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Coupling of Carbon and Nitrogen Regulation in Amino acid and Polyamine Catabolism through Two-component Regulatory Systems and A Novel Mechanism of Bacterial Catabolite Control

Research Project

Project/Area Number 14360060
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionAkita Research Institute of Food and Brewing (2004)
National Food Research Institute (2002-2003)

Principal Investigator

ITOH Yoshifumi  Akita Research Institute of Food and Brewing, Director General, 所長 (70135127)

Co-Investigator(Kenkyū-buntansha) KIMURA Keitarou  National Food Research Institute, Dep. of Appll. Microbiol., Senior Researcher, 応用微生物部, 主任研究官 (20353980)
KAMIO Yoshiyuki  Tohoku University, Graduate School of Agricultural Science, Professor, 大学院・農学研究科, 教授 (00109175)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 2004: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2002: ¥4,500,000 (Direct Cost: ¥4,500,000)
KeywordsPseudomonas aeruginosa / Polyamine catabolism / Two-component regulatory system / Catabolite control / Nitrogen regulation / Glutamate metabolism / Oxidoreductase / Transcription regulation / 参加安元酵素 / 二成分制御遺伝子 / ヒスチジン代謝 / シグマ54 / 転写因子 / リン酸化 / アルギニン / ヒスチジン / 代謝オペロン / ポリアミン / プロリン
Research Abstract

Pseudomonas aeruginosa PAO1 utilizes histidine via a catabolic pathway encoded by the hutHUTIG operon as both carbon and nitrogen sources, and expression of this operon is regulated by both carbon and nitrogen. Succinate (the optimal carbon source and a catabolite repressor for this bacterium) partially suppressed expression of the hut operon (by about 25%). Ammonia (the optimal nitrogen source) alone had little effect on hut operon expression. However it powerfully (<5%) abrogated residual expression in the presence of succinate. The CbrA-CbrB and NtrB-NtrC two-component regulatory systems were essential for histidine utilization as a carbon source and as a nitrogen source, respectively. Succinate appeared to suppress hut operon transcription via the CbrA-CbrB system. On the contrary, the dicarboxylate seemed to enhance hut operon expression through the NtrB-NtrC system, allowing cells to use histidine as a nitrogen source. Ammonia (a nitrogen regulator) antagonized the NtrB-NtrC depe … More ndent activation of hut operon expression.
A cbrB null mutant spontaneously produced a high frequency of revertants (about 10^<-5>), that restored the ability to utilize histidine as a carbon source. We isolated a revertant and identified a mutation (a C to T change at nt 251) in ntrC. In the revertant expression of the hut operon was no longer subjected to catabolite repression by succinate and to ammonia repression in the presence of succinate. The CbrB and NtrC response regulators are phosphorylated by cognate CbrA and NtrB proteins depending on carbon and nitrogen status, respectively, and serve as transcriptional activators. The NtrC251 protein having an amino acid substitution of Ala-84 to Val might have an altered structure that mimics the phosphorylated (active)status of the protein, resulting in constitutive expression of the hut operon. The absence of catabolite repression of the hut operon by succinate in the ntrC251 mutant supports the notion that modulation of CbrB phosphorylation levels is a crucial point in control of the hut operon by the catabolite repressor, succinate. Thus, the present study disclosed a novel mechanism of bacterial catabolite control, in which a two-component regulatory system is involved in sensing carbon status in the environment and modulating expression of a catabolic pathway. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (24 results)

All 2004 2003 2002 Other

All Journal Article (15 results) Book (2 results) Publications (7 results)

  • [Journal Article] Roles and regulation of the glutamate racemase isogens, racE and yrpC, in Bscillus subtilis2004

    • Author(s)
      K.Kimura, L.-S.Phan Tran, Y.Itoh
    • Journal Title

      Microbiology 150

      Pages: 2911-2920

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Roles and regulation of the glutamate racemase isogens, racE and yrpC, in Bacillus subtilis2004

    • Author(s)
      K.Kimura, L.-S.Phan Tran, Y.Itoh
    • Journal Title

      Microbiology 150

      Pages: 2111-2120

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Arginine and polyamine catabolism2004

    • Author(s)
      Yoshifumi Itoh, Yuji Nakada
    • Journal Title

      Pseudomomas (Kluwer/Plenum Publishers) Volume 3

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Roles and regulation of the glutamate racemase isogens, racE and vrpC, in Bacillus subtilis2004

    • Author(s)
      K.Kimura, L.-S.Phan Tran, Y.Itoh
    • Journal Title

      Microbiology 150

      Pages: 2911-2920

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of Bacillus γ-glutamyltransferase and its involvement in the degradation of capsule poly-γ-glutamate2004

    • Author(s)
      K.Kimura, L.-S.Phan Tzan, I.Uchida, Y.Itou
    • Journal Title

      Microbiology 150

      Pages: 4115-4123

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Identification of the putrescine biosynthetic genes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway2003

    • Author(s)
      Yuji Nakada, Yoshifumi Itoh
    • Journal Title

      Microbiology 149

      Pages: 707-714

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of the purescine biosynthetic genes in Pseudomonas aeruginosa and characterizatiton of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway2003

