| Project/Area Number |
14350441
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
工業分析化学
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| Research Institution | Gunma University |
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Principal Investigator |
TSUNODA Kin-ichi Gunma Univerisity, Faculty of Engineering, Professeor, 工学部, 教授 (30175468)
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| Co-Investigator(Kenkyū-buntansha) |
ODAKE Tamao Gunma Univerisity, faculty of Engineering, lecturer, 工学部, 講師 (10301128)
UMEMURA Tomonari Nagoya University, Graduate School of Engineering, Associate Professeor, 大学院・工学研究科, 助教授 (10312901)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥12,400,000 (Direct Cost: ¥12,400,000)
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| Keywords | ICP-MS / ESI-MS / capillary electrophresis / micro-HPLC / monolith column / capillary electro-chromatography |
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| Research Abstract |
Various types of polymethacrylate-based monolithic capillary columns were newly prepared for the development of hyphenated instruments such as micro-HPLC-ICP-MS and CEC-ICP-MS. Strong cation-exchange monolithic capillary columns were constructed for ion chromotography by radical polymerization of glycidyl methacrylate (GMA) and ethylene dimethacrylate in a 250-μm-i.d. fused silica capillary and its subsequent sulfonatiori based on ring opening of epoxides with 1 M Na_2SO_3. The chromotographic performance of the columns was evaluated through common cations such as Na^+,NH_4^+,K^+,Mg^<2+> and Ca^<2+>. For an example, they could be well separated from one another on a 15 cm column with a capacity of 0.15 mequiv/mL where the pressure drop of this 15 cm column was ca. 1 MPa at a normal linear velocity of 1 mm/s (a flow rate of 3 μL/min) and the number of theoretical plates for the cations was above 3000 plates/15 cm. Moreover, this column could withstand high linear velocities of at least 10 mm/s. This GMA-based approach was extended to the preparation of strong anion-exchange columns where quaternary ammonium groups were introduced by the ring opening reaction of the epoxides with alkylamine compounds followed by the reaction with benzyl or alkyl halides. Reversed-phase columns were also prepared by radical polymerization of various alkyl methacrylates and ethylene glycol dimethacrylate. Moreover, CEC columns which show reversed-phase separation modes were also developed with this GMA-based approach.
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