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Research on the rate-limitting Factors affecting the activities of photosynthesis in leaves of tree plants

Research Project

Project/Area Number 14360089
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 林学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KOBAYASHI Yoshichika  Kyushu University, Graduate School of Agriculture, Professor, 農学研究院, 教授 (90087594)

Co-Investigator(Kenkyū-buntansha) 三宅 親弘  九州大学, 農学研究院, 助教授 (80294289)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2004: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2003: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥8,500,000 (Direct Cost: ¥8,500,000)
KeywordsPhotosynthesis / CO_2 fixation / Electron Transport / Tree plants / Rate-limitting step / Carbon dooxide / 脂肪酸 / FAD遺伝子 / クスノキ / タバコ / 光呼吸 / トコフェロール / 生体膜
Research Abstract

Leaves of evergreen trees are characterized by the low activity of CO_2 uptake compared with leaves of deciduous trees and herbaceous plants. However, we found that there are no substantial differences among leaves of these plant species in the activities of electron transport (Je) and CO_2 assimilation (JCO_2), when the activities (Je and JCO_2) were compared with leaves containing similar levels of CO_2 at the carboxylation site in stroma (Cc). The results suggest that chloroplasts of evergreen tree leaves have high abilities of electron transport and CO_2 assimilation in spite of their very low activities of leaf photosynthesis evaluated as the activity of CO_2 uptake under illumination with saturating light in air containing 350 ppm CO_2.
We, therefore, measured the photosynthetic parameters of attached leaves such as the stomatal and mesophyll conductance to CO_2 diffusion (gs and gm), the CO_2 concentrations in intercellular spaces and stroma (Ci and Cc), and the fluxes of electrons consumed by photosynthesis and photorespiration (JCO_2 and JPR). After the measurement, membrane lipids were isolated from the same leaves as used for the experiments, and the fatty acid composition was determined. A linear relationship was observed between the net activity of photosynthesis (JCO_2) and the mesophyll conductance, indicating that leaf photosynthesis depends on the mesophyll conductance (gm). A good correlation was also found between gm and the relative content of α-linolenic acid (18:3), and between gm and the relative content of palmitic acid (16:0). On the basis of these observations, several possible factors limiting the diffusion of CO_2 in leaf tissue of evergreen trees including Eucalyptus plants were determined.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (10 results)

All 2004 2003 Other

All Journal Article (4 results) Publications (6 results)

  • [Journal Article] An analysis of the mechanism of the low-wave phenomenon of chlorophyll fluorescence.2004

    • Author(s)
      Tsuyama, M., Shibata, M., Kawazu, T., Kobayashi, Y.
    • Journal Title

      Photosynthesis Research 81

      Pages: 67-76

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Chlorophyll formation and photosynthetic activity in rice mutants with alterations in hydrogenation of the chlorophyll alcohol side chain.2004

    • Author(s)
      Shibata, M., Mikota, T., Yoshimura, A., Iwata, N., Tsuyama, M., Kobayashi, Y.
    • Journal Title

      Plant Science 166

      Pages: 593-600

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Accumulation of menaquinones with incompletely reduced side chains and loss of α-tocopherol in rice mutants with alternations in the chlorophyll side chain.2004

    • Author(s)
      Shibata, M., Tsuyama, M., Takami, T., Shimizu, H., Kobayashi, Y.
    • Journal Title

      Journal of Experimental Botany 55

      Pages: 1989-1996

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Leaf factors affecting the relationship between chlorophyll fluorescence and the rate of photosynthetic electron transport as determined from CO_2 uptake.2003

    • Author(s)
      Tsuyama, M., Shibata, M., Kobayashi, Y.
    • Journal Title

      Journal of Plant Physiology 160

      Pages: 1131-1139

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Publications] Tsuyama, M., Shibata M., Kobayashi Y.: "Leaf factors affecting the relationship between chlorophyll fluorescence and the rate of photosynthetic electron transport as determined from CO_2 uptake"J.Plant Physiol.. 160. 1131-1139 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shibata M. et al.: "Chlorophyll formation and photosynthetic activity in rice mutants with alterations in the hydrogenation of chlorophyll alcohol side chain"Plant Science. 166(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsuyama M. et al.: "An analysis of the mechanism of the low-wave phenomenon of chlorophyll fluorescence"Photosynthesis Res.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shibata et al.: "Suppression of α-tocopherol in rice mutant in geranylgeranyl-chlorophyll reductase"Plant Physiol and Biochemistry. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsuyama, M., Shibata M., Kobayashi, Y.: "Leaf factors affecting the relationship between chlorophyll fluorescence and the rate of photosynthetic electron transport as determined from CO2 uptake"J. Plant Physiology. (In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小林善親 (共著): "巨大クスノキの研究"大宰府顕彰会. 230 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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