• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study for molecular mechanism of human lung adenocarcinoma.

Research Project

Project/Area Number 14370065
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionUniversity of Tsukuba

Principal Investigator

NOGUCHI Masayuki  University of Tsukuba, Graduate school of Comprehensive Human Sciences, Professor, 大学院・人間総合科学研究科, 教授 (00198582)

Co-Investigator(Kenkyū-buntansha) MORISHITA Yukio  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Associate professor, 大学院・人間総合科学研究科, 助教授 (20271562)
IIJIMA Tatsuo  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Lecturer, 大学院・人間総合科学研究科, 講師 (40222799)
OCHIAI Atsushi  National Cancer Center, Research Institute, East, Pathology Division, Chief, 支所・臨床腫瘍病理部, 部長 (60183034)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥5,600,000 (Direct Cost: ¥5,600,000)
Keywordslung adenocarcinoma / expression profile / in situ carcinoma / early advanced carcinoma / マイクロセクション法 / マイクロダイセクション法
Research Abstract

For the three years, we mainly performed the following three research projects to make clear the molecular mechanisms of the pathogenesis and the malignant progression of the lung adenocarcinoma.
1.The expression profile of adenoma induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in A/J mouse was compared with that of normal lung tissue by suppression subtractive hybridization (SSH). The mRNAs of surfactant-associated protein A (SP-A) and lysozyme showed characteristically higher transcription in the adenoma tissue than normal lung.
2.We established an immortalized human atypical adenomatous hyperplasia (AAH) cell line (PL16T) and a human non-neoplastic bronchial epithelial cell line (PL16B) from the same patient. The expression profile of PL16T was compared with that of PL16B by SSH method. The characteristically high transcription of tumor-associated calcium signal transducer 2 (TACSTD2) and S100 calcium binding protein A2 (S100A2) was detected in PL16T. Our findings indicate that TACSTD2 and S100A2 are selectively and highly expressed in AAH, which is preinvasive stage of lung adenocaricnoma, and the high expression is preserved until the lesion progresses to bronchioloalveolar carcinoma (BAC), which is in situ stage of human lung adenocarcinoma and more advanced stages.
3.Using the resected material of human lung adenocarcinoma case, the expression profile of advanced area in Noguchi type C adenocarcinoma (early but advanced bronchioloalveolar carcinoma) was compared with that of in situ lesion of the same case by SSH method. The significantly high transcription of Bax inhibitor-1 (BI-1), TACSTDI, mitochondrial cytochrome c oxidase II, and FJL12770 was detected in adnvanced lesion. The BI-1 gene expression in tumor specimens was significantly higher in BAC and carcinoma with bronchioloalveolar (BA) spreading than carcinomas without BA component. The BI-1 gene expression was restricted to the tumor cells with lepidic growth.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (40 results)

All 2005 2004 2003 2002 Other

All Journal Article (25 results) Book (2 results) Publications (13 results)

  • [Journal Article] Phenotypic characteristics of mouse lung adenoma induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone.2005

    • Author(s)
      Okubo C
    • Journal Title

      Mol Carcinogen 42

      Pages: 121-126

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Reproducibility of the diagnosis of small adenocarcinoma of the lung and usefulness of an educational program for the diagnostic criteria.2005

    • Author(s)
      Noguchi M
    • Journal Title

      Pathol Int 55

      Pages: 8-13

    • NAID

      10015458709

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Loss of function of p16 gene and prognosis of pulmonary adenocarcinoma.2005

    • Author(s)
      Tanaka R
    • Journal Title

      Cancer 103・3

      Pages: 608-615

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Diffuse pulmonary hamartoma : a case report.2005

    • Author(s)
      Minami Y
    • Journal Title

      Pathol Res Prac 200

      Pages: 813-816

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Loss of function of p16 gene and prognosis of pulmonary adenocarcinoma.2005

    • Author(s)
      Tanaka R
    • Journal Title

      Cancer 103(3)

      Pages: 608-615

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cytologic characteristic of pulmonary papillary adenoma : A case report.2004

    • Author(s)
      Minami Y
    • Journal Title

      Acta Cytologica 48・2

      Pages: 243-248

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] MMP-2 activation and stepwise progression of pulmonary adenocarcinoma : analysis of MMP-2 and MMP-9 with gelatin zymography.2004

    • Author(s)
      Iijima T
    • Journal Title

      Pathol Int 54

      Pages: 295-301

    • NAID

      10016542717

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Phenotypic differences of proliferating fibroblasts in the stroma of lung adenocarcinoma and normal bronchus tissue.2004

    • Author(s)
      Nakamura N
    • Journal Title

      Cancer Sci 95・3

      Pages: 226-232

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Anthracotic index and DNA methylation status of sputum contents can be used for identifying the population at risk of lung cancer.2004

    • Author(s)
      Konno S
    • Journal Title

      Cancer (Cancer Cytopathol) 102・6

      Pages: 348-354

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cytologic characteristic of pulmonary papillary adenoma : A case Report.2004

    • Author(s)
      Minami Y
    • Journal Title

      Acta Cytologica 48(2)

      Pages: 243-248

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phenotypic differences of proliferating fibroblasts in the stroma of lung adenocarcinoma and normal bronchus tissue.2004

    • Author(s)
      Nakamura N
    • Journal Title

      Cancer Sci 95(3)

      Pages: 226-232

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Anthracotic index and DNA methylation status of sputum Contents can be used for identifying the population at risk of lung cancer.2004

    • Author(s)
      Konno S
    • Journal Title

      Cancer (Cancer Cytopathol) 102(6)

