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Molecular basis of mechanisms of dominance relationships among S haplotypes in self-incompatible Brassicaceae.

Research Project

Project/Area Number 14360002
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionIwate University

Principal Investigator

WATANABE Masao  Iwate University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (90240522)

Co-Investigator(Kenkyū-buntansha) SUZUKI Go  Osaka Kyoiku University, Faculty of Education, Associate Professor, 教育学部, 助教授 (10314444)
TAKADA Yoshinobu  JSPS, DC1 Researcher, 特別研究員
KAKIZAKI Tomohiro  JSPS, DC1 Researcher, 特別研究員
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2004: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2003: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2002: ¥6,200,000 (Direct Cost: ¥6,200,000)
KeywordsCruciferous plants / Self-incompatibility / self / non-self recognition / S multiple alleles / Dominance relationships / SP11 gene / Methylation / Regulation of gene expression
Research Abstract

Self-incompatibility (SI) systems prevent self-fertilization and promote out-crossing by rejecting pollen from plants with the same S haplotype. The Brassica SI system is controlled sporophytically by multiple alleles at the single locus, termed S locus. Because of sporophytic control of Brassica SI, dominance relationships among S haplotypes are observed in both stigma and pollen. We performed test-crosses between S heterozygotes and their respective parental S homozygotes for four of these class-II S haplotypes, and observed a linear dominance relationship on the pollen side. In order to determine how this dominance relationship is controlled, RNA gel blot analyzes were performed for six S heterozygotes and their respective parental S homozygotes by using the corresponding SP11 cDNA clone as a probe. In all six S heterozygotes, SP11 derived from a dominant S haplotype was predominantly expressed, and SP11 derived from a recessive S haplotype was repressed. Therefore, the linear dominance relationship of the SI phenotype on the pollen side is regulated by the expression of SP11 gene.
We determined the nucleotide sequences of the genomic clone of three class-II S haplotypes that contained corresponding SP11. These contain the part of the 5'-flanking region of the respective SRK. The distance of SP11 and SRK is about 10kbp in each S haplotypes. This result indicates that the relative position of SP11 and SRK is conserved. Therefore, the region between SP11 and SRK was named the "S-intergenic region". Then, we cloned class-II SRK, and compared these with other class-II SRK. The predicted amino acid sequence similarity within class-II SRK is about 90 to 95%, though the hypervariable region of the S domain is diverted. These results indicate not only similarity of S genes but that the physical position of their SP11 and SRK is conserved in the class-II S haplotypes.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (35 results)

All 2004 2003 2002 Other

All Journal Article (20 results) Book (3 results) Publications (12 results)

  • [Journal Article] Tandem duplication of SP11 gene at the S locus of S15 haplotype in Brassica oleracea.2004

    • Author(s)
      Shiba, H.
    • Journal Title

      Genes Genet.Syst. 79

      Pages: 87-93

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Genetic analysis intra-species unilateral incompatibility between cultiver Osome and Turkish line in Brassica rapa (syn.campestris) L.2004

    • Author(s)
      Takada, Y.
    • Journal Title

      Sex.Plant Reprod. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 「高等植物の生殖器官形成と受粉・受精過程の分子遺伝学」を企画して2004

    • Author(s)
      渡辺正夫
    • Journal Title

      GSJコミュニケーションズ 79

      Pages: 22-22

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Genetic analysis of infra-species unilateral incompatibility between cultiver Osome and Turkish line in Brassica tapa (syn.campestris)2004

    • Author(s)
      Takada, Y.
    • Journal Title

      Sex.Plant Reprod. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic analysis of intra-species unilateral incompatibility between cultiver Osome and Turkish line in Brassica rapa (syn.campestris) L.2004

    • Author(s)
      Takada, Y.
    • Journal Title

      Sex.Plant Reprod. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Linear dominance relatioship within class-II S haplotypes in pollen is directed by the expression of SPIT in Brassica self-incompatibility.2003

    • Author(s)
      Kakizaki, T.
    • Journal Title

      Plant Cell Physiol. 44

      Pages: 70-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genomic organization of the S1 locus of the S12 haplotypes of Brassica raga (syn.B.campestris).2003

    • Author(s)
      Shiba, H.
    • Journal Title

      Biosci.Biotech.Biochem. 67

      Pages: 622-626

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The S haplotypes lacking SLG in the genome of Brassica raga.2003

    • Author(s)
      Suzuki, G.
    • Journal Title

      Plant Cell Rep. 21

      Pages: 911-915

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A membrane-anchored protein kinase involved in Brassica self-incompatibility signalling.2003

    • Author(s)
      Murase, K
    • Journal Title

      Science 303

      Pages: 1516-1519

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] アブラナ科植物の自家不和合性の認識反応を制御する遺伝子の分子生物学的解剖2003

