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Studies on Molecular Biology of Prostaglandin Biosynthesis by parasitic Trypanosoma

Research Project

Project/Area Number 14370087
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionOsaka University (2003)
Osaka Bioscience Institute (2002)

Principal Investigator

INOUE Tsuyoshi (2003)  Osaka University, Graduate School of Engineering, Associate Professor, 大学院・工学研究科, 助教授 (20263204)

B・K Kubata (2002)  (財)大阪バイオサイエンス研究所, 第2研究部, 研究員 (30291032)

Co-Investigator(Kenkyū-buntansha) NAGAMUNE Kisaburo  Osaka University, Molecular Protozoology, Assistant Professor, 微生物研究所, 助手 (90314418)
EGUCHI Naomi  Osaka Bioscience Institute, Department of Molecular Behavioral Biology, Sub-Head, 分子行動生物学部門, 研究副部長 (10250086)
URADE Yoshihiro  Osaka Bioscience Institute, Department of Molecular Behavioral Biology, Head, 分子行動生物学部門, 研究部長 (10201360)
MATSUMURA Hiroyoshi  Osaka University, Graduate School of Engineering, Assistant Professor, 大学院・工学研究科, 助手 (30324809)
KANEHISA Nobuko  Osaka University, Graduate School of Engineering, Lecturer, 大学院・工学研究科, 講師 (20177538)
井上 豪  大阪大学, 大学院・工学研究所, 助教授 (20263204)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 2003: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 2002: ¥7,200,000 (Direct Cost: ¥7,200,000)
KeywordsTrypanosoma / Leishumania / African trypanosomiasis / Chagas' disease / prostaglandins / anthelminitics / flavin enzymes / X-ray crystallographic analysis / シャーガス病
Research Abstract

In this study our aims are the elucidation of the functions and the three-dimensional structures of prostaglandin (PG) synthetic enzymes in parasitic protozoa (Trypanosoma, Leishmania, and Plasmodia), and the development of anti-parasitic drugs. Trypanosoma brucei prostaglandin (PG) F_<2α> synthase (TbPGFS), an aldo-ketoreductase (AKR), catalyzes the NADPH-dependent reduction of the endoperoxide moiety of PGH_2 into PGF_<2α> whose overproduction during trypanosomiasis causes abortion in infected subjects. Here we report the crystal structure of TbPGFS in complex with NADP^+ and citrate at 2.1 Å resolution. TbPGFS adopts a parallel (αβ)_8-barrel fold lacking the protrudent loops. The core active site structure is hydrophobic to bind hydrophobic substrates and contains tyrosine, lysine, histidine and aspartate known as a catalytic tetrad in other aldo-ketoreductases. Mutagenesis identifies that Tyr 52 and Asp 47 are not concerning to the enzyme reaction, showing His 110 and Lys 77 forms the catalytic dyad to perform an acid-assisted reduction in which His 110 transfers its proton to the substrate. These findings identify a novel catalytic mechanism for the biological reduction of the endoperoxide PGH_2 by an aldo-ketoreductase. The structure should allow for rational design of specific inhibitors useful to investigate the physiological roles of TbPGFS in trypanosomes. The reduction of PGH2 has been known for some time and it has also been shown that PGF_<2α> is enzymatically synthesized by a reductive cleavage of the 9, 11-endoperoxide of PGH_2^<43>. However, how the enzyme operates the reductive cleavage of the 9, 11-endoperoxide of PGH_2 has not been elucidated. We provide here the first structural information of a PGH_2 reductase in protozoa enzymes. This structure should now allow the rational design of specific inhibitors that might help investigate the physiological role of TbPGFS in trypanosomes.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (72 results)

All Other

All Publications (72 results)

  • [Publications] Inoue, T.: "The First Determination of the Inhibitor Complex Structure of Human Hematopoietic Prostaglandin D Synthase"J.Biochemistry. 135. 279-283 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Adachi, H.: "Two-liquid hanging-drop vapor-diffusion technique of protein crystallization"Jpn.J.Appl.Phys.. 43. L79-L81 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inoue, T.: "Mechanism of metal activation of human hematopoietic prostaglandin D synthase"Nature Structural Biology. 10. 291-296 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inoue, T.: "ヒト由来プロスタグランジンD合成酵素の金属イオン効果のメカニズム"日本結晶学会誌. 45. 190-195 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inoue, T.: "The significance of the flexible loop in the azurin (Az-iso2) from the obligate methylotroph Methylomonas sp.strain J."J.Mol.Biology. 333. 117-124 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Irikura, D.: "Cloning, Expression, Crystallization, and Preliminary X-Ray Analysis of Recombinant Mouse Lipocalin-type Prostaglandin D Synthase, a Somnogen-Producing Enzyme"J.Biochem.. 133. 29-32 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kabututu Z.: "Prostaglandin production from arachidonic acid and evidence for a 9,11-endoperoxide prostaglandin H_2 reductase in Leishmania."Intl.J.Parasitol. 32. 1693-1700 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamamoto, T.: "Crystallization and preliminary X-ray analysis of TBP-interacting protein from the hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1"Acta Cryst.. D59. 372-374 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Adachi, H.: "Laser Irradiated Growth of Protein Crystal."Jpn.J.Appl.Phys.. 42. 798-800 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Adachi, H.: "New practical technique for protein crystallization with floating and stirring methods."Jpn.J.Appl.Phys.. 42. L1161-L1163 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Xieyong, Y.: "Crystallization and preliminary X-ray crystallographic studies of mavicyanin from Cucurbita pepo medullosa"Acta Cryst.. D59. 1474-1476 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] F.von Delft: "Crystallization and preliminary X-ray crystallographic studies of mavicyanin from Cucurbita pepo medullosa"Structure. 11. 985-996 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Lazarus, M.: "Immunohistochemical localization of microsomal PGE synthase-1 and cyclooxygenases in male mouse reproductive organs."Endocrinology. 143. 2410-2419 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Szallies, A.: "Trypanosoma brucei causes loss of respiration competence and clonal death in the yeast Saccharomyces cerevisiae."FEBS Letters. 25998. 1-7 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kubata BK: "A key role for old yellow enzyme in the metabolism of drugs by Trypanosoma cruzi."J Exp Med. 196. 1241-1251 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okano Y: "Crystallization and preliminary X-ray crystallographic studies of Trypanosoma brucei prostaglandin F2 synthase."J Biochem. 132. 859-861 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumura, H.: "Crystal Structure of C4 Form Maize and Quaternary Complex of E.coli Phosphoenolpyruvate Carboxylases"Structure. 10. 1721-1730 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okano, Y.: "X-ray structure of Galdieria Rubisco complexed with one sulfate ion per active site"FEBS Letters. 527. 33-36 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizohata, E.: "Crystal Structure of Activated Ribulose-1,5-bisphosphate Carboxylase/Oxygenase from Green Alga Chlamydomonas reinhardtii Complexed with 2-Carboxyarabinitol-1,5-bisphosphate globisporus"J.Mol.Biol.. 316. 679-691 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Adachi, H.: "Protein Crystal Growth with a Two-Liquid System and Stirring Solution"J.Synchrotron Rad.. 11. 