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Structure and function of the genes specific for reproductive and vegetative organs i n Rosaceae (apple and rose) and molecular breeding

Research Project

Project/Area Number 14360019
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionGifu University

Principal Investigator

MATSUMOTO Shogo  Gifu Univ., Biology, Associate Prof., 教育学部, 助教授 (90241489)

Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥12,300,000 (Direct Cost: ¥12,300,000)
Keywordsapple / rose / Malus / Rosa / self-incompatibility / parthenocarpy / flower development / thorn
Research Abstract

(1)The S-locus genes (Si-and Sz-RNase) in the pistil were doped and sequenced from the apple (Malus x domestica Borkh.) ailtivar McIntosh. We developed S-allele specific polymerase chain reaction (PCR) -digestion method for identification the S-alleles. Using the method, we analyzed S-genotype of 310 apple species, cultivars and selections. As the parents of the some of the apple cultivars were unknown and others were uncertain, we detennined accurate parents of eight apple cult ivars using SSR markers.
(2)For unification of the alphabetical S-RNase allele designation in Japan to the European numerical designations, we clarified that the S_<a^->, S_<b^->, S_<c^->, S_<d^->, S_<e^->, S_<f^->, S_<g^->, S_<h^->, S_<i^->, S_<z^->-RNase alleles, respectively, to the S_<2^->, S_<3^->, S_<9^->, S_<7^->, S_<28^->, S_<1^->, S_<20^->, S_<24^->, S_<10^-> and S_<25^->-RNase allele. We resolved the confusion of the numerical S-RNase allele designations, and established the standardized S-RNase allele … More designation of apple.
(3)We isolated three cDNA clones (MdiPI, MdTM6 and MdiMADS13) homologous to class-B MADS-box protein from Malus x domesirca Borkh. 'Indo'. MdTM6 and MdiMADS13 genes showed the same expression patterns, and are close to TM6 lineage members. This is the first case of two TM6 lineage genes existing in one plant. Producing transgenic apple possessing apetalous flowers and parthenocarpic fruit is under propressing.
(4)We isolated rose MADS-box genes 'MASAKO BP, B3 and euB3' homologous to class-B floral identity genes. Functional analyses using transgenic Arabidopsis with ectopically expressed MASAKO BP and B3 or MASAKO BP and euB3 under the control of CaMV35S promoter produced flowers with sepal-to-petal and pistil-to-stamen or sepals-to-petals, suggesting that MASAKO BP and B3, and MASAKO B3 and euB3 are involved in petal and stamen identification in rose.
(5)The transgenic plants with ectopically expressed rose MADS -box genes 'MASAKO C1 and D1' homologous to class-C floral identity genes under the control of CaMV35S promoter produced flowers with sepa1-to-carpel and petal-to-stamen transformation in Arabidopsis and sepal-to-cazpel transformation in Torenia. These results suggest that both genes have similar function and are involved in stamen and carpel identity in the rose.
(6)We isolated Rose MADS-box genes 'MASAKO S1 and S3' homologous to class-E floral organ identity genes. We found that MASAKO S1 and S3 are homologues to SEPALLATA (SEP)1/2 and 3, respectively. The expression patterns and cDNA structures of MASAKO S1 and S3 in the green rose flower were the same as those in the normal flower of Rosa rugasa, suggesting that homeotic changes in the green rose flower might not develop into mutations of the MADS-box genes alone. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (34 results)

All 2005 2004 2003 2002 Other

All Journal Article (24 results) Publications (10 results)

  • [Journal Article] Parent identification of eight apple cultivars by S-RNase analysis and SSR markers2005

    • Author(s)
      K.Kitahara
    • Journal Title

      HortScience 40

      Pages: 314-317

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Parent identification of eight apple cultivars by S-RNase analysis and SSR markers.2005

    • Author(s)
      K.Kitahara, S.Matsumoto, T.Yamamoto, J.Soejima, T.Kimura, H.Komatsu, K.Abe
    • Journal Title

