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Demonstration of multiforms of insect cytokine receptors

Research Project

Project/Area Number 14340261
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionSaga University (2004-2005)
Hokkaido University (2002-2003)

Principal Investigator

HAYAKAWA Yoichi  Saga University, Faculty of Agriculture, Professor, 農学部, 教授 (50164926)

Co-Investigator(Kenkyū-buntansha) SHIMADA Kimio  Hokkaido University, Institute of Low Temperature Science, Research associate, 低温科学研究所, 助手 (30001663)
AIZAWA Tomoyasu  Hokkaido University, Graduate School of Science, Research associate, 大学院・理学研究科, 助手 (40333596)
Project Period (FY) 2002 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥16,600,000 (Direct Cost: ¥16,600,000)
Fiscal Year 2005: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2002: ¥5,500,000 (Direct Cost: ¥5,500,000)
Keywordsinsect / cytokine / growth-blocking peptide / receptor / cell membrane protein / tyrosine phosphorylation / intracellular signal transduction / peptide / growth-blocking peptide (GBP) / GBPレセプター / 血球細胞 / シグナル伝達 / 血球 / 膜タンパク質 / アワヨトウ / HighFive / GBP(growth-blocking peptide) / アフィニティークロマトグラフィー / 精製 / 細胞増殖 / 血球活性化 / GBP(growth blocking peptide)
Research Abstract

Growth-blocking peptide (GBP) is a member of an insect cytokine family. GBP is a small peptide consists of 25 amino acid residues, but it exerts diverse functions including larval growth retardation, cell proliferation, paralysis induction, cardioacceleration, immune cell stimulation, and morphogenesis. In this study, deletion analogs of GBP were synthesized to investigate the relationship between the structure of GBP and the biological activities such as mitogenic and plasmatocyte (a type of hemocytes) spreading activities. The results indicated that the minimal structure of GBP containing mitogenic activity is 2-23 GBP, whereas that with plasmatocyte spreading activity is 1-22 GBP, indicating the possibility that multifunctional properties of this peptide may be mediated by different forms of GBP receptors. In order to demonstrate this speculation, we analyzed GBP receptors in different types of cells by a competitive receptor binding assay with ^<125>I-GBP. The results showed that High Five cells contain two types of GBP receptors : high affinity (K_d=0.26) and low affinity (K_d=13.7), while hemocytes contain a single class of GBP receptor with a medium affinity (K_d=1.26). These results demonstrated a heterogeneity of GBP receptors at least in insect culture cells and hemocyte cells. Further, we identified two different types of GBP receptor candidates in hemocytes, approximately 60 kDa and 77 kDa proteins. The former protein does not contain any distinct domain structures other than one transmembrane domain. However, the latter protein contains one transmembrane domain, and ITAM (immunoreceptor tyrosine-based activation motif) and IgG like domains in the outer cell region and inner cell region, respectively. We further found that tyrosine residues in the cytoplasmic region of this molecule are phosphorylated by GBP. We are now trying to demonstrate whether GBP specifically binds to these cell membrane proteins.

Report

(5 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (53 results)

All 2006 2005 2004 2003 2002 Other

All Journal Article (37 results) Book (1 results) Patent(Industrial Property Rights) (2 results) Publications (13 results)

  • [Journal Article] Mechanisms of black and white stripe pattern formation in the cuticles of insect larvae.2006

    • Author(s)
      Ninomiya, Y., Tanaka, K., Hayakawa, Y.
    • Journal Title

      J.Insect Physiol. (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Regulation of growth-blocking peptide expression during embryogenesis of the cabbage armyworm.2005

    • Author(s)
      Tsuzuki, S.
    • Journal Title

      Biochem. Biophys. Res. Commun. 335

      Pages: 1078-1084

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Peptide mimics of epidermal growth factor (EGF) with antagonistic activty.2005

    • Author(s)
      Nakamura, T.
    • Journal Title

      J. Biotechnol. 116

      Pages: 211-219

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A cytokine secreted from the suboesophageal body is essential for morphogenesis of the insect head.2005

    • Author(s)
      Tsuzuki, S.
    • Journal Title

      Mech. Dev. 122

      Pages: 189-197

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mechanisms of black and white strip pattern formation in the cuticles of insect larvae.2005

    • Author(s)
      Ninomiya, Y.
    • Journal Title

      J. Insect Physiol. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regulation of growth-blocking peptide expression during embryogenesis of the cabbage armyworm.2005

    • Author(s)
      Tsuzuki, S.
    • Journal Title

      Biochem.Biophys.Res.Commun. 335

      Pages: 1078-1084

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Peptide mimics of epidermal growth factor (EGF) with antagonistic activty.2005

    • Author(s)
      Nakamura, T.
    • Journal Title

      J.Biotechnol. 116

      Pages: 211-219

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A cytokine secreted from the suboesophageal body is essential for morphogenesis of the insect head.2005

