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Developmental analyzes of primordial germ cells and induction of germ-line chimera in fish.

Research Project

Project/Area Number 14360105
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

YAMAHA Etsuro  Hokkaido Univ., Field Science Center for Northern Biosphere., Prof., 北方生物圏フィールド科学センター, 教授 (60191376)

Co-Investigator(Kenkyū-buntansha) YOSHIZAKI Goro  Tokyo Univ.of Marine Science and Technology, Asso.Prof., 海洋科学部, 助教授 (70281003)
HIRAI Toshiro  Teikyo Univ.of Sci.and Tech., Div.of Advance Science and Technology, Inst., 理工学部, 助手 (30238331)
ARAI Katsutoshi  Hokkaido Univ., Grad.School of Fish.Sci., Prof., 大学院・水産科学研究科, 教授 (00137902)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2003: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 2002: ¥9,200,000 (Direct Cost: ¥9,200,000)
Keywordsembryogenesis / germ-line chimera / nos 1 / primordial germ cells / teleost fish / transgenic trout / vasa / GFP / nos 1 / TGF-β super family / vas
Research Abstract

1)The origin and migration route were investigated by histological section and whole mount in situ hybridization of vas mRNA at a probe in ice goby and floating goby (Gobioidei, Perciformes, Teleostei).
2)Primordial germ cells were visualized at the somitogenesis stages with GFP fluorescence in herring, goldfish, loach and ice goby embryos, when fertilized eggs of these species were injected with chimeric RNAs containing GFP coding region and zebrafish nos1 3'-untranslated region.
3)A technique to visualize live PGCs using chimeric RNAs containing GFP coding region and vasa 3'-untranslated region was also established.
4)When visualized PGCs at the somitogenesis stage were inter-temporally transplanted into blastoderm at the blastula stage, donor PGCs moved to genital anlage of the host embryo in con-specific transplantation.
5)In the case of inter-temporal, xeno-generic transplantation, for example from goldfish to loach, donor PGCs moved to the host genital anlage through the host migration route with the host PGCs. But, in some combination of xeno-generic transplantation, such as from loach to ice goby, donor PGCs could not moved to the host genital anlage.
6)A technique to generate live fry from intraperitoneally transplanted primordial germ cells was established in salmonid fish.
7)A technique to isolate highly pure and viable PGCs from rainbow trout by GFP-dependent flow cytometry was established.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] 吉崎悟朗: "始原生殖細胞を用いた魚類の発生工学"BIO INDUSTRY. 21. 29-37 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi, T.: "Cryopreservation of trout primordial germ cells."Fish Physiol.Biochemist.. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kusuda, S.: "Pluripotency of cryopreserved blastomeres of the goldfish."J.Exp.Zool.. 301A. 131-138 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashimoto, Y.: "Requirement of localized maternal factors for zebrafish germ cell formation."Dev.Biol.. 268. 152-161 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi, T.: "Isolation of highly pure and viable primordial germ cells from rainbow trout by GFP-dependent flow cytometry."Mol.Reprod.Dev.. 67. 91-100 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshizaki, G.: "Primordial germ cells : A novel tool for fish bioengineering."Fish Physiol.Biochem.. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Boonanuntanasarn, S.: "Specific gene silencing using small interfering RNAS in fish embryos."Biochem.Biophys.Res.Comm.. 310. 1089-1095 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeuchi, Y.: "Generation of live fry from intraperitoneally transplanted primordial germ cells in rainbow trout."Biol Reprod. 69. 1142-1149 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaha, E.: "Recovery of fertility in male hybrids of a cross between goldfish and common carp by transplantation of PGCs (primordial germ cell)-containing graft."Genetica. 19. 121-131 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeuchi, Y.: "Mass isolation of primordial germ cells from transgenic rainbow trout carrying the GFP gene driven by the vasa gene promoter."Biol Reprod. 67. 1087-1092 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saito, T.: "Germ cell lineage from a single blastomere at 8-cell stage in Shiro-uo (ice goby)."Zoological Science. 19. 1027-1032 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Otani, S.: "The germ cell lineage identified by vas-mRNA during embryogenesis in goldfish."Zoological Science. 19. 519-526 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshizaki, G.: "Primordial germ cells : the blueprint for piscine life."Fish Physiol.Biochem.. 26. 3-12 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshizaki, G., Takeuchi, Y., Kobayashi, T., Tago, Y., Takeuchi, T.: "Fish bioengineering by primordial germ cells (in Japanese)"BIO INDUSTRY. 21. 