| Project/Area Number |
14370233
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Osaka City University |
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Principal Investigator |
OMURA Takashi (2005) Osaka City University, Graduate School of Medicine, research associate, 大学院・医学研究科, 助手 (70295707)
吉川 純一 (2002-2004) 大阪市立大学, 大学院・医学研究科, 教授 (60275245)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIYAMA Minoru Osaka City University, Graduate School of Medicine, associate professor, 大学院・医学研究科, 助教授 (30240956)
大村 崇 大阪市立大学, 大学院・医学研究科, 助手 (70295707)
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| Project Period (FY) |
2002 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2005: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥3,600,000 (Direct Cost: ¥3,600,000)
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| Keywords | Stem cell / sono-therapy / myocardial infarction / heart failure / Myocatrdial infarction / Remodeling / Cell therapy / Stem cell / Signal transduction / Echocardiography |
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| Research Abstract |
Cell transplantation offers the promise in the restoration of ventricular function after an extensive myocardial infarction, but the optimal cell therapy remains controversial. Vascular endothelial growth factor (VEGF) plays an important role in inducing angiogenesis. Mesenchymal stem cells (MSCs) may have potential for differentiation to several types of cells, including myocytes. We showed that transplantation of VEGF-expressing MSCs could effectively treat acute myocardial infarction (MI) by providing enhanced cardioprotection, followed by angiogenic effects in salvaging ischemic myocardium. Moreover, to examine the effects of microbubble destruction with ultrasound (MB) combined with bone marrow derived mononuclear cell transplantation (BMT) into ischaemic tissues in rat hind limb ischaemia. MB treatment increased vascular endothelial growth factor mRNA as assessed by real time polymerase chain reaction. At four weeks, the MB group had increases in laser Doppler blood flow index, angiographically detectable collateral vessels, and capillary to muscle fiber ratio in ischaemic limbs compared with the vehicle treated group. Secondly, rats were allocated to vehicle treatment, BMT (5×10(6) cells/rat), or a combination of MB and BMT (MB+BMT) at seven days after hind limb ischaemia. BMT treatment significantly increased LDBFI, angiographic score, and capillary to muscle fiber ratio compared with vehicle treatment. MB may be a useful technique to enhance BMT induced neovascularisation and improve the effect of cell therapy.
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