• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Production and analyses of mice deficient in molecules involved in cell polarization

Research Project

Project/Area Number 14370003
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionGunma University

Principal Investigator

HARADA Akihiro  Gunma Univ., Inst.Mol.Cell.Regul., Professor, 生体調節研究所, 教授 (40251441)

Co-Investigator(Kenkyū-buntansha) HAYASHI Kensuke  Gunma Univ., Inst.Mol.Cell.Regul., Associate Professor, 生体調節研究所, 助教授 (50218567)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2004: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2003: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2002: ¥4,800,000 (Direct Cost: ¥4,800,000)
Keywordspolarized transport / cell polarity / epithelial cell / neuron / Cre-loxP / gene targeting / rab proteins / SNARE proteins / TGN / rab / SNARE / ノックアウトマウス / Cre / loxP
Research Abstract

Cell polarity is crutial for function of various types of cells. For cell polarity, polarized transport within cells are thought to be essential. A number of proteins (e.g, rab proteins and SNARE proteins) have been shown to be involved in polarized transport. However, their roles in the development and function of tissues and whole body of animals are largely unknown. Therefore, to uncover their roles in the development and function in the animals, we are making knockout mice for each of these proteins. As it is possible that these molecules are involved in embryonic development, simply knocking out of these genes may lead to embryonic lethality at very early stages of development. To circumvent this problem, we are making conditional knockout mice using Cre-loxP system.
During the term of grant, we have completed making conditional knockout mice of these proteins and are now analyzing their phenotypes. In addition, we analyzed AP3B knockout mice under electron microscopy and showed that the diameter of their synaptic vesicles became enlarged, which may lead to the neurological phenotype (convulsion) of this knockout mice. We also looked at the polarization process of neurons in vitro and observed that (1)axons were able to be formed from dendrites (2)axonogenesis is delayed in inhibitory neurons compared to excitatory neurons.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (17 results)

All 2004 2003 2002 Other

All Journal Article (13 results) Publications (4 results)

  • [Journal Article] Gem GTPase and tau : morphological changes induced by gem GTPase in cho cells are antagonized by tau2004

    • Author(s)
      Fumitaka Oyama
    • Journal Title

      The Journal of Biological Chemistry 279

      Pages: 27272-27277

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Defective function of GABA-containing synaptic vesicles in mice lacking the AP-3B clathrin adaptor2004

    • Author(s)
      Fubito Nakatsu
    • Journal Title

      The Journal of Cell Biology 167

      Pages: 293-302

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Gem GTPase and tau : morphological changes induced by gem GTPase in cho cells are antagonized by tau.2004

    • Author(s)
      Oyama F, Kotliarova S, Harada A, Ito M, Miyazaki H, Ueyama Y, Hirokawa N, Nukina N, Ihara Y.
    • Journal Title

      J.Biol.Chem. 279(26)

      Pages: 27272-27277

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Defective function of GABA-containing synaptic vesicles in mice lacking the AP-3B clathrin adaptor.2004

    • Author(s)
      Nakatsu F, Okada M, Mori F, Kumazawa N, Iwasa H, Zhu G, Kasagi Y, Kamiya H, Harada A, Nishimura K, Takeuchi A, Miyazaki T, Watanabe M, Yuasa S, Manabe T, Wakabayashi K, Kaneko S, Saito T, Ohno H.
    • Journal Title

      J.Cell Biol. 167(2)

      Pages: 293-302

    • NAID

      120004898419

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Defective function of GABA-containing synaptic vesicles in mice lacking the AP-3B clathrin adaptor2004

    • Author(s)
      Fubito Nakatsu
    • Journal Title

      The Journal of Cell Biology 167・2

      Pages: 293-302

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Gem GTPase and tau : morphological changes induced by gem GTPase in cho cells are antagonized by tau2004

    • Author(s)
      Fumitaka Oyama
    • Journal Title

      The Journal of Biological Chemistry 279・26

      Pages: 27272-27277

    • Related Report
      2004 Annual Research Report
  • [Journal Article] TDAG8 is a proton-sensing and psychosine-sensitive G-protein-coupled receptor2004

    • Author(s)
      Ju-Qiang Wang
    • Journal Title

      The Journal of Biological Chemistry 279・44

      Pages: 45626-45633

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inhibitory neurons from fetal rat cerebral cortex exert delayed axon formation and active migration in vitro.2003

    • Author(s)
      Kensuke Hayashi
    • Journal Title

      Journal of Cell Science 116

      Pages: 4419-4428

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inhibitory neurons from fetal rat cerebral cortex exert delayed axon formation and active migration in vitro.2003

    • Author(s)
      Hayashi K, Kawai-Hirai R, Harada A, Takata K.
    • Journal Title

      J.Cell Sci. 116(21)

      Pages: 4419-4428

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] MAP2 is required for dendrite elongation, PKA anchoring in dendrites, and proper PKA signal transduction.2002

    • Author(s)
      Akihiro Harada
    • Journal Title

      The Journal of Cell Biology 158

      Pages: 541-549

    • NAID

      120004898415

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reversal of neuronal polarity characterized by conversion of dendrites into axons in neonatal rat cortical neurons in vitro.2002

    • Author(s)
      Kensuke Hayashi
    • Journal Title

      Neuroscience 110

      Pages: 7-17

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] MAP2 is required for dendritic elongation, PKA anchoring in dendrites, and proper PKA signal transduction.2002

    • Author(s)
      Harada A, Teng J, Takei Y, Oguchi K, Hirokawa N.
    • Journal Title

      J.Cell Biol. 158(3)

      Pages: 541-549

    • NAID

      120004898415

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reversal of neuronal polarity characterized by conversion of dendrites into axons in neonatal rat cortical neurons in vitro.2002

    • Author(s)
      Hayashi K, Kawai-Hirai R, Ishikawa K, Takata K.
    • Journal Title

      Neuroscience 110(1)

      Pages: 7-17

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kensuke Hayashi: "Inhibitory neurons from fetal rat cerebral cortex exert delayed axon formation and active migration in vitro."Journal of Cell Science. 116・21. 4419-4428 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takashi Sato: "Localization of cAMP-dependent protein kinase in the actin and microtubule cytoskeletons in mouse hippocampal neurons"Neuroscience Letters. 325・2. 83-86 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akihiro Harada: "MAP2 is required for dendrite elongation, PKA anchoring in dendrites, and proper PKA signal transduction"Journal of Cell Biology. 158・3. 541-549 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kensuke Hayashi: "Reversal of neuronal polarity characterized by conversion of dendrites into axons in neonatal rat cortical neurons in vitro"Neuroscience. 110・1. 7-17 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi