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The development of multiple SNPs typing system by flow-arry

Research Project

Project/Area Number 14370071
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionYamaguchi University

Principal Investigator

SASAKI Kohsuke  YAMAGUCHI UNIV., SCH.OF MED PATHOLOGY, Professor, 医学部, 教授 (80116722)

Co-Investigator(Kenkyū-buntansha) OGA Atunori  YAMAGUCHI UNIV., SCH.OF MED PATHOLOGY, RESEARCH ASSOCIATE, 医学部, 助手 (90243633)
KAWAUCHI Shigeto  YAMAGUCHI UNIV., SCH.OF MED PATHOLOGY, ASSISTANT PROFESSOR, 医学部, 講師 (80284511)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2003: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2002: ¥7,200,000 (Direct Cost: ¥7,200,000)
KeywordsDNA / SNP / Flow cytometry (FCM) / Hybridization / fluorescence / high-throughput analysis / drug response / genotype / フローサイトメトリー / ゲノム / フローアレイ / 蛍光 / SNPs
Research Abstract

DNA sequence variation is associated with a certain disease and differential drug response. Single nucleotide polymorphisms (SNPs) are common type of variant. In this project, we have developed a new method for high-throughput genotyping of SNPs with microspheres of various sizes and a flow cytometer equipped with only a single laser
This system uses microspheres of various sizes with oligonucleotide probes attached by covalent bonds. Polymerase chain reaction (PCR) products labeled with Alexa 488 are hybridized to the probes and then hybridization signals are analyzed by flow cytometry. In order to type a specific SNP, two kinds of beads are bound to paired probes ; one complementary to the wild-type sequence and the other to the variant sequence. The paired beads are placed in the same tube and the number of normal beads and variant beads ratio are three to one. When the beads with higher fluorescence intensity are about three times as many as lower ones, this sample is determined wild-type genotype. When multiplex analysis is carried out, the beads for each SNP site is identified by their diameters. In this project, we have developed a novel method in which SNP genotyping with microspheres of various sizes and flowcytometer equipped with single laser. The method increases parameters in beads analysis

