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PROTEIN CHEMISTRY ON SELF-POLLEN RECOGNITION SYSTEM IN PLANT

Research Project

Project/Area Number 14360066
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionNARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Principal Investigator

TAKAYAMA Seiji  NARA INSTITUTE OF SCIENCE AND TECHNOLOGY, GRADUATE SCHOOL OF BIOLOGICAL SCIENCES, ASSOCIATE PROFESSOR, バイオサイエンス研究科, 助教授 (70273836)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥15,900,000 (Direct Cost: ¥15,900,000)
Fiscal Year 2003: ¥7,900,000 (Direct Cost: ¥7,900,000)
Fiscal Year 2002: ¥8,000,000 (Direct Cost: ¥8,000,000)
Keywordsself-incompatibility / Brassica / SP11 / SRK / MLPK / NMR / receptor / kinase / 自家不和合性 / SLG / 立体構造 / S遺伝子
Research Abstract

1.Solution structure of the pollen ligand, SP11
Many flowering plants possess a self-incompatibility (SI) system to prevent inbreeding. In Brassica, self/non-self recognition in mating is established through S-haplotype specific interactions between stigmatic receptor kinase, SPK, and its pollen ligand, SP11. In this study, we determined the solution structure of the SP11 protein of the S_8-haplotype (S_8-SP11) by using NMR spectroscopy. It folds into an α/β sandwich structure that resembles those of plant defensins. Residues important for structural integrity are highly conserved among the allelic SP11s, suggesting the existence of a common folding pattern. Structure-based sequence alignment and homology modeling of allelic SP11s identified a hypervariable (HV) region, which is thought to form a loop that bulges out from the body of the protein that is amenable to solvent exposure. We suggest that the HV region could serve as a specific binding site for the stigma receptor.
2.Finding of MLPK, a possible component of the stigmatic receptor complex.
SI response in Brassica is initiated by haplotype-specific interactions between the pollen-bome ligand SP11 and its stigmatic receptor kinase SRK. This binding induces autophosphorylation of SRK, which is then thought to trigger a signaling cascade that leads to self-pollen rejection. A recessive mutation of the modifier (m) gene eliminates the SI response in stigma. Positional cloning of M has revealed that it encodes a membrane-anchored cytoplasmic serine/threonine protein kinase, designated MLPK. Transient expression of MLPK restores the ability of mm papilla cells to reject self-pollen, suggesting that MLPK is a positive mediator of Brassica SI signaling. The plasma membrane localization of MLPK suggests that it may function in the vicinity of SRK. MLPK, acting within the SRK receptor complex, is an attractive model for the future study of SI signaling.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] H.Shiba: "The dominance of alleles controlling self-incompatibility in Brassica pollen is regulated at the RNA level."Plant Cell. 14. 491-504 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Takayama: "Peptide signalling in plants."Curr.Opin.Plant Biol.. 5. 382-387 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Takayama: "Molecular mechanism of self-recognition in Brassica self-incompatibility."J.Exp.Bot.. 54. 149-156 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Kakizaki: "Linear Dominance Relationship among Four Class-II S Haplotypes in Pollen is Determined by the Expression of SP11 in Brassica Self-Incompatibility."Plant Cell Physiol.. 44. 70-75 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Shiba: "Genomic organization of the S-locus region of Brassica."Biosci.Biotech.Biochem.. 67. 622-626 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] G.Suzuki: "The S haplotypes lacking SLG in the genome of Brassica rapa."Plant Cell Rep.. 21. 911-915 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Iwano: "Immunohistochemical Studies on Translocation of Pollen S-haplotype Determinant in Self-incompatibility of Brassica rapa."Plant Cell Physiol.. 44. 428-436 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Watanabe: "Recent progresses on self-incompatibility research in Brassica species."Breeding Sci.. 53. 199-208 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Mishima: "Structure of the Male Determinant Factor for Brassica Self-incompatibility."J.Biol.Chem.. 278. 36389-36395 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Murase: "A Membrane-Anchored Protein Kinase Involved in Brassica Self-Incompatibility Signaling."Science. 303. 1516-1519 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Shiba: "Duplicated SP11 genes produce alternative transcripts in the S15 haplotype of Brassica oleracea."Genes Genet.Syst.. