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Functional analysis of chromatin remodeling complezes and histone modifiers

Research Project

Project/Area Number 14360048
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionThe University of Tokyo

Principal Investigator

KATO Shigeki  The University of Tokyo, Institute Molecular and Cellular Biosciences, Professor, 分子細胞生物学研究所, 教授 (60204468)

Co-Investigator(Kenkyū-buntansha) TAKEYAMA Ken-ichi  The University of Tokyo, Institute Molecular and Cellular Biosciences, Associate Professor, 分子細胞生物学研究所, 助手 (30323570)
北川 浩史  東京大学, 分子細胞生物学研究所, 助手 (20345234)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2003: ¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 2002: ¥7,300,000 (Direct Cost: ¥7,300,000)
KeywordsTranscription / Chromatin remodeling / Co-regulator / Nuclear receptor / Vitamin D / 染色体構造調節因子複合体 / in vitro転写系 / クロマチンヌクレオソーム再構成系 / 細胞種特異的構成因子 / p160、p68 / ファミリー / 男性ホルモンレセプター / リガンド依存的 / 哺乳類特異的染色体構造調節因子 / ゲノム発現制御複合体 / クロマチンヌクレオソーム / cDNAスクリーニング / 細胞種特異的複合体構成因子 / ゲノム発現制御 / 転写制御因子
Research Abstract