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Furuya T, Ikemoto K, Kawauchi S, Oga A, Tsunoda S, Hirano T, Sasaki K.: "A novel technology allowing immunohistochemical staining of a tissue section with 50 different antibodies in a single experiment."J Histochem Cytochem. 52. 205-210 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sasaki K.: "Molecular cytogenetic analysis of carcinomas of the digestive organs and its clinical application"Internal Medicine. 42. 292-294 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sasaki K, Kawauchi S: "Molecular cytogenetic analysis of solid tumors"J Orthop Sci. 8. 457-459 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Furuya T, Takita M, Tsunoda S, Kawauchi S, Hirano T, Oga A, Sasaki K: "Cell array coupled with laser scanning cytometry allows easy analysis of changes in cyclin expression during the cell cycle An application of cell array system."Method Cell Sci. 24. 41-47 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawasaki K, Suehiro Y, Umayahara K, Morioka H, Ito T, Saito T, Tsukamoto N, Sugino N, Kato H, Sasaki K.: "Cell array coupled with laser scanning cytometry allows easy analysis of changes in cyclin expression during the cell cycle An application of cell array system."Int J Mol Med. 12. 727-731 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Arai H, Ueno T, Tangoku A, Shigefumi Yoshino S, Abe T, Kawauchi S, Oga A, Furuya T, Oka M, Sasaki K.: "Cell array coupled with laser scanning cytometry allows easy analysis of changes in cyclin expression during the cell cycle An application of cell array system."Cancer Genet Cytogenet. 146. 16-21 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueno T, Tangoku A, Yoshino S, Abe T, Hayashi H, Toshimitsu H, Hashimoto K, Satoh T, Oga A, Furuya T, Oka M, Sasaki K.: "Detection of amplified oncogenes by genome DNA microarrays in human primary esophageal squamous cell carcinoma : comparison with conventional comparative genomic hybridization analysis"Clin Cancer Rese. 146. 5137-5141 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takita M, Furuya T, Sugita T, Kawauchi S, Oga A, Hirano T, Tsunoda S, Sasaki K.: "An analysis of changes in the expression of cyclins A and B1 by the cell array system during the cell cycle : Comparison between cell synchronization methods"Cytometry. 55A. 24-29 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishizaki T, Sasaki K.: "Molecular cytogenetic analysis and its clinical importance in cases of glioma"Recent Res Devel Human Pathol. 55A. 201-215 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Harada T, Okita K, Shiraishi K, Kusano N, Furuya T, Oga A, Kawauchi S, Kondoh S, Sasaki K.: "Detection of genetic alterations in pancreatic cancers by comparative genomic hybridization coupled with tissue microdissection and degenerate oligonucleotide primed polymerase chain reaction."Oncology. 62. 251-258 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Umayahara K, Numa F, Suehiro Y, Sakata A, Nawata S, Ogata H, Suminami Y, Sakamoto M, Sasaki K, Kato H.: "Comparative geomic hibridization detects genetic alterations during early stages of cervical cancer progression."Genes Chromosomes Cancer. 33. 98-102 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueno T, Tangoku A, Yoshino S, Abe T, Toshimitsu H, Furuya T, Kawauchi S, Oga A, Oka M, Sasaki K.: "Gain of 5p15 detected by comparative genomic hybridization as an independent marker of poor prognosis in patients with esophageal squamous cell carcinoma."Clin Cancer Res. 8. 526-533 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Furuya T, Ikemoto K, Kawauchi S, Oga A, Tsunoda S, Hirano T, Sasaki K.: "A novel technology allowing immunohistochemical staining of a tissue section with 50 different antibodies in a single experiment"J Histochem Cytochem.. 52(2). 205-210 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sasaki K: "Molecular cytogenetic analysis of carcinomas of the digestive organs and its clinical application"Internal Medicine. 42(3). 292-294 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sasaki K, Kawauchi S.Molecular: "cytogenetic analysis of solid tumors"J Orthop Sci. 8. 457-459 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Furuya T, Takita M, Tsunoda S, Kawauchi S, Hirano T, Oga A, Sasaki K.: "Cell array coupled with laser scanning cytometry allows easy analysis of changes in cyclic expression during the cell cycle An application of cell array system"Methods Cell Sci.. 24(1-3). 41-47 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takita M, Furuya T, Sugita T, Kawauchi S, Oga A, Hiran o T, T sun o da S, Sasaki K.: "An analysis of changes in the expression of cyclins A and B1 by the cell array system during the cell cycle : Comparison between cell synchronization methods"Cytometry. 55A(1). 24-29 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawasaki K, Suehiro Y, Umayahara K, Morioka H, Ito T, Saito T, Tsukamoto N, Sugino N, Kato H, Sasaki K.: "11q23-24 loss is associated with chromosoma instability in endometrial cancer"Int J Mol Med.. 12(5). 727-731 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Arai H, Ueno T, Tangoku A, Shigefumi Yoshino S, Abe T, Kawauchi S, Oga A, Furuya T, Oka M, Sasaki K.: "Detection of amplified oncogenes by genome DNA microarrays in human primary esophageal squamous cell carcinoma : comparison with conventional comparative genomic hybridization analysis"Cancer Genetics and Cytogenetics. 146 (1). 16-21 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueno T, Tangoku A, Yoshino S, Abe T, Hayashi H, Toshimitsu H ; Hashimoto K, Satoh T, Oga A, Furuya T, Oka M, Sasaki K.: "Prediction of Nodal Metastasis by Comparative Genomic Hybridization in Biopsy Specimens from Patients with Superficial Esophageal Squamous Cell Carcinoma"Clin Cancer Res. 9. 5137-5141 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishizaki T, Sasaki K.: "Molecular cytogenetic analysis and its clinical importance in cases of glioma"Recent Res Devel Human Pathol. 1. 201-215 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Harada T, Okita K, Shiraishi K, Kusano N, Furuya T, Oga A, Kawauchi S, Kondoh S, Sasaki K.: "Detection of genetic alterations in pancreatic cancers by comparative genomic hybridization coupled with tissue microdissection and degenerate oligonucleotide primed polymerase chain reaction"Oncology.. 62(3). 251-258 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Umayahara K, Num a F, Suehiro Y, Sakata A, Nawat S, Ogata H, Sum i n am i Sakamoto M, Sasaki K, Kato H.: "Comparative geomic hibridization detect : genetic alterations during early stages of cervical cancer progression"Genes Chromosomes Cancer. 33. 98-102 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueno T, Tangoku A, Yoshino S, Abe T, Toshimitsu H, Furuya T, Kawauchi Oga A, Oka M, Sasaki K.: "Gain of 5p 15 detected by comparative genomic hybridization as an independent marker of poor prognosis in patients with esophageal squamous cell carcinoma"Clinical Cancer Research. 8(2). 526-533 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikemoto K.: "Multicolor FISH and cytometric analyses allow classification of urothelial carcinomas into two subtypes, low- and high-grade tumors"Int J Oncol.. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Furuya T.: "A novel technology allowing immunohistochemical staining of a tissue section with 50 different antibodies in a single experiment"J Histochem Cytochem. 52・2. 205-210 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueno T.: "Prediction of nodal metastasis by comparative genomic hybridization in biopsy specimens from patients with superficial esophageal squamous cell carcinoma"Clin Cancer Res.. 9・14. 5137-5141 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Arai H.: "Detection of amplified oncogenes by genome DNA microarrays in human primary esophageal squamous cell carcinoma : comparison with conventional comparative genomic hybridization analysis"Cancer Genet Cytogenet.. 146・1. 16-21 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takita M.: "An analysis of changes in the expression of cyclins A and B1 by the cell array system during the cell cycle : comparison between cell synchronization methods"Cytometory. 55A・1. 24-29 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawasaki K.: "11q23-24 loss is associated with chromosomal instability in endometrial cancer"Int J Mol Med.. 12・5. 727-731 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Harada T.: "Detection of genetic alterations in pancreatic cancers by comparative genomic hybridization coupled with tissue microdissection and degenerate oligonucleotide primed polymerase chain reaction"Oncology. 62(3). 251-258 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Umayahara K.: "Comparative geomic hibridization detects genetic alterations during early stages of cervical cancer progression"Genes, Chromosomes & Cancer. 33. 98-102 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ueno T.: "Gain of 5p15 detected by comparative genomic hybridization as an independent marker of poor prognosis in patients with esophageal squamous cell carcinoma"Clinical Cancer Research. 8(2). 526-533 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harada T.: "Interglandular cytogenetic heterogeneity detected by comparative genomic hybridization in pancreatic cancer"Cancer Research. 62(3). 835-839 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kusano N.: "Chromosomal inbalances detected by comparative genomic hybridization are associated with outcome of patients with hepatocellular carcinoma"Cancer. 94(3). 746-751 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Izumi H.: "High telomerase activity correlates with the stabilities of genome and DNA ploidy in renal cell carcinoma"Neoplasia. 4(2). 103-111 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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