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Safety assessment of chemical substances by in vitro and in vivo magnetometry

Research Project

Project/Area Number 14370127
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionKitasato University

Principal Investigator

AIZAWA Yoshiharu  Kitasato University, School of Medicine, Professor, 医学部, 教授 (10124926)

Co-Investigator(Kenkyū-buntansha) SATOH Toshihiko  Kitasato University, School of Medicine, Associate Professor, 医学部, 助教授 (10225972)
TSUNODA Masashi  Kitasato University, School of Medicine, Professor, 医学部, 助教授 (00271221)
KUDO Yuichiro  Kitasato University, School of Medicine, Research Associate, 医学部, 助手 (60348505)
KAWAKAMI Tadashi  Kitasato University, School of Medicine, Professor, 医学部, 教授 (60177649)
KOTANI Makoto  Tokyo Denki University, School of Engineering, Professor, 工学部, 教授 (60057205)
新津谷 真人  北里大学, 医学部, 講師 (10245417)
岡田 充史  北里大学, 医学部, 助手 (40327325)
渡邊 光康  北里大学, 医学部, 助手 (50327342)
Project Period (FY) 2002 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2002: ¥7,200,000 (Direct Cost: ¥7,200,000)
Keywordsmagnetometry / man-made-mineral fibers / rock wool / cyototoxicity / inhalation / biopersistence / nose-only inhalation / safety of chemical substance / クリソタイル / 肺胞マクロファージ / 細胞傷害性 / 細胞磁界測定 / 肺内繊維数 / 肺磁界測定 / 乳酸脱水素酵素 / アポトーシス / Micro glass fiber
Research Abstract

Several thousands of chemical substances are newly produced in Japan. A system of safety assessment for these new substances is required. Recently, various kinds of man-made-mineral fibers (MMMFs) have been used, since serious health disorders including mesotheliomas were reported by an exposure to asbestos. We evaluated the cytotoxicity of 4 types of MMMFs, fibrous titanium dioxide (fTiO_2), potassium octatitanate whisker (PT), microglass (MG) and rock wool (RW) by cytomagnetometry, as well as LDH release assays, morphological observations and apoptotis detections. Also evaluated were other substances such as indium arsenide (InAs), CdO and photocopier toner.
By magnetometry, the remanent fields radiated from magnetites engulfed by alveolar macrophages of rats decreased rapidly and this phenomenon is named ‘relaxation'. The relaxation is thought attributed to the random rotation of phagosomes driven by cytoskeleton. Cytotoxicity on the basis of delayed relaxation and increased LDH release was observed as follows, fTiO_2 and PT>MG>RW. The exposure to CdO caused marked effects on the relaxation and LDH release, while the effects of both InAs and photocopier toner were negative.
Male rats were exposed to target exposure concentration of RW 100 fibers/m^3 by nasal inhalation 3 hours daily for 5 consecutive days. Rats were sacrificed shortly after exposure, 1,2 and 4 weeks after exposure and the fiber counts and sizes in the lungs were analyzed. Both the length and width significantly decreased 4 weeks after exposure. The persistence of RW in the lungs estimated by a half-life was shorter than previously reported value of chrysotile fibers. The histological changes of the lungs in RW-exposed rats were minimum.
The in vitro results of RW exposure coincided with the in vivo results. To confirm the usefulness of magnetometry as a screening test, further study is necessary to examine the in vivo study using chemical substance with positive in vitro study.

Report

(5 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (24 results)

All 2005 2003 2002 Other

All Journal Article (18 results) Publications (6 results)

  • [Journal Article] Behavior of new type of rock wool (HT wool) in lungs after exposure by nasal inhalation in rats.2005

    • Author(s)
      Yuichiro Kudo
    • Journal Title

      Environmental Health and Preventive Medicine 10(5)

      Pages: 239-248

    • NAID

      110002973552

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The cytotoxicity of micrograss fibers in alveolar macrophages of Fischer 344 rets evaluated by cell magnetometry,cytochemmistry and morphology2005

    • Author(s)
      Hisako shinji
    • Journal Title

      Enviornmental Health and Preventive Medicine 10(2)

      Pages: 111-119

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Behavior of new type of rock wool (HT Wool) in lungs after exposure by nasal inhalation in rats.2005

    • Author(s)
      Yuichiro Kudo
    • Journal Title

      Environmental Health and Preventive Medicine 10(5)

      Pages: 239-248

    • NAID

      110002973552

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] The cytotoxicity of microglass fibers on alveolar macrophages of Fischer 344 rats evaluated by cell magnetometry, cytochemistry and morphology.2005

    • Author(s)
      Hisako Shinji
    • Journal Title

      Environ.Health Prev.Med. 10

      Pages: 111-119

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The cytotoxicity of micrograss fibers on alveolar macrophages of Fischer 344 rats evaluated by cell magnetometry cytochemistry and morphology2005

    • Author(s)
      Hisako Shinji
    • Journal Title

      Environmental Health and Preventive Medicine 10(2)

