| Project/Area Number |
14370002
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Tohoku University |
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Principal Investigator |
KONDO Hisatake Tohoku University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (20004723)
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| Co-Investigator(Kenkyū-buntansha) |
OWADA Yuji Tohoku University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (20292211)
SAKAGAMI Hiroyuki Tohoku University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (90261528)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2003: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 2002: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | fatty acid binding protein / epidermal water barrier / radial glia / astrocyte / thymic epithclial cell / interleukine 12 / dendritic cell / 脂肪酸結合蛋白 / ラジアルグリア / E-FABP / B-FABP / H-FABP / 遺伝子欠損 / DHA / 記憶低下 |
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| Research Abstract |
Among fatty acid binding proteins (FABPs), the localization of brain(B)-and epidermal (E)-FABPs were examined in details in varous organs/tissues of mice, and gene knockout mice for the two FABPs were generated and their phenotypes were analysed in neuro-immune systems. While B-FABP was localized in the ventricular germinal zone and radial glial cells at prenatal stages and confined to the astrocytes in postnatal brain, E-FABP was localized in neurons and glia at the perinatal stage and confined to astrocytes in postnatal brain. While B-FABP was localized only in hepatic Kupffer cells, E-FABP was localized in splenic dendritic cells, peritoneal and alveolar macrophages, and thymic epithelical cells of adult mice. With the new advantage of E-FABP as a specific marker for the dendritc cells, the phagocytosis of lipopolysaccharide-induced apoptotic splenocytes in situ by the dendritic cells was first revealed. The basal transepidermal water loss of homozygous mice for E-FABP was lower than that of the wild mice. When the water permeability barrier of skin was disrupted by aceton application, recovery in transepidermal water loss was delayed, although no marked differences ware detected in ultrastructure and lipid contents of epidermis. Dendritic cells isolated from E-FABP-homozygous mice showed a marked decrease of IL-12 secretion. B-FABP-homozygous mice showed deterioration of learnining, memory anxiety.
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