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Microbial community analysis of enhanced biological phosphorus removal process and identification of polyphosphate accumulating organisms by molecular approach

Research Project

Project/Area Number 14350283
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionThe University of Tokyo

Principal Investigator

MINO Takashi  The University of Tokyo, Graduate school of Frontier Sciences, Professor, 大学院・新領域創成科学研究科, 教授 (60166098)

Co-Investigator(Kenkyū-buntansha) KURISU Futoshi  The University of Tokyo, Graduate school of Engineering, Lecture, 大学院・工学系研究科, 講師 (30312979)
FURUMAI Hiroaki  The University of Tokyo, Graduate school of Engineering, Professor, 大学院・工学系研究科, 教授 (40173546)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2003: ¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 2002: ¥8,400,000 (Direct Cost: ¥8,400,000)
KeywordsActivated sludge / anaerobic-aerobic / enhanced biologicall phosphorus removal / 16S-rDNA / DGGE / FISH / polyphosphate accumulating organisms / Rhodocyclus related PAOs
Research Abstract

Fundamental research about enhanced biological phosphorus removal (EBPR) process is now making great progress because of the new microbial community analysis methods such as molecular techniques. This research was carried to reveal the relationship between microbial community and function of EBPR sludge using 16S-rDNA approach.
In this research, at first, laboratory scale reactors were operated using acetate, glutamate, propionate, glucose or peptone as a main carbon source respectively. The expression of phosphorus removal activity was monitored by batch experiments and the microbial community change was monitored by Denaturing gradient gel electrophoresis (DGGE) using 16S-rDNA fragments. By comparing phosphorus removal activity and microbial community change, polyphosphate accumulating organisms were screened. In addition, by sequencing the whole length of 16S-rDNA of target bacteria by using cloning method and developing gene probes which can specifically detect the target bacteria and combining with the polyphosphate staining method and fluorescent in situ hybridization, capability of accumulating polyphosphate by screened bacteria was investigated.
As a result, it was demonstratedd that gram-positive bacteria with high GC content accumulated polyphosphate in the reactor fed with mainly peptone and this gram positive bacteria was considered as the second putative polyphosphate accumulating organisms (PAOs) after Rhodocyclus related bacteria, which has been already considered as PAOs candidates. In addition, as another bacteria which accumulated polyphosphate but was not Rhodocyclus related bacteria nor gram positive bacteria was detected, it was clearly shown that still unknown PAOs should exist. In this way, it was shown that the EBPR is achieved by the combination of several PAOs

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Onuki, M., Satoh, H, Mino, T: "Analysis of microbial community that performs enhanced biological phosphorus removal in activated sludge fed with acetate"Proceedings of the 1^<st> Asian Environmental Research Alliance Partnership Symposium. 207-214 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 岡本 真由子: "アスパラギン酸を炭素源として運転した生物学的リン除去プロセスにおける微生物群集解析"第58回土木学会年次学術講演会講演概要集. 第7部. 97 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Onuki, M., Satoh, H., Mino, T.: "Analysis of microbial community that performs enhanced biological phosphorus removal in activated sludge fed with acetate"Proceeding of the 1st Asian Environmental Research Alliance Partnership Symposium (Taipei Taiwan). 207-214 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okamoto M., Fukushima T., Mino T., Onuki M.: "Microbial community analysis of enhanced biological phosphorus removal process sludge fed with aspartate"Proceeding of the 58th annual conference of the Japanese Society of Civil Engineering. 97 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary

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Published: 2002-04-01   Modified: 2016-04-21  

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