| Project/Area Number |
14370154
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Osaka University |
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Principal Investigator |
MATOBA Ryoji Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (20107056)
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| Co-Investigator(Kenkyū-buntansha) |
MAENO Yoshitaka Nagoya City University, Medical School, Associate Professor, 医学部, 助教授 (00145749)
OSAWA Motoki Yamagata University, School of Medicine, Professor, 医学部, 教授 (90213686)
NAKATOME Masato Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (90263251)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | diagnostic marker / quantitative RT-PCR / apoptosis / ischemia-reperfusion / Bcl-2 / Bax / 心筋梗塞 / 虚血誘発性アポトーシス / 培養心筋細胞 / アポトーシス関連遺伝子 |
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| Research Abstract |
The purpose of our study was to achieve reliable parameter as a diagnostic marker in an early stage of acute myocardial infarction to forensic autopsy cases. The Bax/Bcl-2 mRNA ratio was examined by quantitative RT-PCR methods using a cardiomyocyte model of hypoxia/reoxygenation. There was a increase in Bax/Bcl-2 mRNA ratio in both groups of 12-hour hypoxia and 24-hour hypoxia. Moreover, DNA fragmentation of cardiomyocytes was also detected by agarose gel electrophoresis. On the other hand, there was a decrease in Bax/Bcl-2 mRNA ratio in cardiomyocytes of reoxygenation groups which were exposed to reoxygenation for 3 hours. These results suggest that Bax/Bcl-2 mRNA ratio, which is a parameter of apoptosis, may be a reliable parameter as a diagnostic marker in an early stage of acute myocardial infarction to forensic autopsy cases. However, it would be difficult to detect apoptotic cells owing to the effects of the postmortem change.
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