• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Comparison of chloroplast, mitochondrial and multiple nuclear DNA trees in genus Pinus subgenus Stnobus

Research Project

Project/Area Number 14340265
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 系統・分類
Research InstitutionChiba University

Principal Investigator

WATANO Yasuyuki  Chiba University, Department of Biology, Faculty of Science, Professor, 理学部, 教授 (70192820)

Co-Investigator(Kenkyū-buntansha) OHSAWA Takeshi A.  Chiba University, Department of Biology, Faculty of Science, Research Associate, 理学部, 助手 (50213682)
MINAKA Nobuhiro  National Institute for Agro-Environmental Sciences, Senior scientist, 地球環境部, 主任研究員
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥10,100,000 (Direct Cost: ¥10,100,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsPinus / introgessive hybridization / cytoplasmic capture / Molecular phylogeny / mitochondrial DNA / chloroplast DNA / nuclear DNA / cytoPlasmic capture / mitochondrial DNA
Research Abstract

Hybridization and introgression have been considered to be one of the main factors that lead to phylogenetic incongruence among different data sets. In the plants of Pinaceae, chloroplast and mitochondrial DNAs may have different evolutionary histories through introgression because cpDNA and mtDNA are inherited paternally and maternally, respectively. In fact, Pinus species have insufficient reproductive barriers, and many examples of introgressive hybridization have been reported. Pinus species, therefore, could be susceptible to phylogenetic bias by hybridization and introgression. In the present study, we reconstructed cpDNA tree, mtDNA tree, and four nuclear DNA trees in the genus Pinus subgenus Strobus, and compared them in order to detect cryptic and ancient introgression episodes which caused incongruence among gene trees. Four nrDNA trees were constructed based on partial sequences of GapCp, PgiC and ESTs of Pinus taeda (PtIFG_9008, PtIFG_0464). MtDNA tree was generated based on partial sequences of intron2 of nad1 and intronl of nad5 genes. CpDNA tree was based on the dataset of Wang et al. (1999) and our additional data. In nrDNA trees, species of the section Strobus were monophyletic, and the species of the other section (Parrya) were paraphyletic. In the sec. Parrya, two species of the subsection Gerardianae formed a clade, which was a sister to the sec. Strobus. CpDNA tree was consistent with the nrDNA trees in the relationship among sections and subsections. On the other hand, mtDNA tree showed three clades, and in the two of them, each clade consisted of the combined members both from sec. Strobus and from sec Parrya subsec. Gerardianae. Because phylogenetic relationships suggested by the nrDNA and cpDNA trees are congruent and also reasonable in morphological respects, the cause of incongruence could be attributed to the bias in the mtDNA tree. Possibly, mtDNA introgression had occurred anciently between species of different sections.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (5 results)

All 2004 Other

All Journal Article (4 results) Publications (1 results)

  • [Journal Article] Genetic structure of hybrid zones between Pinus pumila and P.parviflora var.pentaphylla(Pinaceae) revealed by molecular hybrid index analysis2004

    • Author(s)
      Watano, Y., Kanai, A., Tani, N.
    • Journal Title

      American Journal of Botany 91

      Pages: 65-72

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ハイマツとキタゴヨウ,交雑する樹木のはなし2004

    • Author(s)
      綿野 泰行
    • Journal Title

      プランタ 96

      Pages: 49-55

    • NAID

      40006542394

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Genetic structure of hybrid zones between Pinus pumila and P.parviflora var.pentaphylla (Pinaceae) revealed by molecular hybrid index analysis2004

    • Author(s)
      Watano, Y., Kanai, A., Tani, N.
    • Journal Title

      American Journal of Botany 91

      Pages: 65-72

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Introgressive hybridization between Pinus pumila and P.parviflora var.pentaphylla2004

    • Author(s)
      Watano, Y.
    • Journal Title

      PULANTA(Japanese Journal) 96

      Pages: 49-55

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Watano, Y., Kanai, A., Tani, N.: "Genetic structure of hybrid zones between Pinus pumila and P. parviflora var. pentaphylla (Pinaceae) revealed by molecular hybrid index analysis"American Journal of Botany. 91. 65-72 (2004)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi