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MOLECULAR ANALYSES OF FREE D-AMINO ACID METABOLIZING ENZYMES IN AQUATIC ORGANISMS

Research Project

Project/Area Number 14360121
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

ABE Hiroki  The University of Tokyo, GRADUATE SCHOOL OF AGRICULTUERL AND LIFE SCIENCES, PROFESSOR, 大学院・農学生命科学研究科, 教授 (80086727)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Masahiro  The University of Tokyo, GRADUATE SCHOOL OF AGRICULTUERL AND LIFE SCIENCES, ASSOCIATE PROFESSOR, 大学院・農学生命科学研究科, 助教授 (70134517)
OKADA Sigeru  The University of Tokyo, GRADUATE SCHOOL OF AGRICULTUERL AND LIFE SCIENCES, RESEARCH ASSOCIATE, 大学院・農学生命科学研究科, 助手 (00224014)
WATANABE Shugo  The University of Tokyo, GRADUATE SCHOOL OF AGRICULTUERL AND LIFE SCIENCES, PROFESSOR, 大学院・農学生命科学研究科, 教授 (40111489)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2003: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2002: ¥10,000,000 (Direct Cost: ¥10,000,000)
Keywordsfree D-amino acid / D-alanine / alanine racemase / D-amino acid oxidase / D-aspartate oxidase / isosmotic regulation / invertebrate / fish
Research Abstract

In order to understand the physiological functions of free D-alanine (Ala) found in large amount in aquatic crustaceans and some bivalve mollusks, we determined the primary structure of alanine race mase (ARase) catalyzed the interconversion between D-and L-Ala. ARase cDNA cloned, from the muscle and hepatopancreas of kuruma prawn Penaeus japon.icus for the first time in higher eukaryotes consisted of 1,798bp nucleotide sequence including the ORF encoding 421 amino acid residues.
cDNA of D-amino acid oxidase (DAO) was cloned from the hepatopancreas of common carp Cyprlnus carpio for the first time in animals other than mammals. The cDNA contained 1,294bp including the ORF of 1,041bp encoding 347 amino acid residues.. The carp DAO was expressed in E. coil cells and purified to homogeneity. The recombinant DAO showed the highest activity against D-Ala. The enzymatical and structural characteristics of carp DAO positioned it between pig kidney and yeast DAOs.
In carp fed the diet with D-Ala for 14days, DAO mRNA was strongly expressed in intestine followed by hepatopancreas and kidney, but not in muscle. The inducible nature of carp DAO indicates that it plays an important physiological role in metabolizing exogenous D-Ala that is abundant in their prey invertebrates, crustaceans and mollusks.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Fujimori T, Abe H: "Physiological roles of free D- and L-alanine in the crayfish Procambarus clarkii with special reference to osmotic and anoxic stress responses"Comp.Biochem.Physiol.. 131A. 895-902 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Abe H: "Distribution, metabolism, and physiological functions of free D-amino acids in aquatic invertebrates"Nippon Suisan Gakkaishi. 68. 516-525 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshikawa N, Dhomae N, Takio K, Abe H: "Purification, properties, and partial amino acid sequences of alanine racemase from the muscle of the black tiger prawn Penaeus monodon"Comp.Biochem.Physiol.. 133B. 445-453 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sarower MDG, Matsui T, Abe H: "Distribution and characterization of D-amino acid and D-aspartate oxidases in fish tissues"J.Exp.Zool.. 295A. 151-159 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sarower MDG, Okada S, Abe H.: "Molecular characterization of D-amino acid oxidase from common carp Cyprinus carpio and its induction with exogenous free D-alanine"Arch.Biochem.Biophys.. 420. 121-129 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujimori T, Abe H: "Physiological roles of free D-and L-alanine in the cray-fish Procanlbarus clarkil with special reference to osmotic and anoxic stress responses"Comp.Biochem.Physiol.. 131A. 895-902 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Abe H: "Distribution, metabolism, and physiological functions of free D-amino acids in aquatic invertebrates"Nippon Suisan Gakkaishi. 68. 516-525 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshikawa N, Dhomae N, Takio K, Abe H: "Purification, properties, and partial amino acid sequences of alanine racemase from the muscle of the black tiger prawn Penaeus inonodon"Comp.Biochem.Physiol. 133B. 445-453 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sarower MDG, Matsui T, Abe H: "Distribution and characterization of D-amino acid and D-aspartate oxiclases in fish tissues"J.Exp.Zool.. 295A. 151-159 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sarower MDG, Okada S, Abe H: "Molecular characterization of D-amino acid oxidase from common carp Cyprlnus carplo and its induction with exogenous free D-alanine"Arch.Biochem.Biophys.. 420. 121-129 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sarower, MD.G, S., Abe, H.: "Molecular characterization of D-amino acid oxidase from common carp Cyprinus carpio and its induction with exogenous free D-alanine"Archives of Biochemistry and Biophysics. 420. 121-129 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Abe, H.: "Alanine racemase and D-amino acid oxidase in aquatic animals"Amino Acids. 25. 135-136 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 阿部宏喜: "生体内D-アミノ酸研究の発展"蛋白質 核酸 酵素. 49. 87-89 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujimori, T.: "Physiological roles of free D-and L-alanine in the crayfish Procambarus clarkii with special reference to osmotic and anoxic stress responses"Comparative Biochemistry and Physiology. 131A. 893-900 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshikawa, N.: "Purification, properties, and partial amino acid sequences of alanine racemase from the muscle of black tiger prawn Penaeus monodon"Comparative Biochemistry and Physiology. 133B. 445-453 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 阿部宏喜: "水生無脊椎動物における遊離アミノ酸の分布,代謝および生理機能"日本水産学会誌. 68(4). 517-526 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 阿部宏喜: "水生無脊椎動物における遊離D-アラニンの生理機能"Viva Origino. 30(4). 221-228 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sarower, M.G.: "Distribution and characterization of D-amino acid and D-aspartate oxidases in fish tissues"Journal of Experimental Zoology. 295A. 151-159 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 阿部宏喜: "D-アラニンによるエビ・カニ類の環境適応戦略"化学と生物. 41(2). 72-74 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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