121-124 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Adachi, H.: "A Semiautomatic Protein Crystallization System with Preventing Evaporation of Drops and Surface Sensor of Solution"Jpn.J.Appl.Phys.. 43. L76-L78 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Adachi, H.: "Protein Crystal Processing Using a Deep-UV Laser"Jpn.J.Appl.Phys.. 43. L73-L75 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Adachi, H.: "Pre-Stirring Promotes Nucleation of Protein Crystals"Jpn.J.Appl.Phys.. 43. L243-L246 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Adachi, H.: "New Approach to Improve X-ray Diffraction Pattern of Protein Crystal Using UV-Laser Ablative Processing"Jpn.J.Appl.Phys.. 43. L297-L299 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Adachi, H.: "Effects of Solution Stirring on Protein Crystal Growth"Jpn.J.Appl.Phys.. 43. L686-L688 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Adachi, H.: "Improving the Quality of Protein Crystals Using Stirring Crystallization"Jpn.J.Appl.Phys.. 43. L522-L525 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Okano, T.Inoue, B.K.Kubata, Z.Kabututu, Y.Urade, H.Matsumura, Y.Kai: "Crystallization and Preliminary X-Ray Crystallographic Studies of Trypanosoma brucei Prostaglandin F2 Synthase"J.Biochem.. 132. 859-861 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Inoue, D.Irikura, N.Okazaki, S.Kinugasa, H.Matsumura, N.Uodome, M.Yamamoto, T.Kumasaka, M.Miyano, Y.Kai, Y.Urade: "Mechanism of Metal Activation of Human Hematopoietic Prostaglandin D Synthase"Nature Structural Biology. 10. 291-296 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] F.von Delft, T.Inoue, A.Saldanha, C.Abell, A.G.Smith, T.L.Blundel: "The Crystal Structure of decameric E.coli Ketopantoate Hydroxymethyltransferase by MAD phasing with 160 Se-Met sites reveals the position of the magnesium and product, ketopantoate."Structure. 11. 985-996 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Inoue, Y.Kai: "Mechanism of Metal Activation of Human Hematopoietic Prostaglandin D Synthase"Nihon Kessho Gakkai-shi. 45. 190-195 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Inoue, Y.Okano, Y.Kado, K.Aritake, D.Irikura, N.Uodome, N.Okazaki, S.Kinugasa, H.Shishitani, H.Matsumura, Y.Kai, Y.Urade: "The First Determination of the Inhibitor Complex Structure of Human Hematopoietic Prostaglandin D Synthase"J.Biochem.. 135. 279-283 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Inoue, S.Suzuki, N.Nishio, K.Yamaguchi, K.Kataoka, J.Tobari, X.Yong, S.Hamanaka, H.Matsumura, Y.Kai: "The significance of the flexible loop in the azurin (Az-iso2) from the obligate methylotroph Methylomonas sp.strain J"J.Mol.Biol.. 333. 117-124 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Adachi, K.Takano, Y.Hosokawa, T.Inoue, Y.Mori, H.Matsumura, M.Yoshimura, Y.Tsunaka, M.Morikawa, S.Kanaya, H.Masuhara, Y.Kai, T.Sasaki: "Laser Irradiated Growth of Protein Crystal."Jpn.J.appl.Phys.. 42. 798-800 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Adachi, H.Matsumura, K.Takano, A.Niino, T.Inoue, M.Yoshimura, Y.Mori, T.Sasaki: "New practical technique for protein crystallization with floating and stirring methods."Jpn.J.Appl.Phys.. 42. 1161-L-1163L (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Adachi, H.Matsumura, K.Takano, A.Niino, T.Inoue, Y.Mori, T.Sasaki: "Two-liquid hanging-drop vapor-diffusion technique of protein crystallization"Jpn.J.Appl.Phys.. 43. L79-L81 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Adachi, K.Takano, H.Matsumura, M.T.Inoue, Mori, T.Sasaki: "Protein Crystal Growth with a Two-Liquid System and Stirring Solution"J.synchrotron Rad.. 11. 