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Molecular mechanism of trans-translation reaction

Research Project

Project/Area Number 14360044
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionHirosaki University

Principal Investigator

HIMENO Hyouta  Hirosaki University, Faculty of Agriculture and Life Science, Associate Professor, 農学生命科学部, 助教授 (80208785)

Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2004: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2003: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2002: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordstmRNA / trans-translation / SmpB / translation / ribosome / aminoglycoside / tmRNA / パロモマイシン / アミノアシル化
Research Abstract

Trans-translation is an unusual translation in which tmRNA plays a dual function as a tRNA and an mRNA to relieve the stalled translation on the ribosome. In this study, we examined the effects of several kinds of aminoglycoside antibiotics, on trans-translation in vitro. A chemical footprinting study revealed that paromomycins bind tmRNA in the tRNA domain and in the middle of the long helix between tRNA and mRNA domains. Paromomycin bound in the tRNA domain inhibited aminoacylation, and the inhibition was suppressed by the addition of SmpB, a tmRNA binding protein. It was also found that paromomycin causes a shift of the translation-resuming point on tmRNA by -1. The effect on initiation-shift was suppressed by a mutation at the paromomycin binding site in 16S rRNA, but not by mutations in tmRNA. The effect of paromomycin on transtranslation differs substantially from that on canonical translation, in which it induces miscoding by modulating the A site of the decoding helix of the small subunit RNA of the ribosome. A minimal structure that causes initiation-shift was identified. The effect of each aminoglycoside on trans-translation coincides with that on conformational change in the A site of the small subunit of the ribosome revealed by recent structural studies : paromomycin, tobramycin and genetic in that is categorized into the gentamicin subclass, but not streptomycin and hygromycin B, flip out two conserved adenine bases at 1492 and 1493 from the A site helix.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (30 results)

All 2004 2003 2002 Other

All Journal Article (18 results) Book (2 results) Publications (10 results)

  • [Journal Article] 翻訳と共役した蛋白質品質管理システム:トランストランスレーション2004

    • Author(s)
      姫野俵太, 武藤
    • Journal Title

      細胞における蛋白質の一生(蛋白質核酸酵素増刊) 49(7)

      Pages: 1062-1066

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A minimum structure of aminoglycosides that causes an initiation shift of trans-translation.2004

    • Author(s)
      Konno, T., Takahashi, T., Kurita, D., Muto, A., Himeno, H.
    • Journal Title

      Nucleic Acids Res. 32

      Pages: 4119-4126

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel GTPase activated by the small subunit of ribosome.2004

    • Author(s)
      Himeno, H., Hanawa-Suetsugu, K., Kimura, T., Takagi, K., Sugiyama, W., Shirata, S., Mikami, T., Odagiri, F., Osanai, Y., Watanabe, D., Goto, S., Kalachnyuk, L., Ushida, C., Muto, A.
    • Journal Title

      Nucleic Acids Res. 32

      Pages: 5303-5309

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Initiation shift of trans-translation by aminoglycosides.2004

    • Author(s)
      Konno, T., Kurita, D., Takahashi, T., Muto, A., Himeno, H.
    • Journal Title

      Nucleic Acids Symp.Ser. 48

      Pages: 299-300

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Trans-translation : Co-translational protein quality control system.2004

    • Author(s)
      Muto, A., Himeno, H.
    • Journal Title

      Life of Proteins : Synthesis, Maturation : Traffic, Quality Control, Degradation, Disease (Protein, Nucleic Acid and Enzyme suppl) 49(7)

      Pages: 1062-1066

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 翻訳と共役した蛋白質品質管理システム:トランストランスレーション2004

    • Author(s)
      姫野俵太, 武藤 あきら
    • Journal Title

      細胞における蛋白質の一生(蛋白質核酸酵素増刊) 49(7)

      Pages: 1062-1066

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A minimum structure of aminoglycosides that causes an initiation shift of trans-translation.2004

    • Author(s)
      Konno, T., Takahashi, T., Kurita D., Muto, A., Himeno, H.
    • Journal Title

      Nucleic Acids Res. 32

      Pages: 4119-4126

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Various effects of paromomycin on tmRNA-directed trans-translation.2003

    • Author(s)
      Takahashi, T., Konno, T., Muto, A., Himeno, H.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 27672-27680

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Various effects of paromomycin on tmRNA-mediated trans-translation.2003

    • Author(s)
      Konno, T., Takahashi, T., Muto, A., Himeno, H.
    • Journal Title

      Nucleic Acids Res.Supplement 3

      Pages: 236-236

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro analysis of the initial steps of trans-translation.2003

