| Project/Area Number |
14360105
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
YAMAHA Etsuro Hokkaido Univ., Field Science Center for Northern Biosphere., Prof., 北方生物圏フィールド科学センター, 教授 (60191376)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIZAKI Goro Tokyo Univ.of Marine Science and Technology, Asso.Prof., 海洋科学部, 助教授 (70281003)
HIRAI Toshiro Teikyo Univ.of Sci.and Tech., Div.of Advance Science and Technology, Inst., 理工学部, 助手 (30238331)
ARAI Katsutoshi Hokkaido Univ., Grad.School of Fish.Sci., Prof., 大学院・水産科学研究科, 教授 (00137902)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2003: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 2002: ¥9,200,000 (Direct Cost: ¥9,200,000)
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| Keywords | embryogenesis / germ-line chimera / nos 1 / primordial germ cells / teleost fish / transgenic trout / vasa / GFP / nos 1 / TGF-β super family / vas |
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| Research Abstract |
1)The origin and migration route were investigated by histological section and whole mount in situ hybridization of vas mRNA at a probe in ice goby and floating goby (Gobioidei, Perciformes, Teleostei).
2)Primordial germ cells were visualized at the somitogenesis stages with GFP fluorescence in herring, goldfish, loach and ice goby embryos, when fertilized eggs of these species were injected with chimeric RNAs containing GFP coding region and zebrafish nos1 3'-untranslated region.
3)A technique to visualize live PGCs using chimeric RNAs containing GFP coding region and vasa 3'-untranslated region was also established.
4)When visualized PGCs at the somitogenesis stage were inter-temporally transplanted into blastoderm at the blastula stage, donor PGCs moved to genital anlage of the host embryo in con-specific transplantation.
5)In the case of inter-temporal, xeno-generic transplantation, for example from goldfish to loach, donor PGCs moved to the host genital anlage through the host migration route with the host PGCs. But, in some combination of xeno-generic transplantation, such as from loach to ice goby, donor PGCs could not moved to the host genital anlage.
6)A technique to generate live fry from intraperitoneally transplanted primordial germ cells was established in salmonid fish.
7)A technique to isolate highly pure and viable PGCs from rainbow trout by GFP-dependent flow cytometry was established.
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