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Analyses of speciation mechanism in a species of hybrid origin, using flowering genes as indicator

Research Project

Project/Area Number 14340266
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 系統・分類
Research InstitutionKanazawa University

Principal Investigator

KOFUJI Rumiko  Kanazawa University, Graduate School of Natural Science and Technology, Research Associate, 自然科学研究科, 助手 (40324066)

Co-Investigator(Kenkyū-buntansha) UEDA Kunihiko  Kanazawa University, Graduate School of Natural Science and Technology, Professor, 自然科学研究科, 教授 (60184925)
KINOSHITA Eiichiro  Kanazawa University, Institute of Nature and Environmental Technology, Associate Professor, 自然計測応用研究センター, 助教授 (70234317)
ISHIDA Kenichiro  Kanazawa University, Graduate School of Natural Science and Technology, Associate Professor, 自然科学研究科, 助教授 (30282198)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥9,100,000 (Direct Cost: ¥9,100,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2002: ¥5,100,000 (Direct Cost: ¥5,100,000)
KeywordsDrosera rotundifolia / Drosera spatulata / Drosera tokaiensis / life history / speciation / hybrid origin / allopolyploid / genetic analysis / 遺伝子発現 / 複二倍体 / 交雑起源種 / 花期
Research Abstract

This project aimed to clarify what kind of reorganization in the genome and gene expression was occurred in the speciation process of an amphidiplloid-species of hybrid origin, Drosera tokaiensis, of which parents are thought to be D.retundifolia and D.spatulata.
D.tokaiensis is distributed in Kinki, Tokai, and Hokunku districts of Japan. We chose to study on four populations, Kosugi (northern limit), Nata, Ryuo, and Taketoyo (southern limit). Life history phenotypes were analyzed in field and in a greenhouse. For genetic analyses, individuals cultured in an incubator were used. Results obtained are the following.
1.Life history trait : Flowering time of D.tokaiensis was intermediate of parental species and similar in all populations. The leaf number of D.tokaiensls at the flowering time varied, compare to those of parental species. The average leaf number was the largest in D.spatulata which flowers the earliest, intermediate in D.tokaiensis, and the smallest in D.rotundifolia which flowers last. This is the opposite phenotype of what is expected from the act of LFY, the most studied flowering gene. The seed germination phenotype was differed between populations, but similar within each population expect Taketoyo.
Genetic analyses : Both chloroplast and nuclear DNAs sequenced from the four populations of D.tokaiensis were identical. The chloroplast DNA sequences were same as those of D.spatulata. For nuclear DNA, ITS1-5.8S rDNA-ITS2 region and atpG region, there were three different sequences in D.tokaiensis, two of the three were identical to two sequences obtained from D.spatulata and the rest was identical to one sequence obtained from D.rotundifolia. These suggest that D.tokaiensis recently originated from D.spatulata as mother and D.rotundifolia as father. An RT-PCR experiment showed that the different copy of the gene might expressed in different individuals of D.tokaiensis.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report

Research Products

(34 results)

All 2005 2004 2003 2002 Other

All Journal Article (28 results) Publications (6 results)

  • [Journal Article] A new variety, Scabiosa japonica var.breviligula (Dipsacaceae) from Tokai District, Central Japan.2005

    • Author(s)
      Suyama C, Ueda K.
    • Journal Title

      The Journal of Japanese Botany 80

      Pages: 14-21

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phylogeny of the psbC gene, coding a photosystem II component CP43, suggests separate origins for the peridinin- and the fucoxanthin derivative-containing plastids of dinoflagellates.2005

    • Author(s)
      Takishita, K. et al.
    • Journal Title

      Phycologia 44

      Pages: 26-34

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Life history traits of an amphidiploid, Drosera tokaiensis, and the parental species, D.rotundifolia and D.spatulata (Droseraceae).2004

    • Author(s)
      Nakano, M. et al.
    • Journal Title

      Plant Species Biology 19

      Pages: 59-72

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chloroplast Phylogeny Indicates that Bryophytes are Monophyletic.2004

    • Author(s)
      Nishiyama, T. et al.
    • Journal Title

      Molecular Biology and Evolution 21

      Pages: 1813-1819

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Life history traits and coexistence of an amphidiploid, Drosera tokaiensis, and its parental species, D.rotundifolia and D.spatulata (Droseraceae).2004

