• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular pathophysiology of the biological barrier regulation of molecular passage through paracellular spaces.

Research Project

Project/Area Number 14370080
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionSapporo Medical University

Principal Investigator

SAWADA Norimasa  Sapporo Medical University, School of Medicine, Professor, 医学部, 教授 (30154149)

Co-Investigator(Kenkyū-buntansha) KOJIMA Takashi  Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 助教授 (30260764)
CHIBA Hideki  Sapporo Medical University, School of Medicine, Instructor, 医学部, 講師 (00295346)
TOBIOKA Hirotoshi  Sapporo Medical University, School of Medicine, Instructor, 医学部, 講師 (90291559)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2004: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2002: ¥5,300,000 (Direct Cost: ¥5,300,000)
Keywordstight junction / enodothelial cells / hepatocyte / nuclear receptor / gap junction / occludin / claudin / MAPK / PI3K
Research Abstract

Tight junctions are intercellular junctions adjacent to the apical most lateral membrane surface. They have two functions, a barrier function and a fence function. The former regulates the passage of ions, water and macromolecules through paracellular spaces and the latter maintains cell polarity. Once tight junctions are disturbed, illnesses such as edema, jaundice, diarrhea etc. can develop. In cancer cells, tight junctions decrease as dedifferentiation progresses. In this project, we demonstrated as follows.
1. Hepatocyte nuclear factor-4a is a key transcriptional factor of tight junction formation.
2. Claudin-1 and claudin-5 have consensus sequence in their cytoplasmic domains for MAP kinase and Protein kinase A, respectively.
3. Gap junctional interecellular communication enhances tight junction function.
4. In livers under pathological conditions, functions of gap and tight junctions are mainly regulated by p38 MAP kinase signal transduction pathway.
5. Occludin may in part mediate survival signal.
6. The expression of occludin progressively decreases in parallel with increasing grade of in human endometrial carcinomas.
7. Glyceraldehyde-derived advanced glycation end-products induces VEGF expression and reduces GDNF expression in human astrocytes, suggesting the main cause of diabetic retinopathy, in terms of highly permeable capillaries in the retina.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (77 results)

All 2005 2004 2003 2002 Other

All Journal Article (58 results) Book (1 results) Publications (18 results)

  • [Journal Article] Activation of p21CIP1/WAF1 gene and inhibition of cell proliferation by hepatocyte nuclear factor-4α.2005

    • Author(s)
      Chiba H et al.
    • Journal Title

      Exp Cell Res. 302

      Pages: 11-21

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Tight junction protein MAGI-1 is up-regulated by transfection with connexin 32 in an immortalized mouse hepatic cell line : cDNA microarray analysis.2005

    • Author(s)
      Murata M et al.
    • Journal Title

      Cell Tissue Res. 319

      Pages: 341-347

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Activation of p21^<CIP1/WAF1> gene and inhibition of cell proliferation by overexpression of hepatocyte nuclear factor-4a2005

    • Author(s)
      Chiba H et al.
    • Journal Title

      Exp Cell Res 302

      Pages: 11-21

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tight junction protein MAGI-1 is up-regulated by transfection with connexin32 in an immortalized mouse hepatic cell line: cDNA microarray analysis2005

    • Author(s)
      Murata M et al.
    • Journal Title

      Cell Tissue Res 319

      Pages: 341-347

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of occludin in human rectal carcinoid tumours as a possible marker for glandular differentiation.2004

    • Author(s)
      Tokunaga Y et al.
    • Journal Title

      Histopathology. 44

      Pages: 247-250

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] ギャップ結合とタイト結合-形態学的関係から機能的関係へ-2004

    • Author(s)
      小島隆 他
    • Journal Title

      顕微鏡学会誌 39

      Pages: 110-115

    • NAID

      10013445024

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] 血液組織関門の形成と制御の分子機構2004

    • Author(s)
      千葉英樹 他
    • Journal Title

      福岡医学雑誌 95

      Pages: 139-145

    • NAID

      120002834501

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Expression and function of tight junctions in the crypt epithelium of human palatine tonsils.2004

    • Author(s)
      Go M et al.
    • Journal Title

      J Histochem Cytochem. 52

      Pages: 1627-1638

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Expression of occludin, a tight junction-associated protein, in human lung carcinomas.2004

    • Author(s)
      Tobioka H et al.
    • Journal Title

      Virchows Archiv. 445

      Pages: 472-476

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Inhibition of MAP-kinase activity moderates changes in expression and function of Cx32 but not claudin-1 during DNA synthesis in primary cultures of rat hepatocytes.2004

    • Author(s)
      Kojima T et al.
    • Journal Title

      Med Electron Microsc. 37

      Pages: 101-113

    • NAID

      10014170849

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Thr203 of claudin-1, a putative phosphorylation site for MAP kinase, is required to promote the barrier function of tight junctions.2004

    • Author(s)
      Fujibe M et al.
    • Journal Title

      Exp Cell Res. 295

      Pages: 36-47

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] IL-1β regulates expression of Cx32, occludin and claudin-2 of rat hepatocytes via distinct signal transduction pathways.2004

    • Author(s)
      Yamamoto T et al.
    • Journal Title

      Exp Cell Res. 299

      Pages: 427-441

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Thr 207 of claudin5 is involved in size-selective loosening of the endothelial barrier by cyclic AMP.2004

    • Author(s)
      Soma T et al.
    • Journal Title

      Exp Cell Res. 300

      Pages: 202-212

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Oncogenic raf-1 regulates epithelial to mesenchymal transition via distinct signal transduction pathways in an immortalized mouse hepatic cell line.2004

    • Author(s)
      Lan MD et al.
    • Journal Title

      Carcinogenesis. 25

      Pages: 2385-2395

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Occludin expression decreases with the progression of human endometrial carcinoma.2004

    • Author(s)
      Tobioka H et al.
    • Journal Title

      Human Pathol. 35

      Pages: 159-164

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Expression of occludin in human rectal carcinoid tumours as a possible marker for glandular differentiation2004

    • Author(s)
      Tokunaga Y et al.
    • Journal Title

      Histopathology 44

      Pages: 247-250

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression and function of tight junctions in the crypt epithelium of human palatine tonsils2004

    • Author(s)
      Go M et al.
    • Journal Title

      J Histochem Cytochem 52

      Pages: 1627-1638

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of occludin, a tight-junction-associated protein, in human lung carcinomas2004

    • Author(s)
      Tobioka H et al.
    • Journal Title

      Virchows Archiv 445

      Pages: 472-476

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inhibition of MAP-kinase activity moderates changes in expression and function of Cx32 but not claudin-1 during DNA synthesis in primary cultures of rat hepatocytes2004

    • Author(s)
      Kojima T et al.
    • Journal Title

      Med Electron Microsc 37

      Pages: 101-113

    • NAID

      10014170849

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Thr^<203> of claudin-1, a putative phosphorylation site for MAP kinase, is required to promote the barrier function of tight junctions2004

    • Author(s)
      Fujibe M et al.
    • Journal Title

      Exp Cell Res 295

      Pages: 36-47

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] IL-1β regulates expression of Cx32, occludin, and claudin-2 of rat hepatocytes via distinct signal transduction pathways2004

    • Author(s)
      Yamamoto T et al.
    • Journal Title

      Exp Cell Res 299

      Pages: 427-441

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Thr^<207> of claudin-5 is involved in size-selective loosening of the endothelial barrier by cyclic AMP2004

    • Author(s)
      Soma T et al.
    • Journal Title

      Exp Cell Res 300

      Pages: 202-212

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oncogenic raf-1 regulates epithelial to mesenchymal transition via distinct signal transduction pathways in an immortalized mouse hepatic cell line2004

    • Author(s)
      Lan MD et al.
    • Journal Title

      Carcinogenesis 25

      Pages: 2385-2395

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Occludin expression decreases with the progression of human endometrial carcinoma2004

