• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Construction of a transgenic rat model, which is highly sensitive to HTLV-1 infection

Research Project

Project/Area Number 14370098
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

SHIDA Hisatoshi  Hokkaido.Univ., Institute for Genetic Medicine, Professor, 遺伝子病制御研究所, 教授 (00144395)

Co-Investigator(Kenkyū-buntansha) KIDOKORO Minoru  Institute for Infectious Disease, Department of Virology III, Principal Investigator, 主任研究官 (00370958)
HAKATA Yoshiyuki  Hokkaido Univ., Institute for Genetic Medicine, Assistant professor, 遺伝子病制御研究所, 助手 (30344500)
YASUDA Jiro  Nat.Res.Inst.of Police Sci.JAPAN, Department of First Forensic Science, Chief, 室長 (10282518)
山田 雅巳  北海道大学, 遺伝子病制御研究所, 助手 (10322851)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2003: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2002: ¥8,600,000 (Direct Cost: ¥8,600,000)
KeywordsHTLV-1 / Rex / CRM1 / rat / Infection animal model
Research Abstract

Human T cell leukemia virus type1 (HTLV-1) replicates poorly in rats owing to a low activity of Rex in rat cells. Rat autholog of human CRM 1, a cofactor of Rex, is found to be responsible for the poor activity of Rex, because it does not support the Rex multimerization, which is essential for viral RNA export. In this Project, we intended to demonstrate that rat CRM1 is a major block against HTLV-1replication and expression of human CRM1 in rat cells is sufficient to recover viral protein synthesis. Finally we aimed to produce a transgenic rat expressing human CRM1. Then we have gotten the following results.
1, The human CRM1expressing rat cell lines were successfully established.
2, In the parental rat cells, Rex activity was at undetectable level, while in the human CRM1-transduced rat cells, the Rex activity was significantly enhanced to the level similar to the human cells.
3, Furthermore, the expression of human CRMI efficiently recovered the ability of HTLV-1 to produce viron proteins.
4, We could successfully produce a transgenic rat expressing human CRM1.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Hakata Y.: "The Carboxy-terminal Region of the Human Immunodeficiency Virus-1 Protein Rev has Multiple Roles in Mediating CRM1-related Rev Functions"J.Virol.. 76・16. 8079-8089 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kidokoro M.: "Large scale preparation of biologically active measles virus haemagglutinin expressed by attenuated vaccinia virus vectors."Microbes and Infection. 4・10. 1035-1044 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda J.: "Functional involvement of a novel Nedd4-like ubiquitin ligase."EMBO Rep. 3・7. 636-640 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shida H.: "Multiple roles of cellular export machinery in HTLV-1 Rex functioning."Gann monograph. 50. 61-72 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda J.: "Nedd4 regulates egress of Ebola virus-like particles from host cells."J.Virol.. 77・18. 9987-9992 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hakata Y.: "A Multi-functional Domain in Human CRM1 (exportin 1) mediates RanBP3 Binding and Multimerization of Human T-Cell Leukemia Virus Type 1 Rex Protein."Mol.Cell.Biol.. 23・23. 8751-8761 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhang X.: "Celluar requirements for CRM1 import and export."J.Biochm.. 134・5. 759-764 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakurai A.: "Regulation of human T-cell leukemia virus type-1 (HTLV-1) budding by ubiquitin ligase Nedd4."Micobes and Infection. 6・2. 150-156 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hakata Y.: "The Carboxy-terminal Region of the Human Immunodeficiency Virus-1 Protein Rev has Multiple Roles in Mediating CRM1-related Rev Functions."J.Virol. 76. 8079-8089 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kidokoro M.: "Large scale preparation of biologically active measles virus haemagglutinin expressed by attenuated vaccinia virus vectors."Microbes and Infection. 4. 1035-1044 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda J.: "Functional involvement of a novel Nedd4-like ubiquitin ligase on retrovirus budding"EMBO Rep. 3. 636-640 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shida H.: "Multiple roles of cellular export machinery in HTLV-1 Rex functioning."Gann monograph. 50. 61-72 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda J.: "Nedd4 regulates egress of Ebola virus-like particles from host cells."J.Virol.. 77. 9987-9992 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hakata Y.: "A Multi-functional Domain in Human CRM1 (exportin 1) mediates RanBP3 Binding and Multimerization of Human T-Cell Leukemia Virus Type 1 Rex Protein."Mol.Cell.Biol.. 23. 8751-8761 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhang X.: "Cellular requirements for CRM1 import and export."J.Biochm.. 134. 759-764 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakurai A.: "Regulation of human T-cell leukemia virus type-1 (HTLV-1) budding by ubiquitin ligase Nedd4."Micobes and Infection. 6. 150-156 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhang.X.: "Cellular requirements for CRM1 import and export"J.Biochm.. 134・5. 759-764 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakurai.A.: "Regulation of human T-cell leukemia virus type-1 (KTLV-1)budding by ubiquitin ligase Nedd4"Microbes and Infection. 6・2. 150-156 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shida.H.: "Multiple roles of cellular export machinery in HTLV-1 Rex functioning"Gann monograph. 50. 61-72 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yasuda.J.: "Nedd4 regulates egress of Ebola virus-like particles from host cells"J.Virol.. 77・18. 9987-9992 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hakata.Y.: "A Multi-functional Domain in Human CRM1 (exportin 1) mediates RanBP3 Binding and Multimerization of Human T-Cell Leukemia Virus Type 1 Rex Protein"Mol.Cell.Biol.. 23・23. 8751-8761 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hakata, Y.: "The Carboxy-terminal Region of the Human Immunodeficiency Virus-1 Protein Rev has Multiple Roles in Mediating CRM1-related Rev Functions"J. Virol.. 76. 8079-8089 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shida, H.: "Multiple roles of cellular export machinery in HTLV-1 Rex functioning"Gann monograph. (In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kidokoro, M.: "Large scale preparation of biologically active measles virus haemagglutinin expressed by attenuated vaccinia virus vectors"Microbes and Infection. 4. 1035-1044 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yasuda, J.: "Functional involvement of a novel Nedd4-like ubiquitin ligase on retrovirus budding"EMBO Rep.. 3. 636-640 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi