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Therapeutic strategies for inherited neurodegerative disorders using gene transfer and stem-cell transplantation technologies

Research Project

Project/Area Number 14370253
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionNippon Medical School

Principal Investigator

SHIMADA Takashi  Nippon Medical School, Professor, 大学院・医学研究科, 教授 (20125074)

Co-Investigator(Kenkyū-buntansha) MIGITA Makoto  Nippon Medical School, Associate professor, 医学部, 助教授 (50256963)
MIYAKE Koichi  Nippon Medical School, Assistant professor, 医学部, 講師 (90267211)
MODHIZUKI Hideki  Juntendo University, Associate professor, 医学部, 講師 (90230044)
NISHI Katsunori  Tokyo Metropolitan Inst for Neuroscience, Dept Neurology, Director, 副参事研究員 (00138257)
平井 幸彦  日本医科大学, 医学部, 講師 (10089617)
鈴木 聡  日本医科大学, 医学部, 助手 (70246940)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2004: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥7,200,000 (Direct Cost: ¥7,200,000)
Keywordsgene therapy / cell therapy / lysosomal disorders / stem cells / neural stem cells / AAV vector / 異染性ロイコジストロフィー / アリルサルファターゼ / 多能性幹細胞 / 脂肪組織由来幹細胞 / HIVベクター / 異染性白質ジストロフィー / 多能性骨髄幹細胞
Research Abstract

1.Gene therapy : We examined the utility of recently identified sulfatase activating enzyme (FGE) for expression of arylsulfatase A(ASA) in gene therapy of metachromatic leukodustrophy(MLD). Our data demonstrated that FGE co-expression is essential for synthesis and secretion of functional ASA both in vitro and in vivo. In a therapeutic model experiment, we generated AAV1 vector carrying either ASA or FGE cDNA. Simultaneous injection of these vectors into the hippocampus of MLD mice significantly increased ASA activity and decreased sulfatide accumulation in the wide areas of the brain. Significant improvement of behavior was confirmed by the rotarod test and the walking pattern. These result support that FGE co-expression is essential for efficient synthesis of functional ASA and have significant implications for the development of MLD gene therapy.
2.Stem cell therapy : Using chimeric mice stably reconstituted with bone marrow cells from GFP transgenic mice, we demonstrated that mesenchymal stem cells derived from bone marrow (BSC) could differentiate various cell lineages. We also succeeded in preparing pluripotent stem cells from adipose tissues(ASC). The feasibility of cell therapy of MLD was demonstrate by direct inoculation of neural progenitor cells stably tranduced with lentiviral vector carrying ASA cDNA. Genetically engineered stem cells may be useful for therapy for neurodegenerative disorders.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (72 results)

All 2005 2004 2003 2002 Other

All Journal Article (58 results) Book (1 results) Patent(Industrial Property Rights) (1 results) Publications (12 results)

  • [Journal Article] An asymptomatic heterozygous female with Fabry disease : implications for enzyme replacement therapy.2005

    • Author(s)
      Inagaki, S., et al.
    • Journal Title

      J. Nippon Med. Sch. 72

      Pages: 387-390

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Co-expression of FGE is essential for synthesis and secretion of functional arylsulfatase A in a mouse model of metachromatic leukodystrophy.2005

    • Author(s)
      Takakusaki, Y., et al.
    • Journal Title

      Hum. Gene Ther. 16

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Role of bone marrow cells in the healing process of mouse experimental glomerulonephritis.2005

    • Author(s)
      Hayakawa, M., et al.
    • Journal Title

      Ped. Research 58

      Pages: 323-328

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An asymptomatic heterozygous female with Fabry disease : implications for enzyme replacement therapy.2005

    • Author(s)
      Inagaki, S., Migita, M., Hayakawa, M., Yoshida, J., Ishizaki, M., Kotani, M., Sakuraba, H., Shimada, T., Murakami, M., Fukunaga, Y.
    • Journal Title

      J.Nippon Med.Sch. 72

      Pages: 387-390

    • NAID

      130004146823

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Co-expression of FGE is essential for synthesis and secretion of functional arylsulfatase A in a mouse model of metachromatic leukodystrophy.2005

    • Author(s)
      Takakusaki, Y., Hisayasu, S., Hirai, Y., Shimada, T.
    • Journal Title

