| Project/Area Number |
14370004
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Chiba University |
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Principal Investigator |
MORI Chisato Chiba university, graduate school of medicine, professor, 大学院・医学研究院, 教授 (90174375)
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| Co-Investigator(Kenkyū-buntansha) |
FUKATA Hideki Chiba university, graduate school of medicine, associate professor, 大学院・医学研究院, 助教授 (00359598)
KOMIYAMA Masatoshi Chiba university, center for environment, health and field sciences, lecturer, 講師 (70175339)
KODA Masao Chiba university, graduate school of medicine, assistant professor, 大学院・医学研究院, 助手 (50361449)
TODAKA Emiko Chiba university, center for environment, health and field sciences, assistant professor, 助手 (30334212)
足達 哲也 千葉大学, 大学院・医学研究院, 助手 (60345014)
櫻井 健一 千葉大学, 大学院・医学研究院, 助教授 (80323434)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 2004: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | reproductive organ / development / DNA methylation / RLGS method / gene expression / endocrine disruptor / diethylstilbestrol / epigenetics / DNAメチレーション |
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| Research Abstract |
Neonatal C57BL/6 mice were exposed to 3 μg/pup/day of synthetic estrogen, diethylstilbestrol(DBS). At day 30, epididymidis (from male mice) and uteri (from female mice) were teken, and their status of DNA methylation were analyzed using restriction landmark genomic scanning method (RLGS). In epididymis and uterus, the methylation of 7 and 11 loci (out of about 1000 loci) were changed, respectively. Then lower dose of DBS (0.3、0.03、0.003 μg/pup/day) were examined, and clear dose dependency of the methylation change was observed.
We analyzed the loci those methylation were changed by DBS. Four loci from epididymis and 3 loci from uterus were cloned, and the characteristics of them such as GC content, chromosomal distribution, neighbor genes were examined. Four loci from epididymis were near from zinc finger protein, RNA helicase, protein kinase associated gene, retinal disease associated gene, respectively. Three loci from uterus were not close to known genes.
The mechanism by which methylation was changed, and the effect of that change on development of male reproductive organs remained to be elucidated.
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