      HortScience 40

      Pages: 314-317

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cloning and characterization of apple class B MADS-box genes including a novel AP3 homologue MdTM6.2004

    • Author(s)
      K.Kitahara
    • Journal Title

      J.Japan.Soc.Hort.Sci. 73

      Pages: 208-215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ectopic expression of the rose AGAMOUS-like MADS-box genes ‘MASAKO C1 and D1' causes similar homeotic transformation of sepal and petal in Arabidopsis and sepal in Torenia.2004

    • Author(s)
      K.Kitahara
    • Journal Title

      Plant Science 166

      Pages: 1245-1252

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cloning and characterization of apple class B MADS-box genes including a novel AP3 homologue MdTM6.2004

    • Author(s)
      K.Kitahara, T.Ohtsubo, J.Soejima, S.Matsumoto
    • Journal Title

      J.Japan.Soc.Hort.Sci. 73

      Pages: 208-215

    • NAID

      110001803516

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ectopic expression of the rose AGAMOUS-like MADS-box genes 'MASAKO C1 and D1' causes similar homeotic transformation of sepal and petal in Arabidopsis and sepal in Torenia.2004

    • Author(s)
      K.Kitahara, Y.Hibino, R.Aida, S.Matsumoto
    • Journal Title

      Plant Science 166

      Pages: 1245-1252

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cloning and characterization of apple class B MADS-box genes including a novel AP3 homologue ‘MdTM6'2004

    • Author(s)
      K.Kitahara
    • Journal Title

      J.Japan Soc.Hort.Sci. 73

      Pages: 208-215

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Ectopic expression of the rose AGAMOUS-like MADS-box genes MASAKO C1 and D1'causes similar homeotic transformation of sepal and petal in Arabidosis and sepal in Torenia2004

    • Author(s)
      K.Kitahara
    • Journal Title

      Plant Science 166

      Pages: 1245-1252

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Sallele genotype of apple cultivars and selections.2003

    • Author(s)
      S.Matsumoto
    • Journal Title

      Acta Horticulturae 622

      Pages: 389-396

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] S-allele genotypes of apple pollenizers and lineages including those resistant to scab.2003

    • Author(s)
      S.Matsumoto
    • Journal Title

      J.Hortic.Sci.Biotechnol. 78

      Pages: 634-637

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Partial genomic sequences of S_<6^->, S_<12^->, S_<13^->, S_<14^->, S_<17^->, S_<19^->, and S_<21^->RNases of apple and their allele designations2003

    • Author(s)
      S.Matsumoto
    • Journal Title

      Plant Biotechnology 20

      Pages: 323-329

    • NAID

      10012536444

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] S-allele genotype of apple cultivars and selections.2003

    • Author(s)
      S.Matsumoto, K Kitahara, Y.Furusawa, J.Soejima, H.Komatsu, H.Fukui
    • Journal Title

      Acta Horticulturae 622

      Pages: 389-396

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] S-allele genotypes of apple pollenizers and lineages including those resistant to scab.2003

    • Author(s)
      S.Matsumoto, Y.Furusawa, H.Komatsu, J.Soejima
    • Journal Title

      J.Hortic.Sci.Biotechnol 78

      Pages: 634-637

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Partial genomic sequences of S_<6^->, S_<12^->, S_<13^->, S_<14^->, S_<17^->, S_<19^->, and S_<21^->-RNases of apple and their allele designations2003

    • Author(s)
      S.Matsumoto, Y.Y.Furusawa, K.Kitahara, J.Soejima
    • Journal Title

      Plant Biotechnology 20

      Pages: 323-329

    • NAID

      10012536444

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Sequence of the S_<10>ic cDNA from ‘McIntosh' apple and a PCR-digestion identification method.2002

    • Author(s)
      K.Kitahara
    • Journal Title

      HortScience 37

      Pages: 187-190

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cloning of the S_<25> cDNA from ‘McIntosh' apple and an S_<25>-allele identification method.2002