    • Author(s)
      Nakano M, Kinoshita E, Ueda K.
    • Journal Title

      Plant Species Biology 19

      Pages: 59-72

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chloroplast Phylogeny Indicates that Bryophytes are Monophyletic.2004

    • Author(s)
      Nishiyama T, Wolf PG, Kugita M, Sinclair RB, Sugita M, Sugiura C, Wakasugi T, Yamada K, Yoshinaga K, Yamaguchi K, Ueda K, Hasebe M.
    • Journal Title

      Molecular Biology and Evolution 21

      Pages: 1813-1819

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phylogeny of Nuclear-Encoded Plastid-Targeted GAPDH Gene Supports Separate Origins for the Peridinin-and the Fucoxanthin Derivative-Containing Plastids of Dinoflagellates.2004

    • Author(s)
      Takishita K, Ishida K, Maruyama T.
    • Journal Title

      Protist 155

      Pages: 447-458

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Carotenoid compositions of Cladophora balls (Aegagropila linnaei) and some members of the Cladophorales (Ulvophyceae, Chlorophyta) : their taxonomic and evolutionary implication.2004

    • Author(s)
      Yoshii Y, Hanyuda T, Wakana I, Miyaji K, Arai S, Ueda K, Inouye I.
    • Journal Title

      Journal of Phycology 40

      Pages: 1170-1177

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phylogeny and taxonomy of freshwater Bangia (Bangiales, Rhodophyta) in Japan.2004

    • Author(s)
      Hanyuda T, Suzawa Y, Arai S, Ueda K, Kumano S.
    • Journal Title

      The Journal of Japanese Botany 79

      Pages: 262-268

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Morphology, ontogeny and phylogeny of freshwater green alga, Blindingia minima var.ramifera (Ulvales, Ulvo-phyceae) from Nikko, Tochigi Prefecture, Japan.2004

    • Author(s)
      Iima M, Hanyuda T, Ueda K, Yoshizaki M, Ebata H.
    • Journal Title

      Japanese Journal of Phycology 52 (Suppl.)

      Pages: 177-182

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Life history traits of an amphidiploid, Drosera tokaiensis, and the parental species, D.rotundifolia and D.spatulata(Droseraceae).2004

    • Author(s)
      Nakano, M. et al.
    • Journal Title

      Plant Species Biology 19

      Pages: 59-72

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Phylogeny and taxonomy of freshwater Bangia(Bangiales, Rhodophyta) in Janan.2004

    • Author(s)
      Hayuda, T. et al.
    • Journal Title

      Japanese Journal of Botany 79

      Pages: 1813-1819

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Morphology, ontogeny and phylogeny of freshwater green alga, Blindingia minima var.ramifera(Ulvales, UIvo-phyceae) from Nikko, Tochigi Prefecture, Japan.2004

    • Author(s)
      Iima, M. et al.
    • Journal Title

      Jpn.J.Phycol. 52(Suppl.)

      Pages: 177-182

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Phylogeny of nuclear-encoded plastid-targeted GAPDH gene supports separate origins for fucoxanthin- and derivative-containing olastids of dinoflagellate.2004

    • Author(s)
      Takishita, K. et al.
    • Journal Title

      Protist 155

      Pages: 447-458

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Development of polymorphic microsatellite markers in Arisaema serratum (Thunb.) Schott, Araceae.2003

    • Author(s)
      Nishizawa, T. et al.
    • Journal Title

      Moleculor Ecology Notes 3

      Pages: 32-34

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evolution and divergence of the MADS-box gene family based on genome -wide expression analyses.2003

    • Author(s)
      Kofuji, R. et al.
    • Journal Title

      Molecular Biology and Evolution 20

      Pages: 1963-1977

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of auxin and a homeodomain-leucine zipper I gene in rhizoid development of the moss Physcomitrella patens.2003

    • Author(s)
      Sakakibara, K. et al.
    • Journal Title

      Development 130

      Pages: 4835-4846

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of polymorphic microsatellite markers in Arisaema serratum (Thunb.) Schott, Araceae.2003

    • Author(s)
      Nishizama T, Kawahara T, Kinoshita E, Ueda K, Watano Y.
    • Journal Title

