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Developtment of sensitive and rapid methods for analysis of inorganic toxic substances in human specimens

Research Project

Project/Area Number 14370153
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

SUZUKI Osamu  Hamamatsu University School of Medicine, Professor, 医学部, 教授 (70093044)

Co-Investigator(Kenkyū-buntansha) WATANABE Kanako  Hamamatsu University School of Medicine, Associate Professor, 医学部, 助教授 (70288546)
MINAKATA Kayoko  Hamamatsu University School of Medicine, Assistant Professor, 医学部, 講師 (70115509)
HATTORI Hideki  Aichi Medical University, Associate Professor, 医学部, 助教授 (30107817)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2002: ¥8,000,000 (Direct Cost: ¥8,000,000)
KeywordsInorganic compound analysis / Ion chromatography / Nitrite anion / Nitrate nion / ESR / Manganese / Molybdenum / Arsenous acid / 原子吸光分析 / アジ化物 / 鉄 / 銅
Research Abstract

1)Ion chromatographic analysis of inorganic toxic substances
On July 25, 1998, the Wakayama Curry Incident, in which 63 people were injured by arsenic and 4 killed, took place. In the same year, many copycat poisoning incidents using other inorganic toxins followed. Therefore, it was essential for us to be prepared to cope with any type of poison analysis including inorganic ones. We introduced a full system of ion chromatography with an autosuppressor, which could cover the analysis of almost every type of inorganic compounds. As the first study using the above instrument, we established a detailed procedure for analysis of nitrite and nitrate anions in human whole blood. There was a serious problem in the assays of nitrite anion in whole blood, because the retention time of nitrite was very close to that of chloride anion which is abundantly present in whole blood. Before application of a sample to ion chromatography, we succeeded in removing the chloride anion using the combination of cartridges of On Guard II AG and On Grand II H. It was found that most of nitrite was oxidized to nitrate within 3 h in whole blood. The recovery rate for nitrite from whole blood was more than 90 %. The linearity was confirmed in the range of 50-100 μg/ml of whole blood. The detection limit of nitrate was sufficientry low. These data were arranged into a scientific paper, which is now in press in an international journal.
2)ESR analysis of inorganic substances
Ions of manganese(Mn), molybdenum(Mo) and thiocyanate were tried to be measured by ESR after suitable pretreatments. Mn ion shows paramagnetic property and was thus measurable by ESR only after asking a sample. Mo ion reacted with diethyldithiocarbamate and the complex could be measured by ESR with high sensitivity after reduction by oxalic acid. Thiocyanate could be also measured after complex formation. These results were published in analytical journals.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (26 results)

All 2005 2004 2003 2002 2000 Other

All Journal Article (14 results) Book (2 results) Publications (10 results)

  • [Journal Article] Simultaneous analysis of nitrite and nitrate in whole blood by ion chromatography2005

    • Author(s)
      O.Suzuki
    • Journal Title

      J.Liq.Chromatogr.R.T. (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Simultaneous analysis of nitrite and nitrate in whole blood by ion chromatography2005

    • Author(s)
      O.Suzuki et al.
    • Journal Title

      J.Liq.Chromatogr.R.T. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Rapid determination of thiocyanate in biological fluids by use of electron spin resonance2005

    • Author(s)
      K.Minakata
    • Journal Title

      Jpn.J.Forensic Toxicol. 23・1

      Pages: 41-44

    • NAID

      120005314068

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Determination of nitrite in biological fluids by use of electron spin resonance2004

    • Author(s)
      K.Minakata
    • Journal Title

      Anal.Biochem. 325

      Pages: 168-170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Accumulation of copper in kidney of spontaneously hypertensive rats, a preliminary study2004

    • Author(s)
      K.Minakata
    • Journal Title

      BioFactors 22

      Pages: 29-31

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] determination of nitrite in biological fluids by use of electron spin resonance2004

    • Author(s)
      K.Minakata at al.
    • Journal Title

      Anal.Biochem. 325

      Pages: 168-170

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] New molybdenum compound suitable for determination by electron paramagnetic resonance2004