    • Author(s)
      Tsuzuki, S.
    • Journal Title

      Mech.Dev. 122

      Pages: 189-197

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mechanisms of black and white strip pattern formation in the cuticles of insect larvae.2005

    • Author(s)
      Ninomiya, Y.
    • Journal Title

      J.Insect Physiol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regulation of growth-blocking peptide expression during embryogenesis of the cabbage armyworm.2005

    • Author(s)
      Tsuzuki, S., Sekiguchi, S., Hayakawa, Y.
    • Journal Title

      Biochem.Biophys.Res.Commun. 335

      Pages: 1078-1084

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Peptide mimics of epidermal growth factor (EGF) with antagonistic activity.2005

    • Author(s)
      Nakamura, T., Takasugi, H., Aizawa, T., Yoshida, M., Mizuguchi, M., Mori, Y, Shinoda H., Hayakawa, Y., Kawano, K.
    • Journal Title

      J.Biotechnol. 116

      Pages: 211-219

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A cytokine secreted from the suboesophageal body is essential for morphogenesis of the insect head.2005

    • Author(s)
      Tsuzuki, S., Sekiguchi, S, Kamimura, M., Kiuchi, M., Hayakawa, Y.
    • Journal Title

      Mech.Dev. 122

      Pages: 189-197

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A cytokine secreted from the suboesophageal body is essential for morphogenesis of the insect head2005

    • Author(s)
      Tsuzuki, S., Sekiguchi, S, Kamimura, M., Kiuchi, M., Hayakawa, Y.
    • Journal Title

      Mech.Dev. 122

      Pages: 189-197

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The Gly-Gly linker region of the insect cytokine growth blocking peptide (GBP) is essential for activity.2004

    • Author(s)
      Yoshida, M.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 51331-51337

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Gly-Gly linker region of the insect cytokine growth blocking peptide (GBP) is essential for activity.2004

    • Author(s)
      Yoshida, M.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 51331-51337

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Gly-Gly Linker Region of the Insect Cytokine Growth Blocking Peptide (GBP) is Essential for Activity2004

    • Author(s)
      Yoshida, M., Aizawa, T., Nakamura, T., Shitara, K., Hayakawa, Y.他9
    • Journal Title

      J.Biol.Chem. 279

      Pages: 51331-51337

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 生理活性ペプチドの解析2004

    • Author(s)
      早川洋一
    • Journal Title

      植物防疫 58

      Pages: 523-528

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cause of mortality in insects under severe stress.2003

    • Author(s)
      Matsumoto, H.
    • Journal Title

      Eur. J. Biochem. 270

      Pages: 3469-3470

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Enhanced expression of genes in the larval brains of Mamestra brassicae under short day length or by feeding or L-Dopa.2003

    • Author(s)
      Uryu, M.
    • Journal Title

      Eur. J. Entomol., 100

      Pages: 245-250

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of polydnavirus genes in insect hemocyte and Sf9 culture cells.2003

    • Author(s)
      Tanaka, K.
    • Journal Title

      J. Insect Physiol. 49

      Pages: 433-440

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation and characterization of a dopa decarboxylase cDNA and the induction of its expression by an insect cytokine, growth-blocking peptide in Pseuadaletia separata.2003

    • Author(s)
      Noguchi, H..
    • Journal Title

      Insect Biochem. Molec. Biol. 33

      Pages: 209-217

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Role of aromatic residues in the structure and biological activity of the small cytokine, growth-blocking peptide (GBP).2003

    • Author(s)
      Tada, M.
    • Journal Title

      J. Biol. Chem., 278

      Pages: 10778-10783

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Insect cytokine, growth-blocking peptide, triggers off a termination system of cellular immunity by inducing its binding protein.2003

    • Author(s)
      Matsumoto, Y.
    • Journal Title

      J. Biol. Chem. 278

      Pages: 38579-38585

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cause of mortality in insects under severe stress.2003

    • Author(s)
      Matsumoto, H.
    • Journal Title

      Eur.J.Biochem. 270

      Pages: 3469-3470

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Enhanced expression of genes in the larval brains of Mamestra brassicae under short day length or by feeding or L-Dopa.2003

    • Author(s)
      Uryu, M.
    • Journal Title

      Eur.J.Entomol. 3

      Pages: 245-250

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of polydnavirus genes in insect hemocyte and Sf9 culture cells.2003

    • Author(s)
      Tanaka, K.
    • Journal Title

      J.Insect Physiol. 49

      Pages: 433-440

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation and characterization of a dopa decarboxylase cDNA and the induction of its expression by an insect cytokine, growth-blocking peptide in Pseuadaletia separata.2003

    • Author(s)
      Noguchi, H.
    • Journal Title

      Insect Biochem.Molec.Biol. 33

      Pages: 209-217

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Role of aromatic residues in the structure and biological activity of the small cytokine, growth-blocking peptide (GBP)2003

    • Author(s)
      Tada, M.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 10778-10783