    • Author(s)
      Yuji Nakada, Yoshifumi Itoh
    • Journal Title

      Microbiology 150

      Pages: 707-714

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Streptomycin-resistant (rpsL) or rifampicin-resistant (rpoB) mutant in Pseudomonas putida KH146-2 confers enhaanced tolerance to organic chemicals2002

    • Author(s)
      Keiichi Hosokawa, Nyun-Ho Park, Takashi Inaoka, Yoshifumi Itoh, Kozo Ochi
    • Journal Title

      Environmental Microbiology 3

      Pages: 703-712

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Divergent Structure and Regulatory Mechanism of proline Catabolic Systems : Characterization of the PutAP Proline Catabolic Operon of Pseudomonas aeruginosa PAO1 and its Regulation by PutR, an AraC/Xy1S Family Protein2002

    • Author(s)
      Yuji Nakada, Yoshifumi Itoh
    • Journal Title

      Journal of Bacteriology 184

      Pages: 5633-5640

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional Analysis an REgulation of the Divergent spuABCDEFGH-spul Operons for Polyaminine Uptake and Utilization in Pseudomonas aeruginosa2002

    • Author(s)
      Chung-Dar Lu, Yoshifumi Itoh, Yuji Nakada, Ying Jiang
    • Journal Title

      Journal of Bacteriology 184

      Pages: 3765-3773

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization and Regulation of the gbuA Gene Encoding Guanidinobutyrase in the Arginine Dehydrogenase Pathway of Pseudomonas aeruginosa PAO12002

    • Author(s)
      Yuji Nakada, Yoshifumi Itoh
    • Journal Title

      Journal of Bacteriology 184

      Pages: 3577-3584

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization and regulation of the gbuA gene encoding guanidinobutyrase in the arginine dehydrogenas pathway of Pseudomonas aeruginosa PAO12002

    • Author(s)
      Yuji Nakada, Yoshifumi Itoh
    • Journal Title

      Journal of Bacteriology 184

      Pages: 3577-3584

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional analysis of the divergent spuABCDEFGH- spuJ operons for polyamine uptake and utilization in Pseudomonas aeruginoa2002

    • Author(s)
      Chung-Dar Lu, Yoshifumi Itoh, Yuji Nakada, Ying Jiang
    • Journal Title

      Journal of Bacteriology 184

      Pages: 3765-3773

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Divergent structure and Regulatory mechanism of proline catabolic systems : characterizatIon of the putAP proline catabolic operon of Pseudomonas aeruginosa PAO1 and its regulation by PutR, an AraC/XylS family protein2002

    • Author(s)
      Yuji Nakada, Yoshifumi Itoh
    • Journal Title

      Journal of Bacteriology 184

      Pages: 5633-35640

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Streptomycin-resistant (rpsL) or Rifampicin-resistance (rpoB) mutation in Pseudomonas putida KH146-2 confers enhanced tolerance to organic chemicals2002

    • Author(s)
      Keiichi Hosokawa, Nyun-Ho Park, Takashi Inaoka, Yoshifumi Itoh, Kozo Ochi
    • Journal Title

      Environmental Microbiology 3

      Pages: 703-712

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Pseudomonas volume 3(分担執筆)2004

    • Author(s)
      Yoshifumi Itoh, Yuji Nakada
    • Total Pages
      31
    • Publisher
      Kluwer/Plenum Pblishers
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Pseudomonas volume 3(分担執筆)2004

    • Author(s)
      Yoshifumi Itoh, Yuji Nakada
    • Total Pages
      31
    • Publisher
      Kluwer/Plenum Publishers
    • Related Report
      2004 Annual Research Report
  • [Publications] Yuji Nakada, Yoshifumi Itoh: "Identification of the putrescine biosynthetic genes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway"Microbiology. 149. 707-714 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshifumi Itoh, Yuji Nakada: "Pseudomonas volume 3(分担執筆)"Kluwer/Plenum Publishers. 31 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yuji Nakada, Yoshifumi Itoh: "Identification of the putrescine biosynthetic enes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway"Microbiology. 149. 707-714 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Keiichi Hosokawa, Nyun-Ho Park, Takashi Inaoka, Yoshifumi Itoh, Kozo Ochi: "Streptomycin-resistant (rpsL) or rifampicin-resistant (rpoB) mutant in Pseudomonas putida KH146-2 confers enhaanced tolerance to organic chemicals"Environmental Microbiology. 4. 703-712 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yuji Nakada, Yoshifumi Itoh: "Divergent Structure and Regulatory Mechanism of Proline Catabolic Systems : Characterization of the putAP Proline Catabolic Operon of Pseudomonas aeruginosa PAO1 and its Regulation by PutR, and AraC/XylS Family Protein"Journal of Bacteriology. 184. 5633-5640 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chung-Dar Lu, Yoshifumi Itoh, Yuji Nakada, Ying Jiang: "Functional Analysis and REgulation of the Divergent spuABCDEFGH-spuI Operons for Polyaminine Uptake and Utilization in Pseudomonas aeruginosa"Journal of Bacteriology. 184. 3765-3773 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yuji Nakada, Yoshifumi Itoh: "Chratcterization and Regulation of the gbuA Gene Encoding Guanidinobutyrase in the Arginine Dehydrogenase Pathway of Pseudomonas aeruginosa PAO1"Journal of Bacteriology. 184. 3377-3384 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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