Y., Hama, N., Takahata, M.: "An optical telemetry system for underwater recording of electromyogram and neuronal activity from non-tethered crayfish"Journal of Neuroscience Methods. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamane, S., Takahata, M: "Experimental modification of stepping course in spontaneously initiated locomotor behavior in the crayfish Procambarus clarkii Girard"Journal of Comparative Physiology. 188. 13-23 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki, N., Takahata, M., Sato, K.: "Oscillatory current responses of olfactory receptor neurons to odorants and computer simulation based on a cyclic AMP transduction model"Chemical Senses. 27. 789-801 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fraser, P.J., Takahata, M.: "Statocysts and statocyst control of motor pathways in crayfish and crabs. In : "Crustacean Experimental Systems in Neurobiology""Springer/Berlin. 89-108 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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