| Project/Area Number |
14360101
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産学
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| Research Institution | KYUSHU UNIVERSITY (2003-2004)
Forestry and Forest Products Research Institute (2002) |
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Principal Investigator |
KONDO Tetsuo KYUSHU UNIVERSITY, Bio-Architecture Center, Professor, バイオアーキテクチャーセンター, 教授 (30202071)
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| Co-Investigator(Kenkyū-buntansha) |
TOIDA Toshihiko Chiba University, Graduate School of Pharmaceutical Sciences, Professor, 大学院・薬学研究院, 教授 (60163945)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥16,700,000 (Direct Cost: ¥16,700,000)
Fiscal Year 2004: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2003: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2002: ¥8,400,000 (Direct Cost: ¥8,400,000)
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| Keywords | biomacromolecular tracks / protoplast / fiber spinning / callose / order / top-down / カロース繊維 / 繊維構造 / カルシウムイオン |
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| Research Abstract |
A unique fiber spinning was found in protoplasts from birch leaves under an acidic culture medium in the presence of high concentration of Ca^<2+>. After expanding from 10 μm over 100 μm in diameter under the culture condition, the protoplast started secreting a gigantic fiber while moving in a spiral way. Real time video analyses elucidated that the orientation, rate and pattern of the motion was directed due to the inverse force of the fiber spinning. Moreover, observation using several microscopic methods accompanied with histochemical staining indicated that the fiber was composed of 400-500 nm wide (1→3)-β-glucan hollow sub-fibrils. This entire phenomenon may be a programed response against the stress imposed. The observations presented in addition to providing an understanding of the unique relationship between directed fiber secretion and the bottom-up fiber fabrication from nano to micro scales, could also offer a new insight designing bio-based fiber materials.
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