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Molecular Physiology of the Highly-Selective Bioaccumulation of Metal Ions

Research Project

Project/Area Number 14340264
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

MICHIBATA Hitoshi  Hiroshima University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (00111740)

Co-Investigator(Kenkyū-buntansha) HIROTA Hiroshi  RIKEN Yokohama Center, Sub Project Director (researcher), タンパク質構造機能研究グループ, プロジェクト副ディレクター(研究職) (00126153)
KANAMORI Kan  Toyama University, Faculty of Science, Professor, 理学研究科, 教授 (00019001)
UEKI Tatsuya  Hiroshima University, Graduate School of Science, Associate Professor, 大学院・理学研究科, 助教授 (10274705)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2003: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 2002: ¥9,400,000 (Direct Cost: ¥9,400,000)
Keywordsascidians / vanadium / accumulation mechanism / coordination chemistry / structural biology / ESR / NMR / EST / 金属結合タンパク質 / 立体構造 / 解離定数
Research Abstract

Ascidians accumulate extremely high concentration of vanadium ions selectively. Its concentration in the accumulating cells reaches 350 mM, which corresponds to 10 million times higher than that in sea water. The vanadium ions are stored in the vacuole of the vanadocytes at +3 oxidation state, which is rarely found in living cells.
The pH of the vacuole is very low, and the vacuole contains high concentration of sulfate ions. This unusual phenomenon can be a model system for the study of selective accumulation of metal ions.
(1) The metal binding ability, namely maximum number of metal ions that bind to each protein and the dissociation constants, of Vanabin 1 and Vanabin 2 of Ascidia sydneiensis samea were revealed. By electron spin resonance (ESR), it was found that amine nitrogens and oxygen atoms of Vanabin2 coordinate to vanadyl ions, and sulfur atoms do not.
(2) From cDNA libraries of Ciona intestinalis, five genes homologous to Vanabins were identified. Their gene structure, expression profile, and metal binding activity was examined.
(3) Expression sequence tags (EST) analysis and immobilized vanadium affinity chromatography revealed three novel vanabins, Vanabin3, Vanabin4 and VanabinP. Their expression pattern and metal binding ability was examined.
(4) By nuclear magnetic resonance (NMR) analysis, 3D structure of Vanabin2 was almost determined.
(5) Membrane transporters and channels belonging to Nramp family, metal ATPase family, chloride channel family and sulfate transporter family was cloned from vanadocytes of Ascidia sydneiensis samea. Exogenous expression of each gene in mammalian or yeast cells was performed.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] 道端 齊: "脊索動物・ホヤのバナジウム濃縮"化学と生物. 40. 687-692 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michibata, H.: "Vanadocyte is a cell holding the key to resolve the highly selective accumulation and reduction of vanadium in ascidians,"Microscop.Res.Technol.. 56. 421-434 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaguchi, N.: "Expressed sequence tag analysis of blood cells in the vanadium-rich ascidian, Ascidia sydneiensis samea - a survey of genes for metal accumulation"Zool.Sci.. 19. 1001-1008 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueki, T.: "Vanadium-binding proteins (vanabins) from a vanadium-rich ascidian Ascidia sydneiensis samea"Biochim.Biophys.Acta. 1626. 43-50 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukui, K.: "Vanadium-binding protein in a vanadium-rich ascidian Ascidia sydneiensis samea : CW and pulsed EPR studies."J.Am Chem.Soc.. 125. 6352-6353 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueki, T.: "Chloride Channel in Vanadocytes of a Vanadium-Rich Ascidian Ascidia sydneiensis samea"Comp.Biochem.Phys.B. 136. 91-98 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 植木 龍也: "ホヤ -稀少金腐バナジウムを濃縮する動物-"遺伝. 57. 10-13 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueki, T.: "Bioaccumulation of copper ions by Escherichia coil expressing vanabin genes from the vanadium-rich ascidian, Ascidia sydneiensis samea."Appl.Environ.Microbiol.. 69. 6442-6446 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Trivedi, S.: "Novel vanadium-binding proteins (Vanabins) identified in cDNA libraries and the genome of the ascidian Ciona in testinalis."Biochim.Biophys.Acta. 1630. 