      Pages: 111-119

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The cytotoxicity of micrograss fibers on alveolar macrophages of Fischer 344 rats evaluated by cell magnetometry, cytochemistry and morphology2005

    • Author(s)
      Hisako Shinji
    • Journal Title

      Environmental Health and Preventive Medicine 10(2)(In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Comparative cytotoxicity study of rock wool and chrysotile by cell nmagnetometric evaluation2003

    • Author(s)
      Yuichiro Kudo
    • Journal Title

      Inhalation Toxicorogy 15

      Pages: 1275-1295

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Magnetometric evaluation of cadmium oxide-induced toxicity to pulmonary alveolar macrophages of syrian golden hamsters.2003

    • Author(s)
      Masato Niitsukya
    • Journal Title

      Journal of Toxicology and Environmental Health 66(4)

      Pages: 365-378

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparative cytotoxicity study of rock wool and chrysotile by cell magnetometric evaluation.2003

    • Author(s)
      Yuichiro Kudo
    • Journal Title

      Inhal.Toxicol 15

      Pages: 1275-1295

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Magnetometric evaluation of cadmium oxide-induced toxicity to pulmonary alveolar macrophages of syrian golden hamsters.2003

    • Author(s)
      Masato Niitsuya
    • Journal Title

      Journal of Toxicology and Environmental Health 66(4)

      Pages: 365-378

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparative cytotoxicity study of rock wool and chrysotile by cell magnetometric evaluation2003

    • Author(s)
      Yuichiro Kudo
    • Journal Title

      Inhalation Toxicorogy 15

      Pages: 1275-1295

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Magnetometric evaluation of cadmium oxide-induced toxicity to pulmonary alveolar macrophages of Syrian golden hamsters.2003

    • Author(s)
      Masato Niitsuya
    • Journal Title

      Journal of Toxicology and Environmental Health 66(4)

      Pages: 365-378

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Differences in the effects of fibrous and particulate titanium dioxide on alveolar macrophages of Fischer 344 rats2002

    • Author(s)
      Mitsuyasu Watanabe
    • Journal Title

      Journal of toxicology and Environmental Health Part A,65

      Pages: 1047-1060

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Magnetometric Evaluation for Cytotoxicity of Potassium Octatitanate Whisker on Alveolar Macrophages of Fischer 344 Rats2002

    • Author(s)
      Mitsuyasu Watanabe
    • Journal Title

      J Occup Health 44

      Pages: 321-328

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Differences in the effects of fibrous and particulate titanium dioxide on alveolar macrophages of Fischer 344 rats.2002

    • Author(s)
      Mitsuyasu Watanabe
    • Journal Title

      Journal of Toxicology and Environmental Health. Part A 65

      Pages: 1047-1060

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Magnetometric evaluation for the cytotoxicity of potassium octatitanate whisker on alveolar macrophages of Fischer 344 rats.2002

    • Author(s)
      Watanabe Mitsuyasu
    • Journal Title

      J.Occup.Health 44

      Pages: 321-328

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differences in the effects of fibrous and particulate titanium dioxide on alveolar macrophages of Fischer 344 rats2002

    • Author(s)
      Mitsuyasu Watanabe
    • Journal Title

      Journal of Toxicology and Environmental Health Part A,65

      Pages: 1047-1060

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vitro toxicity of indium arsenide to alveolar macrophages evaluated by magnetometry, cytochemistry and morphological analysis2002

    • Author(s)
      Mitsushi Okada
    • Journal Title

      Toxicology Letters 134

      Pages: 185-194

    • Related Report
      2004 Annual Research Report
  • [Publications] Mitsuyasu Watanabe: "DIFFERENCES IN THE EFFECTS OF FIBROUS AND PARTICULATE TITANIUM DIOXIDE ON ALVEOLAR MACROPHAGES OF FISCHIER 344 RATS"Journal of Toxicology and Environmental Health. Part A, 65. 1047-1060 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mitsuyasu Watanabe: "Magnetometric Evaluation for Cytotoxicity of Potassium Octatitanate Whisker on Alveolar Macrophages of Fischer 344 Rats"J Occup Health. 44. 321-328 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mitsushi Okada: "In vitro toxicity of indium arsenide to alveolar macrophages evaluated by magnetometry, cytochemistry and morphological analysis"Toxicology Letters. 134. 185-194 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yuichiro Kudo: "COMPARATIVE CYTOTOXICITY STUDY OF ROCK WOOL AND CHRYSOTILE BY CELL MAGNETOMETRIC EVALUATION"Inhalation Toxicorogy. 15. 1275-1295 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mitsuyasu Watanabe: "DIFFERENCES IN THE EFFECTS OF FIBROUS AND PARTICULATE TITANIUM DIOXIDE ON ALVEOLAR MACROPHAGES OF FISCHER 344 RATS"Journal of Toxicology and Environmental Health. Part A, 65. 1047-1060 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mitsuyasu Watanabe: "Magnetometric Evaluation for Cytotoxicity of Potassium Octatitanate Whisker on Alveolar Macrophages of Fischer 344 Rats"J Occup Health. 44. 321-328 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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