121-124 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Adachi, K.Takano, H.Matsumura, A.Niino, T.Ishizu, T.Inoue, Y.Mori, T.Sasaki: "A Semiautomatic Protein Crystallization System with Preventing Evaporation of Drops and Surface Sensor of Solution"Jpn.J.Appl.Phys.. 43. L76-L78 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Kitano, H.Adachi, A.Murakami, H.Matsumura, K.Takano, T.Inoue, Y.Mori, S.Owa, T.Sasaki: "Protein Crystal Processing Using a Deep-UV Laser"Jpn.J.Appl.Phys.. 43. L73-L75 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Adachi, A.Niino, H.Matsumura, K.Takano, T.Inoue, Y.Mori, T.Sasaki: "Pre-Stirring Promotes Nucleation of Protein Crystals"Jpn.J.Appl.Phys.. 43. L243-L246 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Kitano, H.Adachi, A.Murakami, H.Matsumura, K.Takano, T.Inoue, Y.Mori, S.Owa, T.Sasaki: "New Approach to Improve X-ray Diffraction Pattern of Protein Crystal Using UV-Laser Ablative Processing"Jpn.J.Appl.Phys.. 43. L297-L299 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Yaoi, H.Adachi, K.Takano, H.Matsumura, T.Inoue, Y.Mori, T.Sasaki: "Effects of Solution Stirring on Protein Crystal Growth"Jpn.J.Appl.Phys.. 43. L522-L525 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Adachi, H.Matsumura, A.Niino, K.Takano, T.Kinoshita, M.Warizaya, T.Inoue, Y.Mori, T.Sasaki: "Improving the Quality of Protein Crystals Using Stirring Crystallization"Jpn.J.Appl.Phys.. 43. L522-L525 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Xie, T.Inoue, Y.Miyamoto, H.Matsumura, K.Kataoka, K.Yamaguchi, S.Suzuki, Y.Kai: "Crystallization and preliminary X-ray crystallographic studies of mavicyanin from Cucurbita pepo medullosa"Acta Cryst.. D59. 1474-1476 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamamoto, T.Matsuda, N.Sakamoto, H.Matsumura, T.Inoue, M.Morikawa, S.Kanaya, Y.Kai: "Crystallization and preliminary X-ray analysis of TBP-interacting protein from the hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1"Acta Cryst.. D59. 372-374 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Matsumura, Y.Xie, S.Shirakata, T.Inoue, T.Yoshinaga, Y.Ueno, K.Izui, Y.Kai: "Crystal Structure of C4 Form Maize and Quaternary Complex of E.coli Phosphoenolpyruvate Carboxylases."Sstructure. 10. 1721-1730 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Okano, E.Mizohata, Y.Xie, H.Matsumura, H.Sugawara, T.Inoue, A.Yokota, Y.Kai: "X-ray structure of Galdieria Rubisco complexed with one sulfate ion per active site."FEBS LETTERS. 527. 33-36 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] E.Mizohata, H.Matsumura, Y.Okano, M.Kumei, H.Takuma, J.Onodera, K.Kato, N.Shibata, T.Inoue, A.Yokota, Y.Kai: "Crystal structure of activated ribulose-l,5-bisphosphate carboxylase/oxygenase from green alga Chlamydomonas reinhardtii complexed with 2-carboxyarabinitol-l,5-bisphosphate."J.Mol.Biol.. 316. 677-689 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inoue, T.: "The First Determination of the Inhibitor Complex Structure of Human Hematopoietic Prostaglandin D Synthase"J.Biochem.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Adachi, H.: "Two-liquid hanging-drop vapor-diffusion technique of protein crystallization"Jpn.J.Appl.Phys.. 43. L79-L81 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Inoue, T.: "Mechanism of metal activation of human hematopoietic prostaglandin D synthase"Nature Structural Biology. 10. 291-296 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Inoue, T.: "ヒト由来プロスタグランジンD合成酵素の金属イオン効果のメカニズム"日本結晶学会誌. 45. 190-195 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Inoue, T.: "The significance of the flexible loop in the azurin (Az-iso2) from the obligate methylotroph Methylomonas sp.strain J."J.Mol.Biology. 333. 117-124 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Irikura, D.: "Cloning, Expression, Crystallization, and Preliminary X-Ray Analysis of Recombinant Mouse Lipocalin-type Prostaglandin D Synthase, a Somnogen-Producing Enzyme"J.Biochem.. 133. 29-32 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kabututu Z.: "Prostaglandin production from arachidonic acid and evidence for a 9,11-endoperoxide prostaglandin H_2 reductase in Leishmania"Intl.J.Parasitol. 32. 1693-1700 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamamoto, T.: "Crystallization and preliminary X-ray analysis of TBP-interacting protein from the hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1"Acta Cryst.. D59. 