64-70 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaguchi, N.: "Expressed sequence tag analysis of vanadocytes in a vanadium rich ascidian, Ascidia sydneiensis samea."Mar.Biotechnol.. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michibata, H.: "Chordates -vanadium accumulation in ascidians."Kagaku To Seibutsu. 40. 687-692 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michibata, H.: "Vanadocytes, cells hold the key to resolving the highly selective accumulation and reduction of vanadium in ascidians."Microscop.Res.Tech.. 56. 421-434 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaguchi, N.: "Expressed sequence tag analysis of blood cells in the vanadium-rich ascidian, Ascidia sydneiensis samea -a survey of genes for metal accumulation."Zool.Sci.. 19. 1001-1008 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueki, T.: "Vanadium-binding proteins (vanabins) from a vanadium-rich ascidian Ascidia sydneiensis samea."Biochim.Biophys.Acta. 1626. 43-50 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukui, K.: "Vanadium-binding protein in a vanadium-rich ascidian Ascidia sydneiensis samea : CW and pulsed EPR studies."J.Am.Chem.Soc.. 125. 6352-6353 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueki, T.: "Chloride channel in vanadocytes of a vanadium-rich ascidian Ascidia sydneiensis samea."Comp.Biochem.Phys.B. 136. 91-98 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueki, T.: "Ascidians -An animal that accumulate a rare metal, vanadium."Iden. 57. 10-13 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaguchi, N.: "Expressed sequence tag analysis of vanadocytes in a vanadium-rich ascidian, Ascidia sydneiensis samea."Mar.Biotechnol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueki, T.: "Bioaccumulation of copper ions by Escherichia coli expressing vanabin genes from the vanadium-rich ascidian, Ascidia sydneiensis samea."Appl.Environ.Microbiol.. 69. 6442-6446 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Trivedi, S.: "Novel vanadium-binding proteins (vanabins) identified in cDNA libraries and the genome of the ascidian Ciona intestinalis."Biochim.Biophys.Acta. 1630. 64-70 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukui, K.: "Vanadium-binding protein a vanadium-rich ascidian Ascidia sydneiensis samea : CW and pulsed EPR studies"J.Am Chem.Soc.. 125. 6352-6353 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueki, T.: "Chloride Channel in Vanadocytes of a Vanadium-Rich Ascidian Ascidia sydneiensis samea"Comp.Biochem.Phys.B. 136. 91-98 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 植木, 龍也: "ホヤ -稀少金属バナジウムを濃縮する動物-"遺伝. 57. 10-13 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueki, T.: "Bioaccumulation of copper ions by Escherichia coli expressing vanabin genes from the vanadium-rich ascidian, Ascidia sydneiensis samea"Appl.Environ.Microbiol.. 69. 6442-6446 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Trivedi, S.: "Novel vanadium-binding proteins (Vanabins) identified in cDNA libraries and the genome of the ascidian Ciona intestinalis"Biochim.Biophys.Acta. 1630. 64-70 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamaguchi, N.: "Expressed sequence tag analysis of vanadocytes in a vanadium-rich ascidian, Ascidia sydneiensis samea"Mar.Biotechnol.. (印刷中). (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 道端 齊: "脊索動物-ホヤのバナジウム濃縮"化学と生物. 40. 687-692 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Michibata, H.: "Vanadocyte is a cell holding the key to resolve the highly selective accumulation and reduction of vanadium in ascidians"Microscop. Res. Technol.. 56. 421-434 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamaguchi, N.: "Expressed sequence tag analysis of blood cells in the vanadium-rich ascidian, Ascidia sydneiensis samea-a survey of genes for metal accumulation"Zool. Sci.. 19. 1001-1008 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ueki, T.: "Vanadium-binding proteins(vanabins) from a vanadium-rich ascidian Ascidia sydneiensis samea"Biochim. Biophys. Acta. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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