    • Author(s)
      Tobioka H et al.
    • Journal Title

      Hum Pathol 35

      Pages: 159-164

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Regulation of the blood-biliary barrier : interaction between gap and tight junctions in hepatocytes.2003

    • Author(s)
      Kojima T et al.
    • Journal Title

      Med Electron Microsc. 36

      Pages: 157-164

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] "すきま"の細胞生物学 細胞間接着装置タイト結合とヒト疾患2003

    • Author(s)
      澤田典均 他
    • Journal Title

      札幌医学雑誌 72

      Pages: 1-7

    • NAID

      110000453515

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hepatocyte nuclear factor (HNF)-4α triggers formation of functional tight junctions and establishment of polarized epithelial morpgology in F9 embryonal carcinoma cells.2003

    • Author(s)
      Chiba H et al.
    • Journal Title

      Exp Cell Res. 286

      Pages: 288-297

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cyclic AMP induces phosphorylation of claudin-5-immunoprecipitates and expression of claudin-5 gene in blood-brain-barrier endothelial cells via protem kinase A-dependent and -independent pathways.2003

    • Author(s)
      Ishizaki M et al.
    • Journal Title

      Exp Cell Res. 290

      Pages: 275-288

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Role of the p38 MAP-kinase signaling pathway for cx32 and claudin-1 in the rat liver.2003

    • Author(s)
      Kojima T et al.
    • Journal Title

      Cell Communication and Adhesion. 10

      Pages: 1-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tight junctions and human diseases.2003

    • Author(s)
      Sawada N et al.
    • Journal Title

      Med Electron Microsc. 36

      Pages: 147-156

    • NAID

      10011995656

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Regulation of the blood-biliary barrier: interaction between gap and tight junctions in hepatocytes2003

    • Author(s)
      Kojima T et al.
    • Journal Title

      Med Electron Microsc 36

      Pages: 157-164

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hepatocyte nuclear factor (HNF)-4α triggers formation of functional tight junctions and establishment of polarized epithelial morphology in F9 embryonal carcinoma cells2003

    • Author(s)
      Chiba H et al.
    • Journal Title

      Exp Cell Res 286

      Pages: 288-297

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cyclic AMP induces phosphorylation of claudin-5 immunoprecipitates and expression of claudin-5 gene in blood-brain-barrier endothelial cells via protein kinase A-dependent and -independent pathways2003

    • Author(s)
      Ishizaki T et al.
    • Journal Title

      Exp Cell Res 290

      Pages: 275-288

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Role of the p38 MAP-kinase signaling pathway for Cx32 and claudin-1 in the rat liver2003

    • Author(s)
      Kojima T et al.
    • Journal Title

      Cell Commun Adhes 10

      Pages: 1-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tight junctions and human diseases2003

    • Author(s)
      Sawada N et al.
    • Journal Title

      Med Electron Microsc 36

      Pages: 147-156

    • NAID

      10011995656

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bile canalicular barrier function and expression of tight junctional molecules in rat hepatocytes during common bile duct ligation.2002

    • Author(s)
      Takakuwa Y et al.
    • Journal Title

      Cell Tissue Res. 307

      Pages: 181-189

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Polarised distribution of carcinoembryonic antigen is associated with a tight junction in human colorectal adenocarcinomas.2002

    • Author(s)
      Tobioka H et al.
    • Journal Title

      J Pathol. 198

      Pages: 207-212

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cx32 formation and/or cx32-mediated intercellular communication induce expression and function of tight junctions in hepatocytic cell line.2002

    • Author(s)
      Kojima T et al.
    • Journal Title

      Exp Cell Res. 276

      Pages: 40-51

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hepatocyte Nuclear Factor (HNF)-4α induces expression of endothelial Fas ligand (FasL) to prevent cancer cell transmigration : A novel defense mechanism of endothelium against cancer metastasis.2002