    • Author(s)
      K.Kitahara
    • Journal Title

      J.Hortic.Sci.Biotechnol. 77

      Pages: 724-728

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Sequence of the S_<10> cDNA from 'McIntosh' apple and a PCR digestion identification method.2002

    • Author(s)
      K.Kitahara, S.Matsumoto
    • Journal Title

      HortScience 37

      Pages: 187-190

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cloning of the S_<25> cDNA from 'McIntosh' apple and an S_<25>-allele identification method.2002

    • Author(s)
      K.Kitahara, S.Matsumoto
    • Journal Title

      J.Hortic.Sci.Biotechnol. 77

      Pages: 724-728

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] MADS-box genes in rose : expression analyses of AGAMOUS, PISTILLATA, APETALA3 and SEPALLATA homologue genes in green rose.

    • Author(s)
      S.Matsumoto
    • Journal Title

      Acta Horticulturae (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] MADS-box genes in rose : expression analyses of AGAMOUS, PISTILLATA, APETALA3 and SEPALLATA homologue genes in gram rose.

    • Author(s)
      S.Matsumoto, K.Kitahara
    • Journal Title

      Acta Horticulturae (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] MADS-box genes in rose : expression analyses of AGAMOUS, PISTILLATA, APETALA3 and SEPALLATA homologue genes in green rose

    • Author(s)
      S.Matsumoto
    • Journal Title

      Acta Horticulturae (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Parent identification of eight apple cultivars by S-RNase analysis and simple sequence repeat markers

    • Author(s)
      K.Kitahara
    • Journal Title

      HortScience (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genomic DNA sequence of S_<16c(=16)>-RNase in apple : re-numbering of S_<16(=27a)>- and S_<22(=27b)>-allele to S_<16a> and S_<16b>

    • Author(s)
      S.Matsumoto
    • Journal Title

      Sci.Rep.Fac.Educ., Gifu Univ.(Nat.Sci.) (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Development of a teaching material for photography of flowers in ultraviolet

    • Author(s)
      S.Matsumoto
    • Journal Title

      Sci.Rep.Fac.Educ., Gifu Univ.(Nat.Sci.) (印刷中)

    • NAID

      110004470255

    • Related Report
      2004 Annual Research Report
  • [Publications] S.Matsumoto: "S-allele genotype of apple cultivars and selections"Acta Horticulturae. 622. 389-396 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Matsumoto: "S-allele genotype of apple pollenizers, cultivars and lineage including resistant to scab"Journal of Horticultural Science & Biotechnology. 78・5. 634-637 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Matsumoto: "Photography of flowers of spring seasons in ultraviolet using digital camera as a teaching material in science education"Sci.Rep.Fac.Educ., Gifu Univ.(Nat.Sci.). 28・1. 9-15 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Matsumoto: "Partial genomic sequences of S_6-, S_<12>-, S_<13>-, S_<14>-, S_<17>-, S_<19>- and S_<21>-RNase of apple and their allele designations"Plant Biotechnology. 20・4. 323-329 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Kitahara: "Cloning and characterization of apple class B MADS-box genes including a novel AP3 homologue 'MdTM6'"J.Japan Soc.Hort.Sci.. 印刷中.

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Kitahara: "Ectopic expression of the rose AGAMOUS-like MADS-box genes 'MASAKO C1 and D1' causes similar homeotic transformation of sepal and petal in Arabidopsis"Plant Science. 印刷中.

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Matsumoto: "S-allele genotype of apple cultivars and selections"Acta Horticulturae. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Kitahara: "Cloning of the S_<25> cDNA from 'McIntosh' apple and an S_<25>-allele identification method"Journal of Horticultural Science & Biotechnology. 77・6. 724-728 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Matsumoto: "Development of a teaching material for photosynthesis reaction"Sci. Rep. Fac. Educ. Gifu Univ. (Nat. Sci.). 27・1. 15-21 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Kitahara: "Sequence of the S_<10> cDNA from 'McIntosh' apple and a POR-digestion identification method"HortScience. 37・1. 187-190 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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