| Project/Area Number |
14360049
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ISHII Masaharu The University of Tokyo, Graduate School of Agriculture and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (30193262)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥16,300,000 (Direct Cost: ¥16,300,000)
Fiscal Year 2003: ¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 2002: ¥8,900,000 (Direct Cost: ¥8,900,000)
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| Keywords | Reductive TCA cycle / 3-Hydroxynronionate cycle / Hydrogenobacter thermophils / Acidionus brierleyi / Hydrogenobacter / 3-ヒドロキシプロビオン酸サイクル / Acidianus / Hydrogenobactor thermophilus |
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| Research Abstract |
I succeeded in demonstrating in vitro carbon dioxide fixation reactions of both Pyruvate : ferredoxin oxidoreductase and 2-Oxoglutarate : ferredoxin oxidoreductase, which are operative within Hydrogenobactei-thermophilus as key enzymes of reductive TCA cycle. Both enzymes are reversible. Therefore, during the experiment of one enzyme, the other enzyme was used for the regeneration of reduced ferredoxin. Also, isocitrate dehydrogenase reaction was analyzed in detail, biotinylated protein was shown to be essential for the carbon dioxide fixation reaction.
Acyl-CoA carboxylase was also purified and characterized from a thermophilic sulfur oxidizing archeon, Acidianus brierieyi, which was a first example from archaea. The purified enzyme shared common characteristics with another acetyl-CoA carboxylases. Still, it had several characteristic differences from another acetyl-CoA carboxylases.
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