We have been studied the function of nuclear receptors in terms of gene regulations. In the present study, to reveal the molecular basis of the ligand-induced transactivation of nuclear receptors, nuclear, mplexes associated with nuclear receptors have been biochemically purified. The complexes include co-repressor, co-activator and chromatin remodeling ones. Among them, we have extensively investigated the function of one of chromatin remodeling complexes, WINAC. WINAC appears to associate with VDR to bring it to the target gene promoters through reorganization of surrounding nucleosome arrays. Upon ligand binding, the other classes of co-regulator complexes are recruited to VDR/WINAC in the gene promoters.
We have been studied the function of nuclear receptors in terms of gene regulations. In the present study, to reveal the molecular basis of the ligand-induced transactivation of nuclear receptors, nuclear, mplexes associated with nuclear receptors have been biochemically purified. The complexes include co-repressor, co-activator and chromatin remodeling ones. Among them, we have extensively investigated the function of one of chromatin remodeling complexes, WINAC. WINAC appears to associate with VDR to bring it to the target gene promoters through reorganization of surrounding nucleosome arrays. Upon ligand binding, the other classes of co-regulator complexes are recruited to VDR/WINAC in the gene promoters.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Sato, T., Matsumoto, T., Kawano, H., Kato, S., et al.: "Brain masculinization requires androgen receptor function"Proc.Natl.Acad.Sci.USA. 101. 1673-1678 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Murayama, A., Kim, M., Yanagisawa, J., Kato, S., et al.: "Transrepression by a liganded nuclear receptor via a bHLH activator through co-regulator switching"EMBO J.. 23. 1598-1608 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kitagawa, H., Fujiki, R., Yoshimura, K., Kato, S., et al.: "The chromatin remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams Syndrome"Cell. 113. 905-917 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohtake, F., Takeyama, K., Matsumoto, T., Kato, S., et al.: "Modulation of estrogen receptor signalin by association with the activated dioxin receptor"Nature. 423. 545-550 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzawa, M., Takada, I., Yanagisawa, J., Kato, S., et al.: "Cytokines suppress adipogenesis and PPAR-gamma function through the TAK1/TAB1/NIK cascade"Nature Cell Biol.. 5. 224-230 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawano, H., Sato, T., Yamada, T., Kato, S., et al.: "Suppressive function of androgen receptor in bone resorption"Proc.Natl.Acad.Sci.USA. 100. 9416-9421 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, T., Matsumoto, T., Kawano, H., Watanabe, T., Uematsu, Y., Sekine, K., Fukuda, T., Aihara, K., Krust, A., Yamada, T., Nakamichi, Y., Yamamoto, Y., Nakamura, T., Yoshimura, K., Yoshizawa, T., Metzger, D., Chambon, P., Kato.S.: "Brain masculinization requires androgen receptor function."Proc.Natl.Acad.Sci.USA. 101. 1673-1678 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Murayama, A., Kim, M., Yanagisawa, J., Takeyama, K., Kato, S.: "Transrepression by a liganded nuclear receptor via a bHLH activator through co-regulator switching."EMBO J.. 23. 1598-1608 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kitagawa, H., Fujiki, R., Yoshimura, K., Mezaki, Y., Uematsu, Y., Matsui, D., Ogawa, S., Unno, K., Okubo, M., Tokita, A., Nakagawa, T., Ito, T., Ishimi, Y., Nagasawa, H., Matsumoto, T., Yanagisawa, J., Kato S: "The chromatin remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams Syndrome."Cell. 113. 905-917 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohtake, F., Takeyama, K., Matsumoto, T., Kitagawa, H., Yamamoto, Y., Nohara, K., Tohyama, C., Krust, A., Mimura, J., Chambon, P., Yanagisawa, J., Fujii-Kuriyama, Y., Kato S.: "Modulation of estrogen receptor signalin by association with the activated dioxin receptor."Nature. 423. 545-550 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzawa, M., Takada, I., Yanagisawa, J., Ohtake, F., Ogawa, S., Yamauchi, T., Kadowaki, T., Takeuchi, Y., Shibuya, H., Gotoh, Y., Matsumoto, K., Kato.S.: "Cytokines suppress adipogenesis and PPAR-gamma function through the TAKI/TAB 1/NIK cascade."Nature Cell Biol. 5. 224-230 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawano, H., Sato, T., Yamada, T., Matsumoto, T., Sekine, K., Watanabe, T., Nakamura, T., Fukuda, T., Yoshimura, K., Yoshizawa, T., Aihara, K., Yamamoto, Y., Nakamichi, Y., Metzger, D., Chambon, P., Nakamura, K., Kawaguchi, H., Kato.S.: "Suppressive function of androgen receptor in bone resorption."Proc.Natl.Acad.Sci.USA. 100. 9416-9421 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, T., Matsumoto, T., Kawano, H., Kato, S., et al.: "Brain masculinization requires androgen receptor function"Proc.Natl.Acad.Sci.USA. 101. 1673-1678 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Murayama, A., Kim, Yanagisawa, J., Kato.S., et al.: "Ligand-induced transrepression by a nuclear receptor mediated by a bHLH-type activator through co-regulator switching"EMBO J.. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kitagawa, H., Fujiki, R., Yoshimura, K., Kato.S., et al.: "The chromatin remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams Syndrome"Cell. 113. 905-917 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohtake, F., Takeyama, K., Matsumoto, T., Kato.S., et al.: "Modulation of estrogen receptor signalin by association with the activated dioxin receptor"Nature. 423. 545-550 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzawa, M., Takada, I., Yanagisawa.J., Kato.S., et al.: "Cytokines suppress adipogenesis and PPAR-gamma function through the TAK1/TAB1/NIK cascade"Nature Cell Biol.. 5. 224-230 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawano, H., Sato, T., Yamada, T., Kato, S., et al.: "Suppressive function of androgen receptor in bone resorption"Proc.Natl.Acad.Sci.USA. 100. 9416-9421 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzawa, M., Takada, I., Kato S., et al.: "Inhibition of adipogenesis by cytokines with suppression PPARγ function through the TAK1/TAB1-NIK mediated cascade"Nature Cell Biol.. 5. 224-230 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakamichi, Y., Shukunami, C., Kato S., et al.: "Chondromodulin-I (ChM-I) is a bone remodeling factor"Mol. Cell. Biol.. 23. 636-644 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sato, T., Matsumoto, T., Kato S., et al.: "Late onset of obesity in male androgen receptor-deficient (ARKO) mice"Biochem. Biophys. Res. Commun.. 300. 167-171 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yanagisawa, J., Kitagawa, H., Kato, S., et al.: "Nuclear receptor function requires a TFTC-type histone acetyl transferase complex"Mol. Cell. 9. 553-562 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takeyama, K., Ito, S., Kato, S., et al.: "Androgen-dependent neurodegeneration by polyglutamine-expanded human androgen receptor in drosophila"Neuron. 35. 855-864 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kitagawa, H., Yanagisawa, J., Kato, S., et al.: "Ligand selective potentiation of rat mineralocorticoid receptor activation function-1 (AF-1) by a CBP-containing HAT complex"Mol. Cell. Biol.. 22. 3698-3706 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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