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Analyses for the regulation of mammalian oocyte/early embryo specific cell cycle which is aimed for introduction into somatic cells

Research Project

Project/Area Number 14360173
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionThe University of Tokyo

Principal Investigator

NAITO Hideaki  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (20188858)

Co-Investigator(Kenkyū-buntansha) CHIDA Kazuhiro  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (00192188)
AOKI Fugaku  The University of Tokyo, Graduate School of Frontier Sciences, Associate Professor, 大学院・新領域創成科学研究科, 助教授 (20175160)
TOJO Hideaki  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (20041668)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2003: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2002: ¥8,900,000 (Direct Cost: ¥8,900,000)
KeywordsMammalian ova / Meiosis / Early embryo development / Cell cycle / MPF / Rb / Mos / antisense RNA
Research Abstract

I analyzed the regulation mechanisms of mammalian oocytelearly embryo specific cell cycle, aiming for the introduction of their cell cycle into somatic cells. I found the following new mechanisms in oocyte meiosis (1, 2) and early embryo cleavage (3, 4).
(1)The large nucleus in immature oocyte, germinal vesicle (GV), was required for the meiosis specific consecutive two M-phases without S-phase, and the oocyte entered into S-phase after only one M-phase as somatic cells when GV was removed. This failure was attributed to the degradation of cyclin B during second meiosis.
(2)The suppression of MAPK activity by injection of anatisense-RNA of MAPK activator induced the significant increase of cyclin B accumulation in porcine oocytes.
(3)The accelerated cell cycle of early embryo cleavage was due to the absence of Rb protein, which inhibits the G1/S transition, in early embryos and, therefore, next S-phase started immediately after M-phase. The expression of Rb in early embryos by the injection of Rb-expression vector resulted the appearance of G1-phase after M-phase.
(4)The artificial activation or inhibition of MA.PK by injecting constitutively active or inactive mutant MAPK activator had no effect on the early embryo cleavage, suggesting the dispensability of MAPK for cleavage and the importance of Rb absence.
These findings might be useful for the induction of meiosis into somatic cells and production of haploid cells artificially.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Iwamori Naoki: "Preimplantation-embryo-specific cell cycle regulation is attributed to the low expression level of retinoblastoma protein."FEBS letter. 526. 119-123 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Goto Seitaro: "Effects of spindle removal on MPF and MAP kinase activities in porcine matured oocytes."Molecular Reproduction and Development. 63. 388-393 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohashi Satoshi: "Analyses of mitogen-activated protein kinase function in the maturation of porcine oocytes."Biology of Reproduction. 68. 604-609 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kuroda Takao: "Localization and Function of cyclin B1 and cyclin B2 during porcine oocyte maturation."Journal of Mammalian Ova Research. 20. 93-98 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kuroda Takao: "Analysis of the roles of cyclin B1 and cyclin B2 in porcine oocyte maturation by inhibiting synthesis with antisense RNA injection."Biology of Reproduction. 70. 154-159 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 内藤 邦彦: "動物生殖学:(受精と初期胚の発生 担当)(佐藤英明編)"朝倉書店、東京. 225 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iwamori N, Naito K, Sugiura K, Tojo: "Preimplantation-embryo-specific cell cycle regulation is attributed to the low expression level of retinoblastoma protein."FEBS letter. 526. 119-123 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Goto S, Naito K, Ohashi S, Sugiura K, Naruoka H, Iwamori N, Tojo H: "Effects of spindle removal on MPF and MAP kinase activities in porcine matured oocytes."Molecular Reproduction and Development. 63. 388-393 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohashi S, Naito K, Sugiura K, Iwamori N, Goto S, Naruoka H, Tojo H.: "Analyses of mitogen-activated protein kinase function in the maturation of porcine oocytes."Biology of Reproduction. 68. 604-609 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kuroda T, Naito K: "Localizationn and Function of cyclin B1 and cychn B2 during porcine oocyte maturation."Journal of Mammalian Ova Research. 20. 93-98 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kuroda T, Naito K, Sugiura K, Yamashita M, Takakura I, Tojo H: "Analysis of the roles of cyclin Bl and cyclin B2 in porcine oocyte maturation by inhibiting synthesis with antisense RNA injection."Biology of Reproduction. 70. 154-159 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Naito K: "Fertilization and development of early embryo (in Japanese Jusei to Syokihai no Hassei)"Doubutsu Seishokugaku (Asakura Shoten)(Sato E. ed). 225. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohashi Satoshi: "Analyses of mitogen-activated protein kinase function in the maturation of Porcine oocytes."Biology of Reproduction. 68. 604-609 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kuroda Takao: "Localization and Function of cyclin B1 and cyclin B2 during porcine oocyte maturation."Journal of Mammalian Ova Research. 20. 93-98 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Haraguchi Seiki: "Specific gene silencing in the pre-implantation stage mouse embryo by an siRNA expression vector system."Molecular Reproduction and Development. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kuroda Takao: "Analysis of the roles of cyclin B1 and cyclin B2 in porcine oocyte maturation by inhibiting synthesis with antisense RNA injection."Biology of Reproduction. 70. 154-159 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 内藤 邦彦: "動物生殖学:(受精と初期胚の発生 担当)"佐藤英明編、朝倉書店、東京. 225 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Iwamori Naoki: "Preimplantation-embryo-specific cell cycle regulation is attributed to the low expression level of retinoblastoma protein"FEBS letter. 526. 119-123 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sugiura Koji: "Activation of ribosomal S6 kinase (RSK) during porcine oocyte maturation"Zygote. 10. 31-36 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kikuchi Kazuhiro: "Maturation/M-phase promoting factor regulates aging of porcine oocytes matured in vitro"Cloning and Stem Cells. 4. 211-222 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 内藤 邦彦: "動物発生工学:(受精と初期発生;卵子の体外成熟;医療への応用 担当)"岩倉洋一郎・佐藤英明・舘鄰・東條英昭編、朝倉書店、東京. 270 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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