    • Author(s)
      K.Minakata at al.
    • Journal Title

      Anal.Chim.Acta 512

      Pages: 355-357

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Accumulation of copper in kidney of spontaneously hypertensive rats, a preliminary study2004

    • Author(s)
      K.Minakata at al.
    • Journal Title

      Bio Factors 22

      Pages: 29-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Manganese levels in tissues of paraquat dosed and/or magnesium restricted rats measured by an ESR method2004

    • Author(s)
      K.Minakata
    • Journal Title

      Jpn.J.Forensic Toxicol. 22・3

      Pages: 193-195

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Sensitive analysis of alkyl alcohols as decomposition products of alkyl nitraites in human whole bood and urine by headspace capillary GC with cryogenic oven trapping2003

    • Author(s)
      K.Watanabe-Suzuki
    • Journal Title

      J.Chromatogr.Sci. 41.1

      Pages: 63-66

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Sensitive analysis of alkyl alcohols as decomposition products of alkyl nitrites in human whole blood and urine by headspace capillary GC with cryogenic oven trapping2003

    • Author(s)
      K.Watanabe-Suzuki et al.
    • Journal Title

      J.Chromatogr.Sci. 41

      Pages: 63-66

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of paraquat on essential antioxidant elements in osteogenic disorder Shinogi rat2002

    • Author(s)
      K.Minakata
    • Journal Title

      J.Toxicol.Environ.Health, PartA 65

      Pages: 143-147

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of paraquat on essential antioxidant elements in osteogenic disorder Shionogi rat2002

    • Author(s)
      K.Minakata et al.
    • Journal Title

      J.Toxicol.Environ.Health, Part A 65

      Pages: 143-147

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] New molybdenum compound suitable for determination by electron paramagnetic resonance2000

    • Author(s)
      K.Minakata
    • Journal Title

      Anal.Chim.Acta 512

      Pages: 355-357

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Book] Drugs and Poisons in Humans. A Handbook of Practical Analysis2005

    • Author(s)
      O.Suzuki
    • Total Pages
      672
    • Publisher
      Springer Verlag
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Drugs and Poisons in Humans. A Handbook of Practical Analysis2005

    • Author(s)
      O.Suzuki et al.(eds.)
    • Publisher
      Springer Verlag Berlin
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] K.Minakata: "The restriction of all minerals in the diet enhancing paraquat toxicity is regarded primarily as the shortage of Mg"Legal Med.. 5. S110-S112 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Minakata: "Paraquat enhances nephrocalcinosis under Mg restriction in the diet"Jpn.J.Forensic Toxicol.. 21・3. 243-255 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Watanabe: "Stability of alkyl alcohols as decomposition products of alkyl nitrites in human whole blood"Jpn.J.Forensic Toxicol.. 21・3. 256-261 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Minakata: "Determination of nitrite in biological fluids by use of electron spin resonance"Anal.Biochem.. 325. 168-170 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 南方 かよ子: "電子スピン共鳴法を用いたモリブデンの定量法"Biomed.Res.Trace Elements. 15・1. 82-84 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Minakata: "Effects of praquat on essential antioxidant elements in osteogenic disorder Shionogi rat"J. Toxicol. Environ. Health, Part A. 65. 143-147 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Minakata: "Diquat markedly changes iron and copper concentrations in tissues and plasma of ODS rats"Jpn. J. Forensic Toxicol.. 20・3. 277-283 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 南方かよ子: "ビタミンC欠ラットの電子スピン共鳴法を用いたマンガンの定量"Biomedical Research on Trace Elements. 13・4. 320-321 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Watanabe-Suzuki: "Sensitive analysis of alkyl alcohols as decomposition products of alkylnitrites in human whole blood and urine by headspace capillary GC with cryogenic oven trapping"J. Chromatogr. Sci.. 41.1(in press). (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 鈴木 修: "薬毒物分析実験ハンドブック"株式会社 じほう. 604 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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