    • Author(s)
      Takada, K., Hori-Takemoto, C., Kawazoe, M., Lee, S., Shirouzu, M., Yokoyama, S., Muto, A., Himeno, H.
    • Journal Title

      Nucleic Acids Res.Supplement 3

      Pages: 287-288

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Various effects of paromomycin on tmRNA-mediated trans-translation.2003

    • Author(s)
      Konno, T., Takahashi, T., Muto, A., Himeno, H.
    • Journal Title

      Nucleic Acids Res.Sup. 3

      Pages: 235-236

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro analysis of the initial steps of trans-translation.2003

    • Author(s)
      Takada, K., Hori-Takemoto, C., Kawazoe, M., Lee, S., Shirouzu, M., Yokoyama, S., Muto, A., Himeno, H.
    • Journal Title

      Nucleic Acids Res.Sup. 3

      Pages: 287-288

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Detection of tmRNA-mediated trans-translation products in Bacillus subtilis.2002

    • Author(s)
      Fujihara, A., Tomatsu, H., Inagaki, S., Tadaki, T., Ushida, C., Himeno, H., Muto, A.
    • Journal Title

      Genes to Cells 7

      Pages: 343-350

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] SmpB functions in various steps of trans-translation.2002

    • Author(s)
      Hanawa-Suetsugu, K., Takagi, M., Inokuchi, H., Himeno, H., Muto, A.
    • Journal Title

      Nucleic Acids Res. 30

      Pages: 1620-1629

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Trans-translation mediated by Bacillus subtilis tmRNA.2002

    • Author(s)
      Ito, K., Tadaki, T., Lee, S., Takada, K., Muto, A., Himeno, H.
    • Journal Title

      FEBS Lett. 516

      Pages: 245-252

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] tmRNAによるトランス翻訳機構と生理的機能2002

    • Author(s)
      武藤, 姫野俵太
    • Journal Title

      RNAの細胞生物学(蛋白質核酸酵素増刊) 48(4)

      Pages: 346-356

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Trans-translation mediated by Bacillus subtilis tmRNA.2002

    • Author(s)
      Ito, K., Tadaki, T., Lee, S., Takada, K., Mute, A., Himeno, H.
    • Journal Title

      FEBS Lett. 516

      Pages: 245-252

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular mechanism and physiological roles of trans-translation mediatedby tmRNA2002

    • Author(s)
      Muto, A., Himeno, H.
    • Journal Title

      RNA Biology of the Cell (Protein, Nucleic Acid and Enzyme suppl) 48(4)

      Pages: 346-356

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 命化学II-遺伝子の働きとその応用-2003

    • Author(s)
      渡辺公綱, 姫野俵太
    • Total Pages
      255
    • Publisher
      丸善
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Chemistry of life -Roles and application of the gene-2003

    • Author(s)
      Watanabe, K., Himeno, H.
    • Total Pages
      256
    • Publisher
      Maruzen
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Takahashi, T., Konno, T., Muto, A., Himeno, H.: "Various effects of paromomycin on tmRNA-directed trans-translation"J.Biol.Chem.. 278. 27672-27680 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Konno, T., Takahashi, T., Muto, A., Himeno, H.: "Various effects of paromomycin on tmRNA-mediated trans-translation"Nucleic Acids Res. Supplement. 3. 235-236 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takada, K., Hori-Takemoto, C., Kawazoe, M., Lee, S., Shirouzu, M., Yokoyama, S., Muto, A., Himeno, H.: "In vitro analysis of the initial steps of trans-translation"Nucleic Acids Res. Supplement. 3. 287-288 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 姫野俵太: "タンパク質がわかる"竹縄忠臣編(羊土社). 10 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujihara, A., Tomatsu, H., Inagaki, S., Tadaki, T., Ushida, C., Himeno, H., Muto, A.: "Detection of tmRNA-mediated trans-translation products in Bacillus subtilis"Genes to Cells. 7. 343-350 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hanawa-Suetsugu, K., Takagi, M., Inokuchi, H., Himeno, H., Muto, A.: "SmpB functions in various steps of trans-translation"Nucleic Acids Res.. 30. 1620-1629 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ito, K., Tadaki, T., Lee, S., Takada, K., Muto, A., Himeno, H.: "Trans-translation mediated by Bacillus subtilis tmRNA"FEBS Lett.. 516. 245-252 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takada, K., Hanawa, K., Lee, S., Himeno, H., Muto, A.: "The structure and function of tmRNA"Nucleic Acids Res.Supplement. 2. 65-66 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 武藤, 姫野俵太: "tmRNAによるトランス翻訳と生理的機能"蛋白質核酸酵素. 48・4. 346-356 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 渡辺公綱, 姫野俵太: "生命化学II -遺伝子の働きとその応用-"丸善. 255 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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