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Insect cytokine, growth-blocking peptide, triggers off a termination system of cellular immunity by inducing its binding protein.2003

    • Author(s)
      Matsumoto, Y.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 38579-38585

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Analysis in the course of polydnavirus replication in ovarian cells of parasitoid wasp.2002

    • Author(s)
      Tanaka, K.
    • Journal Title

      Applied Entomol. Zool. 37

      Pages: 323-328

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detailed characterization of polydnavirus immunoevasive prot in an endoparasitoid.2002

    • Author(s)
      Tanaka, K.
    • Journal Title

      Eur. J. Biochem. 269

      Pages: 2557-2566

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression and purification of a small cytokine growth-blocking peptide from armyworm Pseudaletia separata by an optimised fermentation method using the methylotrophic yeas Pichia pastori. s.2002

    • Author(s)
      Koganesawa, N.
    • Journal Title

      Protein Expression and Purification 25

      Pages: 416-425

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Solution structure of paralytic peptide of silkworm, Bomby mori.2002

    • Author(s)
      Miura, K.
    • Journal Title

      Peptides 23

      Pages: 2111-2116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Analysis in the course of polydnavirus replication in ovarian calyx cells of parasitoid wasp.2002

    • Author(s)
      Tanaka, K.
    • Journal Title

      Applied Entomol.Zool. 37

      Pages: 323-328

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detailed characterization of polydnavirus immunoevasive proteins in an endoparasitoid.2002

    • Author(s)
      Tanaka, K.
    • Journal Title

      Eur.J.Biochem. 269

      Pages: 2557-2566

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression and purification of a small cytokine growth-blocking peptide from armyworm Pseudaletia separata by an optimised fermentation method using the methylotrophic yeast Pichia pastori.s.2002

    • Author(s)
      Koganesawa
    • Journal Title

      Protein Expression and Purification 25

      Pages: 416-425

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Solution structure of paralytic peptide of silkworm, Bombyx mori.2002

    • Author(s)
      Miura, K.
    • Journal Title

      Peptides 23

      Pages: 2111-2116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 生理活性ペプチドの解析2004

    • Author(s)
      早川 洋一
    • Total Pages
      6
    • Publisher
      日本植物防疫
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 毛乳頭細胞を活性化するためのサイトカイン2004

    • Inventor(s)
      早川洋一, 相沢智康, 河野敬一, 多田雅人
    • Industrial Property Rights Holder
      北海道大学学長
    • Industrial Property Number
      2004-226985
    • Filing Date
      2004-08-03
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 昆虫から単離した新規生理活性ペプチド2003

    • Inventor(s)
      早川洋一
    • Industrial Property Rights Holder
      北海道大学学長
    • Industrial Property Number
      2003-146551
    • Filing Date
      2003-05-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] H.Noguchi: "Isolation and characterization of a dopa decarboxylase cDNA and the induction of its expression by an insect cytokine, growth-blocking peptide in Pseuadaletia separata"Insect Biochem.Mol.Biol.. 33. 209-217 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Tanaka: "Expression of polydnavirus genes in insect hemocyte and Sf9 culture cells"J.Insect.Physiol.. 49. 433-440 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Matsumoto: "The cause of mortality in insects under severe stress"Eur.J.Biochem.. 270. 3469-3470 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Uryu: "Enhanced expression of genes in the larval brains of Mamestra brassicae under short day length or by feeding or L-Dopa"Eur.J.Entomol.. 33. 245-250 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Tada: "Role of aromatic residues in the structure and biological activity of the small cytokine, growth-blocking peptide (GBP)"J.Biol.Chem.. 278. 10778-10783 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Matsumoto: "Insect cytokine, growth-blocking peptide, triggers off a termination system of cellular immunity by inducing its binding protein"J.Biol.Chem.. 278. 38579-38585 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 早川洋一(分担執筆): "昆虫学大辞典"朝倉書店. 1220 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Tanaka: "Analysis in the course of polydnavirus replication in ovarian calyx cells of parasitoid wasp"Appl. Entomol. Zool. 37. 323-328 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Tanaka: "Detailed characterization of polydnavirus immunoevasive proteins in an endoparasitoid wasp"Eur. J. Biochem.. 269. 2557-2566 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] N.Koganesawa: "Expression and purification of a small cytokine growth-blocking peptide from armyworm Pseudaletia separata by an optimised fermentation method using the methylotrophic yeast"Protein Expression and Purification. 25. 416-425 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Aizawa: "Structure and activity of insect cytokine GBP which stimulates the EGF receptor"Mol. Cells. 14. 1-8 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Noguchi: "Isolation and characterization of a dopa decarboxylase cDNA and the induction of its expression by an insect cytokine, growth-blocking peptide in Pseuadaletia separata"Insect Biochem. Molec. Biol.. 33. 209-217 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Miura: "Solution structure of paralytic peptide of silkworm, Bombyx mori"Peptides. (in press).

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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