    • Author(s)
      Osanai M et al.
    • Journal Title

      Jpn J Cancer Res. 93

      Pages: 532-541

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Barrier function of microvessels and roles of glial cell line-derived neurotrophic factor in the rat testis.2002

    • Author(s)
      Kamimura Y et al.
    • Journal Title

      Med Electron Microsc. 35

      Pages: 139-145

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] SSI Helicobacter pylori disrupts the paracellular barrier of the gastric mucosa and lends to neutrophilic gastritis in mice.2002

    • Author(s)
      Suzuki K et al.
    • Journal Title

      Virchows.Archiv. 440

      Pages: 318-324

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bile canalicular barrier function and expression of tight junctional molecules in rat hepatocytes during common bile duct ligation2002

    • Author(s)
      Takakuwa Y et al.
    • Journal Title

      Cell Tissue Res 307

      Pages: 181-189

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Polarised distribution of carcinoembryonic antigen is associated with a tight junction in human colorectal adenocarcinomas2002

    • Author(s)
      Tobioka H et al.
    • Journal Title

      J Pathol 198

      Pages: 207-212

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cx32 formation and/or cx32-mediated intercellular communication induce expression and function of tight junctions in hepatocytic cell line2002

    • Author(s)
      Kojima T et al.
    • Journal Title

      Exp Cell Res 276

      Pages: 40-51

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hepatocyte Nuclear Factor (HNF)-4α induces expression of endothelial Fas ligand (FasL) to prevent cancer cell transmigration: A novel defense mechanism of endothelium against cancer metastasis2002

    • Author(s)
      Osanai M et al.
    • Journal Title

      Jpn J Cancer Res 93

      Pages: 532-541

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Barrier function of microvessels and roles of glial cell line-derived neurotrophic factor in the rat testis2002

    • Author(s)
      Kamimura Y et al.
    • Journal Title

      Med Electron Microsc 35

      Pages: 139-145

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] SSI Helicobacter pylorus disrupts the paracellular barrier of the gastric mucosa and lends to neutrophilic gastritis in mice2002

    • Author(s)
      Suzuki K et al.
    • Journal Title

      Virchows Archive 440

      Pages: 318-324

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] HLA-DR- and CD11c-positive dendritic cells penetrate beyond well-developed epithelial tight junctions in human nasal mucosa of allergic rhinitis.

    • Author(s)
      Takano K et al.
    • Journal Title

      J Histochem Cytochem. in press

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] p38 MAP-kinasa regulates function of gap and tight junctions during regeneration of rat hepatocytes.

    • Author(s)
      Yamamoto T et al.
    • Journal Title

      J Hepatol. in press

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Glyceraldehyde-derived advanced glycation end-products (AGEs) preferentially induce VEGF expression and reduce GDNF expression in human asytrocytes.

    • Author(s)
      Miyajima H et al.
    • Journal Title

      Biochem Biophys Res Commun. in press

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ギャップ結合の病態解析法

    • Author(s)
      小島隆 他
    • Journal Title

      細胞 印刷中

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] HLA-DR-and CD11c-positive dendritic cells penetrate beyond well-developed epithelial tight junctions in human nasal mucosa of allergic rhinitis

    • Author(s)
      Takano K et al.
    • Journal Title

      J Histochem Cytochem (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] p38 MAP-kinase regulates function of gap and tight junctions during regeneration of rat hepatocytes

    • Author(s)
      Yamamoto T et al.
    • Journal Title

      J Hepatol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Glyceraldehyde-derived advanced glycation end-products preferentially induce VEGF expression and reduce GDNF expression in human astrocytes

    • Author(s)
      Miyajima H et al.
    • Journal Title

      Biochem Biophys Res Commun (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ギャップ結合の病態解析法

    • Author(s)
      小島隆 他
    • Journal Title

      細胞 (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] p38 MAP-kinasa regulates function of gap and tight junctions during regeneration of rat hepatocytes.

    • Author(s)
      Yamamoto T et al.
    • Journal Title

      J Hepatol. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Abnormal b-catenin expression in oral cancer with no gene mutation : Correlation with expression of cyclin D1 and epidermal growth factor receptor, Ki-67 labeling index and clinicopathological features.

    • Author(s)
      Odajima T et al.
    • Journal Title

      Human Pathology. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] HLA-DR-or CD11c-positive dendritic cells penetrate beyond well-developed epithelial tight junctions in human nasal mucosa of allergic rhinitis.

    • Author(s)
      Takano K et al.
    • Journal Title

      J Histochem Cytochem. (in press)

    • Related Report
      2004 Annual Research Report
  • [Book] 病気と細胞内小器官(森道夫編著)2002

    • Author(s)
      澤田典均
    • Total Pages
      236
    • Publisher
      文光堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Sawada N et al.: "Tight junctions and human diseases."Med Electron Microsc.. 36. 147-156 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kojima T et al.: "Regulation of the blood-biliary barrier: interaction between gap and tight junctions in hepatocytes."Med Electron Microsc.. 36. 157-164 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 澤田典均 他: ""すきま"の細胞生物学細胞間接着装置タイト結合とヒト疾患"札幌医学雑誌. 72. 1-7 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Chiba H: "Hepatocyte nuclear factor (HNF)-4α triggers formation of functional tight junctions and establishment of polarized epithelial morpgology in F9 embryonal carcinoma cells."Exp Cell Res.. 286. 288-297 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ishizaki M et al.: "Cyclic AMP induces phosphorylation of claudin-5-immunoprecipitates and expression of claudin-5 gene in blood-brain-barrier endothelial cells via protein kinase A-dependent and independent pathways."Exp Cell Res.. 290. 275-288 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kojima T et al.: "Role of the p38 MAP-kinase signaling pathway for cx32 and claudin-1 in the rat liver."Cell Communication and Adhesion.. 10. 1-7 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tobioka H et al.: "Occludin expression decreases with the progression of human endometrial carcinoma."Human Pathol.. 35. 159-164 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tokunaga Y et al.: "Expression of occludin in human rectal carcinoid tumours as a possible marker for glandular differentiation."Histopathology.. 44. 247-250 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小島隆 他: "ギャップ結合とタイト結合-形態学的関係から機能的関係へ-"顕微鏡学会誌. (印刷中).

    • Related Report
      2003 Annual Research Report
  • [Publications] Kojima T et al.: "Inhibition of MAP-kinase activity moderates changes in expression and function of Cx32 but not claudin-1 during DNA synthesis in primary cultures of rat hepatocytes."Med Electron Microsc.. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujibe M et al.: "Thr203 of claudin-1, a putative phosphorylation site for MAP kinase is required to promote the barrier function of tight junctions."Exp Cell Res.. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Takakuwa Y et al.: "Bile canalicular barrieifunction and expression of tight junctional molecules in rat hepatocytes during common bile duct ligation"Cell Tissue Res.. 307・2. 181-189 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tobioka H et al.: "Polarised distribution of carcinoembryonic antigen is associated with a tight junction in human colorectal adenocarcinomas"J Pathol.. 198・2. 207-212 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kojima T et al.: "Cx32 formation and/or cx32-mediated intercellular communication induce expression and function of tight junctions inhepatocytic cell line"Exp Cell Res.. 276・1. 40-51 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Osanai M et al.: "Hepatocyte Nuclear Factor (HNF)-4 α induces expression of endothelial Fas ligand (FasL) to prevent cancer cell transmigration : A novel defense mechanism of endothelium against cancer metastasis"Jpn J Cancer Res.. 93・5. 532-541 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kamimura Y et al.: "Barrier function of microvessels and roles of glial cell line-derived neurotrophic factor in the rat testis"Med Electron Microsc.. 35・3. 139-145 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chiba H et al.: "Hepatocyte nuclear factor (HNF)-4 α triggers formation of functional tight junctions and establishment of polarized epithelial morpgology in F9 embryonal carcinoma cells"Exp Cell Res.. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] 澤田典均: "病気と細胞内小器官"森道夫編著・文光堂. 236 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi