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Molecular analyses of cell death and vacuole formation that are induced by abnormal protein accumulation

Research Project

Project/Area Number 14370057
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KAKIZUKA Akira  Kyoto Univ.Graduate. School of Biostudies, professor, 大学院・生命科学研究科, 教授 (80204329)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2003: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2002: ¥9,600,000 (Direct Cost: ¥9,600,000)
Keywordspolyglutamine / vacuole formation / neuronal cell death / AAA ATPase / VCP / amino acid modification / Lewy body / protein aggregates / ポリグルタミ / MJD / プロセシング / 空胞変性 / 小胞体 / 小胞体ストレス
Research Abstract

In neurodegenerative disorders, protein aggregates and vacuoles are almost universally found in degenerating neurons, suggesting the existence of similar molecular processes in, neuronal cell death. Indeed, 9 inherited neurodegenerative diseases, including Huntington disease have been shown to be caused by the expansion of CAG repeats encoding polyglutamines. We have identified VCP, a member of the AAA ATPase, as a key molecule in neurodegeneration. Namely, 1) VCP co-localizes with polyglutamine aggregates and other protein aggregates such as Lewy bodies in Parkinson disease; 2) Profound deficits in VCP's ATPase activity are found to severely affect ER quality control, leading to abnormal ER expansion and cell death, phenotypes frequently observed in many neurodegenerative diseases; 3) VCP has a potential to unfold misfolded proteins; 4) several amino acid in VCP are modified; 5) Modified VCP shows different ATPase activities. These lines of evidence raise the possibility that excessive accumulation of abnormal proteins may inactivate, VCP's ATPase via its (mis)modification in several neurodegenerative disorders, eventually leading to the neurodegenerations. A proper regulation of VCP function is thus proposed to lead to novel treatments that are effective in a broad spectrum of neurodegenerative diseases.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Maeda, H., et al.: "Effective treatment of advanced solid tumors by the combination of Arsenic Trioxide and L-Buthionine-Sulfoximine."Cell Death Differ.. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto, M., et al.: "Molecular clearance of ataxin-3 is regulated by a mammalian E4."EMBO J.. 23. 659-669 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi, T., Kakizuka, A.: "Molecular analyses of Machado-Joseph disease."Cytogenet.Genome Res.. 100. 261-275 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura, Y., Kakizuka, A.: "Polyglutamine diseases and molecular chaperones."IUBMB Life. 55. 337-345 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamei, Y., et al.: "PGC-1β/ERRL1 is an ERR protein ligand, whose expression induces a high-energy expenditure and"Proc.Natl.Acad.Sci.U.S.A.. 100. 12378-12383 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuno, Y., et al.: "Vacuole-creating protein in neuro-degenerative diseases."Neurosci.Lett.. 343. 77-80 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Higashiyama, H., et al.: "Identification of ter94, Drosophila VCP, as a modulator of polyglutamine-induced neurodegenerations in Drosophila."Cell Death Differ.. 9. 264-273 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakamoto, M., et al.: "Unequal crossing-over in unique PABP2 Mutations : a possible cause of oculopharyngeal muscular dystrophy."Arch.Neurol.. 59. 474-477 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishitoh, H., et al.: "ASK1 is essential for endoplasmic reticulum stress-induced neuronal cell death triggered by expanded polyglutamine repeats."Genes & Dev.. 16. 1345-1355 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura, Y., et al.: "Circumvention of chaperone requirement for aggregate formation of a short polyglutamine tract by the co-expression of a long polyglutamine tract."J.Biol.Chem.. 277. 37536-37541 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi, T., et al.: "Functional ATPase activity of p97/VCP is required for the quality control of endoplasmic reticulum in neuronally differentiated mammalian PC12 cells."J.Biol.Chem.. 277. 47358-47365 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maeda, H., et al.: "Effective treatment of advanced solid tumors by the combination of Arsenic 1Yioxide and L-Buthionine-Sulfoximine."Cell daath Differ.