    • Author(s)
      渡辺正夫
    • Journal Title

      The Annals of Intelligent Cosmos Academic Foundation 8

      Pages: 13-16

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Linear dominance relatioship within class-II S haplotypes in pollen is directed by the expression of SP11 in Brassica self-incompatibility.2003

    • Author(s)
      Kakizaki, T.
    • Journal Title

      Plant Cell Physiol. 44

      Pages: 40-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genomic organization of the S locus of the S12 haplotypes of Brassica tapa (syn.B.campestris).2003

    • Author(s)
      Shiba, H.
    • Journal Title

      Biosci.Biotech.Biochem. 67

      Pages: 622-626

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The S haplotypes lacking SLG in the genome of Brassica rapa.2003

    • Author(s)
      Suzuki, G.
    • Journal Title

      Plant Cell Rep. 21

      Pages: 911-915

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A membrane-anchored protein kinase involved in Brassica self-incompatibility signaling.2003

    • Author(s)
      Murase, K.
    • Journal Title

      Science 303

      Pages: 1516-1519

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dominant/recessive relationship in pollen expression of self-incompatibility is determined by the transcriptional level of the pollen determinant gene in the S-heterozygotes of Brassica.2002

    • Author(s)
      Shiba, H.
    • Journal Title

      Plant Cell 14

      Pages: 491-504

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility fator SRK of Brassica campestris.2002

    • Author(s)
      Matsushita, Y.
    • Journal Title

      J.Exp.Bot. 53

      Pages: 765-767

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of S-alleles using PCR-CAPS of the S-locus receptor kinase (SRK) gene in breeding lines of Brassica oleracea.2002

    • Author(s)
      Park, J.-I.
    • Journal Title

      Plant Breed. 121

      Pages: 192-197

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dominant/recessive relationship in pollen expression of self-incompatibility is determined by the transcriptional level of the pollen determinant gene in the S-heterozygotes of Brassica.2002

    • Author(s)
      Shiba, H.
    • Journal Title

      Plant Cell

      Pages: 491-504

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility fater SRK of Brassica campestris.2002

    • Author(s)
      Matsushita, Y.
    • Journal Title

      J.Exp.Bot.

      Pages: 765-767

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of S-alleies using PCR-CAPS of the S-locus receptor kinase (SRK) gene in breeding lines of Brassica oleracea.2002

    • Author(s)
      Park, J.-I.
    • Journal Title

      Plant Breed. 121

      Pages: 192-197

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 植物育種学辞典2004

    • Author(s)
      渡辺正夫
    • Publisher
      培風館(in press)
    • Related Report
      2004 Annual Research Report
  • [Book] 生態学事典(巌佐庸ら編)2003

    • Author(s)
      渡辺正夫
    • Total Pages
      682
    • Publisher
      共立出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 植物栄養・肥料の事典(植物栄養・肥料の事典編集委員会編)2002

    • Author(s)
      土屋亨
    • Total Pages
      697
    • Publisher
      朝倉書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Shiba, H: "Genomic organization of the S locus region of Brassica"Biosci.Biotech.Biochem.. 67. 622-626 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki, G.: "The S haplotypes lacking SLG in the genome of Brassica rapa"Plant Cell Rep.. 21. 911-915 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Murase, K.: "A membrane-anchored protein kinase involved in Brassica self-incompatibility signalling"Science. 303. 1516-1519 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 渡辺正夫: "アブラナ科植物の自家不和合性の認識反応を制御する遺伝子の分子生物学的解剖"The Annals of Intelligent Cosmos Academic Foundation. 8. 13-16 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 渡辺正夫: "生態学事典"巌佐庸ら編(共立出版). 682 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shiba, H.: "Dominant/recessive relationship in pollen expression of self-incompatibility is determined by the transcriptional level of the pollen determinant gene in the S-heterozygotes of Brassica"Plant Cell. 14. 491-504 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsushita, Y.: "Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility fator SRK of Brassica campestris"J. Exp. Bot.. 53. 765-767 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Park, J.-I.: "Identification of S-alleles using PCR-CAPS of the S-locus receptor kinase (SRK) gene in breeding lines of Brassica oleracea"Plant Breed. 121. 192-197 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kakizaki, T.: "Linear dominance relationship within class-II S haplotypes in pollen is directed by the expression of SP11 in Brassica self-incompatibility"Plant Cell Physiol.. 44. 70-75 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shiba, H.: "Genomic organization of the S locus of the S12 haplotypes of Brassica rapa (syn. B. campestris)"Biosci. Biotech. Biochem.. 67(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki, G.: "The S haplotypes lacking SLG in the genome of Brassica rapa"Plant Cell Rep.. 21(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 土屋亨: "植物栄養・肥料の事典"植物栄養・肥料の事典編集委員会編(朝倉書店). 697 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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