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Association of mammalian PcG multimeric protein complexes to Hoxb8 gene

Research Project

Project/Area Number 14370041
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionChiba University

Principal Investigator

KOSEKI Haruhiko  Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (40225446)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 2003: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 2002: ¥7,200,000 (Direct Cost: ¥7,200,000)
KeywordsMouse / Polycomb / Rnf2 / Ring1 / Mph2 / Edr1 / Hox / Histon H3 / Edr1 / Topors / p53 / Mph2 / DNAダメージ
Research Abstract

To address molecular mechanisms underlying Polycomb group (PcG) -mediated repression of Hox gene expression, we have focused on how PcG proteins, Rnf2 (Ring1B), Edr1 (Rae28/Mph1), Ring1 (Ring1A) and Cbx2 (M33), and histone H3 acetylated on lysine 9 (H3K9) bind to flanking regions of the Hoxb8 gene. In 12.5 dpc embryos, association of Rnf2 to known Hoxb8 cis-acting regulatory elements was found predominantly in anterior embryonic tissues where transcription of this Hox gene is silent, whereas the presence of Edr1 and Cbx2 was observed irrespective of Hox transcriptional states. Conversely, acetylation of H3K9 at Hoxb8 was found in caudally increasing amounts in embryos, corresponding with the distribution of transcriptional activity. These results, and the developmental profile of Rnf2 association and H3K9 acetylation across the Hoxb cluster, suggest that inversely graded amounts of Rnf2-containing PcG complex and acetylated H3K9, may be involved in maintaining the anterior boundaries of Hox gene expression.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Fukuda K.: "Mesenchymal expression of Foxl1, a winged helix transcriptional factor, regulates generation and maintenance of gut associated lymphoid organs."Dev.Biol.. 255. 278-289 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada R.: "Cell-autonomous involvement of Mab21/1 is essential for lens placode development."Development. 130. 1759-1770 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyagishima, H.: "Dissociation of mammalian Polycomb-group proteins, Ring1B and Rae28/Ph1, from the chromatin correlates with configuration changes of the chromatin in mitotic and meiotic prophase."Histochem Cell Biol. 120. 111-119 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] d Graaff W: "Randomly inserted and targeted Hox/reporter fusions transcriptionally silenced in Polycomb mutants."Proc Natl Acad Sci U S A. 22. 13362-13367 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kaneko T: "The mouse YAF2 gene generates two distinct transcripts and is expressed in pre-and postimplantation embryos."Gene. 315. 183-192 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamamoto K: "The KDEL receptor modulates the endoplasmic reticulum stress response through mitogen-activated protein kinase signaling cascades."J Biol Chem.. 278. 34525-34532 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda K., Yoshida H., Sato T., Furumoto T., Mizutani-Koseki Y., Suzuki Y., Saito Y., Takemori T, Kimura M, Sato H., Nakayama T., Taniguchi M., Nishikawa S., Koseki H.: "Mesenchymal expression of Foxl1, a winged helix transcriptional factor, regulates generation and maintenance of gut associated lymphoid organs."Dev.Biol.. 255. 278-289 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada R., Mizutani-Koseki Y., Hasegawa T., Osumi N., Koseki H., Takahashi N.: "Cell-autonomous involvement of Mab21/1 is essential for lens placode development."Development. 130. 1759-1770 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyagishima, H., Isono, K., Fujimura, Y., Koseki, H.: "Dissociation of mammalian Polycomb-group proteins, Ring1B and Rae28/Ph1, from the chromatin correlates with configuration changes of the chromatin in mitotic and meiotic prophase."Histochem Cell Biol. 120. 111-119 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] d Graaff W, Tomotsune D, Oosterveen T, Takihara Y, Koseki H, Deschamps J.: "Randomly inserted and targeted Hox/reporter fusions transcriptionally silenced in Polycomb mutants."Proc Natl Acad Sci U S A. 22. 13362-13367 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kaneko T, Miyagishima H, Hasegawa T, Mizutani-Koseki Y, Isono K, Koseki H.: "The mouse YAF2 gene generates two distinct transcripts and is expressed in pre-and postimplantation embryos."Gene. 315. 183-192 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamamoto K, Hamada H, Shinkai H, Kohno Y, Koseki H, Aoe T.: "The KDEL receptor modulates the endoplasmic reticulum stress response through mitogen-activated protein kinase signaling cascades."J Biol Chem.. 278. 34525-34532 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda K.: "Mesenchymal expression of Foxl1, a winged helix transcriptional factor, regulates generation and maintenance of gut associated lymphoid organs."Dev.Biol.. 255. 278-289 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamada R.: "Cell-autonomous involvement of Mab21/1 is essential for lens placode development."Development. 130. 1759-1770 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyagishima, H.: "Dissociation of mammalian Polycomb-group proteins Ring1B and Rae28/Ph1 from the chromatin correlates with configuration changes of the chromatin in mitotic and meiotic prophase."Histochem Cell Biol. 120. 111-119 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] d Graaff W: "Randomly inserted and targeted Hox/reporter fusions transcriptionally silenced in Polycomb mutants."Proc Natl Acad Sci U S A. 22. 13362-13367 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kaneko T: "The mouse YAF2 gene generates two distinct transcripts and is expressed in pre-and postimplantation embryos."Gene. 315. 183-192 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamamoto K: "The KDEL receptor modulates the endoplasmic reticulum stress response through mitogen-activated protein kinase signaling cascades."J Biol Chem. 278. 34525-34532 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miki, T: "Mouse model of Prinzmetal angina by disruption of the inward rectifier Kir6.1"Nature Med. 8. 466-472 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Murakami, H: "Elbow knee synostosis (Eks) : A new mutation on mouse Chromosome 14"Mammalian Genome. 13. 341-344 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamaki, M: "The mouse Edr2 (Mph2) gene has two forms of mRNA encoding 90-and 36-kDa polyeptides"Gene. 288. 103-110 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki, M: "Involvement of the Polycomb-group gene Ring1B in the specification of the anterior-posterior axis in mice"Development. 129. 4171-4183 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akasaka, T: "MBLR, a new RING finger protein resembling mammalian Polycomb gene products, is regulated by cell cycle-dependent phosphorylation"Genes Cells.. 7. 835-850 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fukuda, K: "Mesenchymal expression of Foxl1, a winged helix transcriptional factor, regulates generation and maintenance of gut associated lymphoid organs"Dev.Biol.. (In press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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