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto, M., et al.: "Molecular clearance of ataxin-3 is regulated by a mammalian E4."EMBO J.. 23. 659-669 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi, T., Kakizuka, A.: "Molecular analyses of Machado-Joseph disease."Cytogenet.Genome Res.. 100. 261-275 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura, Y, Kakizuka, A.: "Polyglutamine diseases and molecular chaperones."IUBMB Life. 55. 337-345 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamei, Y., et al.: "PPARg coactivator 1b/ERR ligand 1 is an ERR protein ligand, whose expression induces a high-energy expenditure and antagonizes obesity in mice."Proc.Natl.Acad.Sci.U.S.A.. 100. 12378-12383 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuno, Y., et al.: "Vacuole-creating protein in neurodegenerative diseases."Neurosci.Lett.. 343. 77-80 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Higashiyama, H., et al.: "Identification of ter94, Drosophila VCP, as a modulator of polyglutamine-induced neurodegenerations in Drosophila."Cell Death Differ.. 9. 264-273 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakamoto, M., et al.: "Unequal crossing-over, in unique PABP2 Mutations : a possible cause of oculopharyngeal muscular dystrophy."Arch.Neurol.. 59. 474-477 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishitoh, H., et al.: "ASK1 is essential for endoplasmic reticulum stress-induced neuronal cell death triggered by expanded polyglutamine repeats."Genes & Dev.. 16. 1345-1355 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura, Y., et al.: "Circumvention of chaperone requirement for aggregate formation of a short polyglutamine tract by the co-expression of a long polyglutamine tract."J.Biol.Chem.. 277. 37536-37541 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kobayashi, T., et al.: "Functional ATPase activity of p97/VCP is required for the quality control of endoplasmic reticulum in neuronally differentiated mammalian PC12 cells."J.Biol.Chem.. 277. 47358-47365 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maeda, H., et al.: "Effective treatment of advanced solid tumors by the combination of Arsenic Trioxide and L-Buthionine-Sulfoximine"Cell Death Differ.. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsumoto, M., et al.: "Molecular clearance of ataxin-3 is regulated by a mammalian E4"EMBO J.. 23. 659-669 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kobayashi, T., Kakizuka, A.: "Molecular analyses of Machado-Joseph disease"Cytogenet.Genome Res.. 100. 261-275 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kimura, Y., Kakizuka, A.: "Polyglutamine diseases and molecular chaperones"IUBMB Life. 55. 337-345 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kamei, Y., et al.: "PGC-1β/ERRL1 is an ERR protein ligand, whose expression induces a high-energy expenditure and"Proc.Natl.Acad.Sci.U.S.A.. 100. 12378-12383 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mizuno, Y., et al.: "Vacuole-creating protein in neuro-degenerative diseases"Neurosci.Lett.. 343. 77-80 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Higashiyama, H., et al.: "Identification of ter94, Drosophila VCP, as a modulator of polyglutamine-induced neurodegenerations in Drosophila"Cell Death Differ.. 9. 264-273 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakamoto, M., et al.: "Unequal crossing-over in unique PABP2 Mutations : a possible cause of oculopharyngeal muscular dystrophy"Archives Neurology. 59. 474-477 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishitoh, H., et al.: "ASK1 is essential for endoplasmic reticulum stress-induced Neuronal cell death triggered by expanded polyglutamine repeats"Genes & Dev.. 16. 1345-1355 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kimura, Y., et al.: "Circumvention of chaperone requirement for aggregate formation of a short polyglutamine tract by the co-expression of a long polyglutamine tract"J.Biol.Chem.. 277. 37536-37541 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kobayashi, T., et al.: "Functional ATPase activity of p97/VCP is required for the quality control of endoplasmic reticulum in neuronally differentiated mammalian PC12 cells"J.Biol.Chem.. 277. 47358-47365 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mizuno, Y., et al.: "Vacuole-creating protein in neurodegenerative diseases"Neurosci.Lett.. (in press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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