29-37 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi, T., Takeuchi, Y., Yoshizaki, G., Takeuchi, T.: "Cryopreservation of trout primordial germ cells."Fish Physiol.Biochemist.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kusuda, S., Teranishi, T., Koide, N., Nagai, T., Arai, K., Yamaha, E.: "Pluripotency of cryopreserved blastomeres of the goldfish."J.Exp.Zool.. 301A. 131-138 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashimoto, Y., Maegawa, S., Nagai, T., Yamaha, E., Suzuki, H., Yasuda, K., Inoue, K.: "Requirement of localized maternal factors for zebrafish germ cell formation."Dev.Biol.. 268. 152-161 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi, T., Yoshizaki, G., Takeuchi, Y., Takeuchi, T.: "Isolation of highly pure and viable primordial germ cells from rainbow trout by GFP-dependent flow cytometry."Mol.Reprod.Dev.. 67. 91-100 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshizaki, G., Takeuchi, Y., Kobayashi, T., Takeuchi, T.: "Primordial germ cells : A novel tool for fish bioengineering."Fish Physiol.Biochem.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Boonanuntanasarn, S., Yoshizaki, G., Takeuchi, T.: "Specific gene silencing using small interfering RNAs in fish embryos."Biochem.Biophys.Res.Comm.. 310. 1089-1095 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeuchi, Y., Yoshizaki, G., Takeuchi, T.: "Generation of live fry from intraperitoneally transplanted primordial germ cells in rainbow trout."Biol.Reprod.. 69. 1142-1149 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaha, E., Murakami, M., Hada, K., Otani, S., Fujimoto, T., Tanaka, M., Sakao, S., Kimura, S., Sato, S., Arai, K.: "Recovery of fertility in male hybrids of a cross between goldfish and common carp by transplantation of PGCs (primordial germ cell)-containing graft."Genetica. 119. 121-131 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeuchi, Y., Yoshizaki, G., Kobayashi, T., Takeuchi, T.: "Mass isolation of primordial germ cells from transgenic rainbow trout carrying the green fluorescent protein gene driven by the vasa gene promoter."Biol.Reprod.. 67. 1087-1092 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saito, T., Otani, S., Nagai, T., Miyake, A., Nakatsuji, N., Nakatsuji, T., Arai, K., Yamaha, E.: "Germ-cell lineage from a single blastomere at 8-cell stage in shiro-uo."Zool.Sci.. 19. 1027-1032 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Otani, S., Maegawa, S., Inoue, K., Arai, K., Yamaha, E.: "The germ cell lineage identified by vas-mRNA during embryogenesis in goldfish."Zool.Sci.. 19. 519-526 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshizaki, G., Takeuchi, Y., Kobayashi, T., Ihara, S., Takeuchi, T.: "Primordial germ cells the blueprint for piscine life."Fish Physiol.Biochem.. 26. 3-12 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 吉崎悟朗: "始原生殖細胞を用いた魚類の発生工学"BIO INDUSTRY. 21. 29-37 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kobayashi, T.: "Isolation of highly pure and viable primordial germ cells from rainbow trouto by GFP-dependent flow cytometry."Mol.Reprod.Dev.. 67. 91-100 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kusuda, S.: "Pluripotency of cryopreserved blastomeres of the goldfish."J.exp.Zool.. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Hashimoto, Y.: "Requirement of localized maternal factors for zebrafish germ cell formation."Dev.Biol.. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Kobayashi, T.: "Cryopreservation of trout primordial germ cells."Fish Physiol.Biochemist. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshizaki, G.: "A novel tool for fish bioengineering."Fish Physiol.Biochemist.. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Saito, T.: "Germ cell lineage from a single blastomere at 8-cell stage in Shiro-uo(ice goby)"Zoological Science. 19. 1027-1032 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamaha, E.: "Sterile hybrids male as surrogate fathers : Recovery of fertility in male hybrids of a cross between goldfish and common carp by transplantation of goldfish graft, including PGCs"Genetica. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshizaki, G.: "Primordial germ cells : the blue print for a piscine life"Fish Physiol.Biochem. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Takeuchi, Y.: "Mass isolation of primordial germ cells from transgenic rainbow trout carrying the GFP gene driven by the vasa gene promoter"Biol Reprod. 67. 1087-1092 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Boonanuntanasarn, S.: "Gene knock-down in rainbow trout embryos using antisense morpholino phosphorodiamidate oligonucleotides"Marine Biotechnology. 4. 256-266 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshizaki, G.: "Visualization of primordial germ cells in transgenic rainbow trout carrying green fluorescent protein gene driven by vasa promoter"Fisheries Science. 68. 1067-1070 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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