      Molecular Ecology Notes 3

      Pages: 32-34

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evolution and divergence of MADS-box gene family based on genome-wide expression analyses.2003

    • Author(s)
      Kofuji R, Sumikawa N, Yamasaki M, Kondo K, Ueda K, Ito M, Hasebe M.
    • Journal Title

      Molecular Biology and Evolution 20

      Pages: 1963-1977

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of auxin and a homeodomain-leucine zipper I gene in rhizoid development of the moss Physcomitrella patens.2003

    • Author(s)
      Sakakibara, K., Nishiyama, T., Sumikawa, N., Kofuji, R., Murata, T., Hasebe, M.
    • Journal Title

      Development. 130

      Pages: 4835-4846

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ultrastructural changes in Neurospora cells undergoing senescence induced by kalilo plasmids.2003

    • Author(s)
      Bok J-W, Ishida K, Griffiths AJF
    • Journal Title

      Mycologia 95

      Pages: 500-505

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An enigmatic GAPDH gene in the symbiotic dinoflagellate genus Symbiodinium and its related species (the order Suessiales) : Possible lateral gene transfer between two eukaryotic algae, dinoflagellate and euglenophyte.2003

    • Author(s)
      Takishita K, Ishida K, Maruyama T.
    • Journal Title

      Protist 154

      Pages: 443-454

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Lateral gene transfer and the evolution of plastid-targeted proteins in the secondary plastid-containing alga, Bigelowiella natans.2003

    • Author(s)
      Archibald JM, Rogers MB, Toop M, Ishida K, Keeling PJ.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 100

      Pages: 7678-7683

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A thylakoidal processing peptidase from the heterokont alga Heterosigma akashiwo.2003

    • Author(s)
      Chaal BK, Ishida K, Green BR.
    • Journal Title

      Plant Molecular Biology 52

      Pages: 463-472

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chloroplast DNA phylogeography of Fagus crenata (Fagaceae) in Japan.2002

    • Author(s)
      Fujii, N. et al.
    • Journal Title

      Plant Syst.Evol. 232

      Pages: 21-33

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Second-hand and third-hand chloroplasts in dinoflagellates : phylogeny of oxygen-evolving enhancer 1 (PsbO) protein reveals replacement of a nuclear-encoded plastid gene by that of a haptophyte tertiary endosymbiont.2002

    • Author(s)
      Ishida K, Green BR.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 99

      Pages: 9294-9299

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phylogenetic relationships within Cladophorales (Ulvophyceae, Chlorophyta) inferred from 185 rRNA gene sequences, with special reference to Aegagropila linnaei.2002

    • Author(s)
      Hanyuda T, Wakana I, Arai S, Miyaji K, Watano Y, Ueda K.
    • Journal Title

      Journal of Phycology 38

      Pages: 564-571

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chloroplast DNA phylogeography of Fagus crenata (Fagaceae) in Japan.2002

    • Author(s)
      Fujii N, Tomaru N, Okuyama K, Koike T, Mikami T, Ueda K.
    • Journal Title

      Plant Systematics and Evolution 232

      Pages: 21-33

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Nakano, M. et al.: "Life history traits of an amphidiploid, Drosera tokaiensis, and the parental species, D.rotundifolia and D.spatulata(Droseraceae)"Plant Species Biology. 19(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nishizawa, T. et al.: "Development of polymorphic microsatellite markers in Arisaema serratum(Thunb.) Schott.Araceae"Moleculor Ecology Notes. 3. 32-34 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kofuji, R. et al.: "Evolution and divergence of the MADS-box gene family based on genome-wide expression analyses"Molecular Biology and Evolution. 20・12. 1963-1977 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakakibara, K. et al.: "Involvement of auxin and a homeodomain-leucine zipper I gene in rhizoid development of the moss Physcomitrella patens"Development. 130・3. 4835-4846 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Chaal, B.K.et al.: "A thylakoidal processing peptidase from the heterokont alga Heterosigma akashiwo"Plant Molecular Biology. 52・2. 463-472 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Archibald, J.M et al.: "Lateral gene transfer and the evolution of plastid-targeted proteins in the secondary plastid-containing alga, Bigelowiella natans"Proceedings of the National Academy of Sciences. 100・13. 7678-7683 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2002-03-31   Modified: 2016-04-21  

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