| Project/Area Number |
14370191
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Saitama Medical School |
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Principal Investigator |
FUJIWARA Kenji Saitama Medical School, Department of Medicine, Professor, 医学部, 教授 (80101088)
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| Co-Investigator(Kenkyū-buntansha) |
MOCHIDA Satoshi Saitama Medical School, Department of Medicine, Professor, 医学部, 教授 (20219968)
NAGOSHI Sumiko Saitama Medical School, Department of Medicine, Associate Professor, 医学部, 助教授 (50306271)
MATSUI Atsushi Saitama Medical School, Department of Medicine, Assistant Professor, 医学部, 講師 (40260484)
INAO Mie Saitama Medical School, Department of Medicine, Assistant Professor, 医学部, 講師 (70286037)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥3,600,000 (Direct Cost: ¥3,600,000)
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| Keywords | Osteopontin / SNP / Th1 immune reaction / Cytokine / Chronic hepatitis C / Auto immune hepatitis / Interferon / Hepatitis activity / オステオポンチン / サイトカイン |
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| Research Abstract |
Th1 immune reaction is essential for eradication of HCV during interferon (IFN) therapy as well as development of hepatocyte injury in patients with chronic hepatitis C. Osteopontin, an extracellular matrix with RGD sequence, is a cytokine crucial for the initiation of Th1 immune reaction. Recently, we identified 4 SNPs (nt -155, -433, -616, -1,748) in the promoter region of osteopontin gene, and found that SNPs at nt -155, nt -616 and nt -1,748 showed linkage disequilibrium with D' and r^2 greater than 0.937 to each others. Alleles of these SNPs were determined in 185 patients with chronic hepatitis C by INVADER assay. We demonstrated that SNP at nt -433 may be a marker to reflect hepatitis activity in patients with chronic hepatitis C. Also, we found that SNPs at nt -155, nt -616 and nt -1,748 as well as SNP at nt -433 may be useful as markers to estimate efficacy of IFN therapy with high positive predictive value in chronic hepatitis C patients From these data, we concluded that SNPs in promoter region of osteopontin gene may be crucial in regulation of Th1 immune reaction against HCV.
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