372-374 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Adachi, H.: "Laser Irradiated Growth of Protein Crystal"Jpn.J.Appl.Phys.. 42. 798-800 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Adachi, K.: "New practical technique for protein crystallization with floating and stirring methods"Jpn.J.Appl.Phys.. 42. L1161-L1163 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Xieyong, Y.: "Crystallization and preliminary X-ray crystallographic studies of mavicyanin from Cucurbita pepo medullosa"Acta Cryst.. D59. 1474-1476 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] F.von Delft: "Crystallization and preliminary X-ray crystallographic studies of mavicyanin from Cucurbita pepo medullosa"Structure. 11. 985-996 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Lazarus, M.: "Immunohistochemical localization of microsomal PGE synthase-1 and cyclooxygenases in male mouse reproductive organs"Endocrinology. 143. 2410-2419 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Szallies, A.: "Trypanosoma brucei causes loss of respiration competence and clonal death in the yeast Saccharomyces cerevisiae"FEBS Letters. 25998. 1-7 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kubata BK: "A key role for old yellow enzyme in the metabolism of drugs by Trypanosoma cruzi"J Exp Med. 196. 1241-1251 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okano Y: "Crystallization and preliminary X-ray crystallographic studies of Trypanosoma brucei prostaglandin F2 synthase"J Biochem. 132. 859-861 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsumura, H.: "Crystal Structure of C4 Form Maize and Quaternary Complex of E. coli Phosphoenolpyruvate Carboxylases"Structure. 10. 1721-1730 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okano, Y.: "X-ray structure of Galdieria Rubisco complexed with one sulfate ion per active site"FEBS Letters. 527. 33-36 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mizohata, E.: "Crystal Structure of Activated Ribulose-1,5-bisphosphate Carboxylase/Oxygenase from Green Alga Chlamydomonas reinhardtii Complexed with 2-Carboxyarabinitol-1,5-bisphosphate globisporus"J.Mol.Biol.. 316. 679-691 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Irikura, D.: "Cloning, Expression, Crystallization, and Preliminary X-Ray Analysis of Recombinant Mouse Lipocalin-type Prostaglandin D Synthase, a Somnogen-Producing Enzyme"J. Biochem.. 133. 29-32 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kabututu Z.: "Prostaglandin production from arachidonic acid and evidence for a 9,11-endoperoxide prostaglandin H 2 reductase in Leishmania"Intl. J. Parasitol. 32. 1693-1700 (2003)

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      2002 Annual Research Report
  • [Publications] Lazarus, M.: "Immunohistochemical localization of microsomal PGE synthase-1 and cyclooxygenases in male mouse reproductive organs"Endocrinology. 143. 2410-2419 (2002)

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      2002 Annual Research Report
  • [Publications] Szallies, A.: "Trypanosoma brucei causes loss of respiration competence and clonal death in the yeast Saccharomyces cerevisiae"FEBS Letters. 25998. 1-7 (2002)

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      2002 Annual Research Report
  • [Publications] Kubata BK: "A key role for old yellow enzyme in the metabolism of drugs by Trypanosoma cruzi"J Exp Med. 196. 1241-1251 (2002)

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      2002 Annual Research Report
  • [Publications] Okano, Y.: "Crystallization and preliminary X-ray crystallographic studies of Trypanosoma brucei prostaglandin F2 synthase"J Biochem. 132. 859-861 (2002)

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      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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