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 磯貝 彰: "植物は自分の花粉をどうやって見分けるか-アブラナ科植物の自家不和合性の分子機構"蛋白質核酸酵素. 47. 700-707 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Shiba et al.: "The dominance of alleles controlling self-incompatibility in Brassica pollen is regulated at the RNA level."Plant Cell. 14. 491-504 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Takayama, Y.Sakagami: "Peptide signalling in plants."Curr.Opin.Plant Biol.. 5. 382-387 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Takayama, A.Isogai: "Molecular mechanism of self-recognition in Brassica self-incompatibility."J.Exp.Bot.. 149-156 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Kakizaki et al.: "Linear dominance relationship among four class-II S haplotypes in pollen is determined by the expansion of SP11 in Brassica self-incompatibility."Plant Cell Physiol.. 44. 70-75 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Shiba et al.: "Genomic organization of the S-locus region of Brassica."Biosci.Biotech.Biochem.. 67. 622-626 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] G.Suzuki et al.: "The S haplotypes lacking SLG in the genome of Brassica rapa."Plant Cell Rep.. 21. 911-915 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Iwano et al.: "Immunohistochemical studies on translocation of pollen 5-haplotype determinant in self-incompatibility of Brassica rapa."Plant Cell Physiol.. 44. 428-436 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Watanabe et al.: "Recent progresses on self-incompatibility research in Brassica species."Breeding Sci.. 53. 199-208 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Mishima et al.: "Structure of the male determinant factor for Brassica self-incompatibility."J.Biol.Chem.. 278. 36389-36395 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Murase et al.: "A membrane-anchored protein kinase involved in Brassica self-incompatibility signaling."Science. 303. 1516-1519 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Shiba et al.: "Duplicated SP11 genes produce altemative transcripts in the S15 haplotype of Brassica oleracea."Genes Genet.Syst.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Isoga, S.Takayama: "Molecular mechanism of self-incompatibility in Brassica."Tanpakushitsu Kakusan Koso. 47. 700-707 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] G.Suzuki: "The S haplotypes lacking SLG in the genome of Brassica rapa."Plant Cell Rep.. 21. 911-915 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Iwano: "Immunohistochemical studies on translocation of pollen S-haplotype determinant in self-incompatibility of Brassica rapa."Plant Cell Physiol.. 44. 428-436 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Watanabe: "Recent progresses on self-incompatibility research in Brassica species."Breeding Sci.. 53. 199-208 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Mishima: "Structure of the male determinant factor for Brassica self-incompatibility."J.Biol.Chem.. 278. 36389-36395 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Murase: "A membrane-anchored protein kinase involved in Brassica self-incompatibility signaling."Science. 303. 1516-1519 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Takayama: "Peptide signalling in plants"Curr. Opin. Plant Biol.. 5. 382-387 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Shiba: "The dominance of alleles controlling self-incompatibility in Brassica pollen is regulated at the RNA level"Plant Cell. 14. 491-504 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Takayama: "Molecular mechanism of self-recognition in Brassica self-incompatibility"J. Exp. Bot.. 54. 149-156 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Kakizaki: "Linear dominance relationship among four class-II S haplotypes in pollen is determined by the expression of SP11 in Brassica self-incompatibility"Plant Cell Physiol.. 44. 70-75 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Shiba: "Genomic organization of the S-locus region of Brassica"Biosci. Biotechnol. Biochem.. 67. 622-626 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] G.Suzuki: "The S haplotypes lacking SLG in the genome of Brassica rapa"Plant Cell Rep.. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Iwano: "Immunohistochemical studies on translocation of pollen S-haplotype determinant in self-incompatibility of Brassica rapa"Plant Cell Physiol.. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] 磯貝 彰: "植物は自分の花粉をどうやって見分けるか-アブラナ科植物の自家不和合性の分子機構"蛋白質核酸酵素. 47(6). 700-707 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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