      Pages: 348-354

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Anthracotic index and DNA methylation status of sputum contents can be used for identifying the population at risk of lung cancer.2004

    • Author(s)
      Konno S
    • Journal Title

      Cancer(Cancer Cytopathol) 102・6

      Pages: 348-354

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Pulmonary adenocarcinoma with massive lymphocyte infiltration : report of three cases.2003

    • Author(s)
      Minami Y
    • Journal Title

      Lung Cancer 42

      Pages: 63-68

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] The implication of background anthracosis in the development and progression of pulmonary adenocarcinoma.2003

    • Author(s)
      Wang D
    • Journal Title

      Cancer Sci 94・8

      Pages: 707-711

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Selection of higher molecular weight genomic DNA for molecular diagnosis from formalin-fixed material.2003

    • Author(s)
      Inadome Y
    • Journal Title

      Diagn Mol Pathol 12・4

      Pages: 231-236

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] The implication of background anthracosis in the development and progression of pulmonary adenocarcinoma.2003

    • Author(s)
      Wang D
    • Journal Title

      Cancer Sci 94(8)

      Pages: 707-711

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Selection of higher molecular weight genomic DNA for Molecular diagnosis from formalin-fixed material.2003

    • Author(s)
      Inadome Y
    • Journal Title

      Diagn Mol Pathol 12(4)

      Pages: 231-236

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Intrabronchial orthotopic propagation of human lung Adenocarcinoma : Characterizations of tumorigenicity, invasion and metastasis.2002

    • Author(s)
      Mase K
    • Journal Title

      Lung Cancer 36・3

      Pages: 271-276

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Intrabronchial orthotopic propagation of human lung Adenocarcinoma : Characterizations of tumorigenicity, invasion and metastasis.2002

    • Author(s)
      Mase K
    • Journal Title

      Lung Cancer 36(3)

      Pages: 271-276

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prognostication of small-sized primary pulmonary adenocarcinomas by histopathological and karyometric analysis.

    • Author(s)
      Minami Y
    • Journal Title

      Lung Cancer (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prognostication of small-sized primary pulmonary Adenocarcinomas by histopathological and karyometric analysis.

    • Author(s)
      Minami Y
    • Journal Title

      Lung Cancer (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chapter 26 Pulmonary Neoplasm. Diagnostic Surgical Pathology. 4th Edition

    • Author(s)
      Shimosato Y
    • Journal Title

      Lippincott Williams & Wilkins 50

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prognostication of small-sized primary pulmonary adenocarcinomas by histopathological and karyometric analysis.

    • Author(s)
      Minami Y
    • Journal Title

      Lung Cancer in press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Diffuse pulmonary hamartoma : a case report.

    • Author(s)
      Iinami Y
    • Journal Title

      Pathol Res Prac in press

    • Related Report
      2004 Annual Research Report
  • [Book] Chapter 26 Pulmonary Neoplasm. Diagnostic Surgical Pathology. 4th Edition2004

    • Author(s)
      Shimosato Y
    • Total Pages
      50
    • Publisher
      Lippincott Williams & Wilkins
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Chapter 26 Pulmonary Neoplasm.Diagnostic Surgical Pathology.4th Edition2004

    • Author(s)
      Shimosato Y
    • Total Pages
      50
    • Publisher
      Lippincott Williams & Wilkins
    • Related Report
      2004 Annual Research Report
  • [Publications] Mase K: "Intrabronchial orthotopic propagation of human lung adenocarcinoma.-Characterizations of tumorigenicity invasion and metastasis-"Lung Cancer. 36・3. 271-276 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Wang D: "The implication of background anthracosis in the development and progression of pulmonary adenocarcinoma"Cancer Sci. 94・8. 707-711 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Minami Y: "Pulmonary adenocarcinoma with massive lymphocyte infiltration : report of three cases"Lung Cancer. 42. 63-68 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Iijima T: "MMP-2 activation and stepwise progression of pulmonary adenocarcinoma.-Analysis of MMP-2 and MMP-9 with gelatin zymography-"Pathol Int. 54. 295-301 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakamura N: "Phenotypic differences of proliferating fibroblasts in the stroma of lung adenocarcinoma and normal bronchus tissue"Cancer Sci. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Minami Y: "Cytologic characteristic of pulmonary papillary adenoma : A case report"Acta Cytologica. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Shimosato Y: "Chapter 26 Pulmonary Neoplasm. Diagnostic Surgical Pathology. 4^<th> Edition"Lippincott Williams & Wilkins(印刷中).

    • Related Report
      2003 Annual Research Report
  • [Publications] Nomori H: "A case of multiple adenomatous hyperplasia of the lung. Detected by computed tomography"Jpn J Clin Oncol. 31・10. 514-516 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mase K: "Intrabronchial orthotopic propagation of human lung adenocarcinoma. -Characterizations of tumorigenicity, invasion and metastasis-"Lung Cancer. 36・3. 271-276 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tominaga T: "Telomerase activity and expression or human telomerase catalytic subunit gene in esophageal tissues"J Gastroenterol. 37. 418-427 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sekine S: "Craniopharyngiomas of adamantinomatous type harbor β-catenin gene mutations"Am J Pathol. 161・6. 1997-2001 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tokiwa T: "Colony-forming capacity of porcine liver epithelial cells in culture"In Vitoro Cell.Dev.Biol Animal. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimosato Y: "Chapter 26 Pulmonary Neoplasm. Diagnostic Surgical Pathology. 4^<th> Edition"Lippincott Williams & Wilkins(印刷中).

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi