Analysis of microbial metabolism of biosulfur compounds and adaptive evolution for the degradation of xenobiotics
| Project/Area Number |
14360050
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
応用微生物学・応用生物化学
|
|---|
| Research Institution | Shibaura Institute of Technology (2003)
The University of Tokyo (2002) |
|---|
Principal Investigator |
OMORI Toshio Shibaura Institute of Technology, Department of Applied Chemistry, Prof., 大学院・工学研究科, 教授 (20011984)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
野尻 秀昭 東京大学, 生物生産工学研究センター, 助教授 (90272468)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2003: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2002: ¥8,800,000 (Direct Cost: ¥8,800,000)
|
|---|
| Keywords | Dimethyl Sulfide / Dibenzothiophene / Organic sulfur compounds / Dimethyl Sulfone / sigma54 / Sulfate starvation / Dimethyl Slufide / Dimethyl Slufone |
|---|
| Research Abstract |
Microbial dimethyl sulfide (DMS) conversion is thought to be involved in the global sulfur cycle. We isolated Pseudomonas putida strain DS1 from soil as a bacterium utilizing DMS as a sole sulfur source, and tried to elucidate the DMS conversion mechanism of strain DS1 at biochemical and genetic level. Strain DS1 oxidized DMS to dimethyl sulfone ( DMSO_2) via dimethyl sulfoxide, whereas the oxidation was repressed in the presence of sulfate, suggesting that a sulfate starvation response is involved in DMS utilization by starin DS1. Two of the five DMS-utilization-defective mutants isolated by transposon5 (Th5) mutagenesis had a Tn5 insertion in the ssuEAECBF operon, which has been reported to encode a two-component monooxygenase system (SsuED), an ABC-type transporter (SsuABC), and a small protein (SsuF), and also to play a key role in utilization of sulfonates and sulfate esters in another bacterium, P.pulida strain S-313. Disniption of ssuD and SsuD enzymatic activity demonstrated that methanesulfonate is a metabolic intermediate of DMS and desulfonated by SsuD.Disruption of ssuC cr ssuF also led to a DMS-utilization-defective phenotype. Another two mutants bad a defect in a gene homologous to pa2354 from P.aeruginosa PA01, which encodes a putative transcriptional regulator, while the remaining mutant had a defect in cysM encoding O-acetylserine(thio)-lyase B.
|
Report
(3 results)
Research Products
(2 results)