      Hum.Gene Ther. 16

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Role of bone marrow cells in the healing process of mouse experimental glomerulonephritis.2005

    • Author(s)
      Hayakawa, M., Ishizaki, M., Hayakawa, J., Migita, M., Nurakami, M., Shimada, T., Fukunaga, Y.
    • Journal Title

      Ped.Research 58

      Pages: 323-328

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] HGF receptor is a coreceptor for adeno-associated virus type 2 (AAV2) infection.2005

    • Author(s)
      Kashiwakura, Y., Tamayose, K., et al.
    • Journal Title

      J.Viol. 79

      Pages: 609-614

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Adipogenic defferentiation by adipose-derived stem cells harvested from GFP transgenic mice - including relationship of sex differences-.2004

    • Author(s)
      Ogawa, R., et al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 23

      Pages: 871-877

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] HGF receptor is a coreceptor for adeno-associatedvirus type 2 (AAV2) infection.2004

    • Author(s)
      Kashiwakura, Y., et al.
    • Journal Title

      J. Viol. 79

      Pages: 609-614

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chondrogenic and osteogenic differentiation of adipose-derived stem cells isolated from GFP transgenic mice.2004

    • Author(s)
      Ogawa, R., et al.
    • Journal Title

      J. Nippon Med. Sch. 71

      Pages: 240-241

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Recipient non-hematopoietic bone marrow cells in the intestinal graft after fetal small intestinal transplantation.2004

    • Author(s)
      Kato, Y., et al.
    • Journal Title

      Pediatr. Surg. Int. 20

      Pages: 1-4

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic and Chondrogenic Differentiation by Adipose-derived Stem Cells Harvested from GFP Transgenic Mice.2004

    • Author(s)
      Ogawa, R., Mizuno, H., Watanabe, A., Migita, A., Shimada, T., Hyakusoku, H.
    • Journal Title

      Biochem.Biophys.Res.Commun. 23

      Pages: 871-877

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Adipogenic defferentiation by adipose-derived stem cells harvested from GEP transgenic mice -including relationship of sex differences-.2004

    • Author(s)
      Ogawa, R., Mizuno, H., Watanabe, A., Migita, M., Hyakusoku, H., Shimada, T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 319

      Pages: 511-517

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] HGF receptor is a coreceptor for adeno-associated virus type 2 (AAV2) infection.2004

    • Author(s)
      Kashiwakura, Y., Tamayose, K., Iwabuti, K., Hirai, Y., Shimada, T., Matsumoto, K., Nakamura, T., Oshimi, K., Daiwa, H.
    • Journal Title

      J.Viol. 79

      Pages: 609-614

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chondrogenic and osteogenic differentiation of adipose-derived stem cells isolated from GFP transgenic mice.2004

    • Author(s)
      Ogawa, R., Mizuno, H., Hyakusoku, H.Watanabe, A., Migita, M., Shimada, T.
    • Journal Title

      J.Nippon Med.Sch. 71

      Pages: 240-241

    • NAID

      130004146741

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Recipient non-hematopoietic bone marrow cells in the intestinal graft after fetal small intestinal transplantation.2004

    • Author(s)
      Kato, Y., Yamataka, A., Miyahara, K., Sueyoshi, N., Hayakawa, J., Hayashida, M., Migita, M., Shimada, T., Kobayashi, H., Lane, G.J., Miyano, T.
    • Journal Title

      Pediatr.Surg.Int. 20

      Pages: 1-4

    • NAID

      10018938349

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic and Chondrogenic Differentiation by Adipose-derived Stem Cells Harvested from GFP Transgenic Mice.2004

    • Author(s)
      Ogawa, R., Mizuno, H., et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 23

      Pages: 871-877

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Adeno-associated viral vector mediated expression on endostatin inhibits tumor growth and metastasis in an orthotropic pancreatic cancer model in hamsters.2004

    • Author(s)
      Noro, T., Miyake, K., et al.
    • Journal Title

      Cancer Res. 64

      Pages: 7486-7490

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Adipogenic defferentiation by adipose-derived stem cells harvested from GFP transgenic mice - including relationship of sex differences -.2004

    • Author(s)
      Ogawa, R., Mizuno, H., et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 319

      Pages: 511-517

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Chondrogenic and osteogenic differentiation of adipose-derived stem cells isolated from GFP transgenic mice.2004

    • Author(s)
      Ogawa, R., Mizuno, H., et al.
    • Journal Title

      J.Nippon Med.Sch. 71

      Pages: 240-241

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Glucocerebrosidase level in the cerebrospinal fluid during enzymereplacement therapy -unsuccessful treatment to the neurological abnormality of type 2 Gaucher disease.2003

    • Author(s)
      Migita M., et al.
    • Journal Title

      Eur. J. Paed. 162

      Pages: 524-525

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Lentivirus-mediated expression of angiostatin efficiently inhibits neovascularization in a murine proliferative retinopathy model.2003

    • Author(s)
      Igarashi, T., et al.
    • Journal Title

      Gene Ther. 10

      Pages: 219-226

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Migration of EGFP expressing bone marrow-derived microglia/macrophage into the mice brain following permanent focal ischemia.2003

    • Author(s)
      Tanaka, R., et al.
    • Journal Title

      Neuroscience 117

      Pages: 531-539

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Home treatment with enzyme replacement therapy for Gaucher disease.2003

    • Author(s)
      Migita, M., et al.
    • Journal Title

      Pediatr. Int. 45

      Pages: 363-365

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bone formation following transplantation of genetically modified primary bone marrow stromal cells.2003

    • Author(s)
      Sugiyama, O., et al.
    • Journal Title

      J. Orthop. Res. 21

      Pages: 630-637

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Establishment of modified chimeric mice using GFP bone marrow as a model for neurological disorders.2003

    • Author(s)
      Furuya, T., et al.
    • Journal Title

      NeuroReport 14

      Pages: 629-631

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Little evidence of transdifferentiation of bone marrow-derived cells into pancreatic beta-cells.2003

    • Author(s)
      Choi, J.B., et al.
    • Journal Title

      Diabetologia 46

      Pages: 1366-1374

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Generation of a chimeric mouse reconstituted with green fluorescent protein-positive bone marrow cells : a useful model for studying the behaviour of bone marrow cells in regeneration in vivo.2003

    • Author(s)
      Hayakawa, J., et al.
    • Journal Title

      Int. J. Hematol. 77

      Pages: 456-462

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cardiomyocyte regeneration from circulating bone marrow cells in mice.2003

    • Author(s)
      Kuramochi, Y., et al.
    • Journal Title

      Pediatr. Res. 77

      Pages: 456-462

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic and Chondrogenic Differentiation by Adipose-derived Stem Cells Harvested from GFP Transgenic Mice.2003

    • Author(s)
      Ogawa, R., et al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 54

      Pages: 319-325

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Glucocerebrosidase level in the cerebrospinal fluid during enzymereplacement therapy -unsuccessful treatment to the neurological abnormality of type 2 Gaucher disease.2003

    • Author(s)
      Migita M., Hamada, H., Fujimura, J., Watanabe, A., Shimada, T., Fukunaga, Y.
    • Journal Title

      Eur.J.Paed. 162

      Pages: 524-525

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Lentivirus-mediated expression of angiostatin efficiently inhibits neovascularization in a murine proliferative retinopathy model.2003

    • Author(s)
      Igarashi, T., Miyake, K., Kato, K., Watanabe, A., Ishizaki, M., Ohara, K., Shimada, T.
    • Journal Title

      Gene Ther. 10

      Pages: 219-226

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Migration of EGFP expressing bone marrow-derived microglia/macrophage into the mice brain following permanent focal ischemia.2003

    • Author(s)
      Tanaka, R., Komine-Kobayashi, M., Mochizuki, H., Yamada, M., Furuya, T., Migita, M., Shimada, T., Mizuno, Y., Urabe, T.
    • Journal Title

      Neuroscience 117

      Pages: 531-539

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Home treatment with enzyme replacement therapy for Gaucher disease.2003

    • Author(s)
      Migita, M., Shimada, T., Hayakawa, J., Morita, T., Fukunaga, Y.
    • Journal Title

      Pediatr.Int. 45

      Pages: 363-365

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bone formation following transplantation of genetically modified primary bone marrow stromal cells.2003

    • Author(s)
      Sugiyama, O., Orimo, H., Suzuki, S., Yamashita, K., Ito, H., Shimada, T.
    • Journal Title

      J.Orthop.Res. 21

      Pages: 630-637

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Establishment of modified chimeric mice using GFP bone marrow as a model for neurological disorders.2003

    • Author(s)
      Furuya, T., Tanaka, R., Urabe, T., Hayakawa, J., Migita, M., Shimada, T., Mizuno, Y., Mochizuki, H.
    • Journal Title

      NeuroReport 14

      Pages: 629-631

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Little evidence of transdifferentiation of bone marrow-derived cells into pancreatic beta-cells.2003

    • Author(s)
      Choi, J.B., Uchino, H., Azuma, K., Iwashita, N., Tanaka, Y., Mochizuki, H., Migita, M., Shimada, T., Kawamori, R., Watada, H.
    • Journal Title

      Diabetologia 46

      Pages: 1366-1374

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Generation of a chimeric mouse reconstituted with green fluorescent protein-positive bone marrow cells : a useful model for studying the behavior of bone marrow cells in regeneration in vivo.2003

    • Author(s)
      Hayakawa, J., Migita, M., Ueda, T., Shimada, T., Fukunaga, Y.
    • Journal Title

      Int.J.Hematol. 77

      Pages: 456-462

    • NAID

      50000861650

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cardiomyocyte regeneration from circulating bone marrow cells in mice.2003

    • Author(s)
      Kuramochi, Y., Fukazawa, R., Migita, M., Hayakawa, J., Hayashida, M., Uchikoba, Y., Fukumi, D., Shimada, T., Ogawa, S.
    • Journal Title

      Pediatr.Res. 54

      Pages: 319-325

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Glucocerebrosidase level in the cerebrospinal fluid during enzymereplacement therapy - unsuccessful treatment to the neurological abnormality of type 2 Gaucher disease.2003

    • Author(s)
      Migita M., Hamada, H., et al.
    • Journal Title

      Eur.J.Paed. 162

      Pages: 524-525

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Lentivirus-mediated expression of angiostatin efficiently inhibits neovascularization in a murine proliferative retinopathy model.2003

    • Author(s)
      Igarashi, T., Miyake, K., et al.
    • Journal Title

      Gene Ther. 10

      Pages: 219-226

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Migration of EGFP expressing bone marrow-derived microglia/macrophage into the mice brain following permanent focal ischemia.2003

    • Author(s)
      Tanaka, R., Komine-Kobayashi, M., et al.
    • Journal Title

      Neuroscience 117

      Pages: 531-539

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Home treatment with enzyme replacement therapy for Gaucher disease.2003

    • Author(s)
      Migita, M., Shimada, T., et al.
    • Journal Title

      Pediatr.Int. 45

      Pages: 363-365

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Bone formation following transplantation of genetically modified primary bone marrow stromal cells.2003

    • Author(s)
      Sugiyama, O., Orimo, H., et al.
    • Journal Title

      J.Orthop.Res. 21

      Pages: 630-637

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Establishment of modified chimeric mice using GFP bone marrow as a model for neurological disorders.2003

    • Author(s)
      Furuya, T., Tanaka, R., et al.
    • Journal Title

      NeuroReport 14

      Pages: 629-631

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Little evidence of transdifferentiation of bone marrow-derived cells into pancreatic beta-cells.2003

    • Author(s)
      Choi, J.B., Uchino, H., et al.
    • Journal Title

      Diabetologia 46

      Pages: 1366-1374

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Generation of a chimeric mouse reconstituted with green fluorescent protein-positive bone marrow cells: a useful model for studying the behaviour of bone marrow cells in regeneration in vivo.2003

    • Author(s)
      Hayakawa, J., Migita, M., et al.
    • Journal Title

      Int.J.Hematol. 77

      Pages: 456-462

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cardiomyocyte regeneration from circulating bone marrow cells in mice.2003

    • Author(s)
      Kuramochi, Y., Fukazawa, R., et al.
    • Journal Title

      Pediatr.Res. 54

      Pages: 319-325

    • Related Report
      2004 Annual Research Report
  • [Journal Article] New strategy for in vivo transgene expression in corneal epithelial progenitor cells.2002

    • Author(s)
      Igarashi T., et al.
    • Journal Title

      Current Eye Res. 24

      Pages: 46-50

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Long-term systemic therapy of Fabry disease in a knockout mouse by adeno-associated virus-mediated muscle-directed gene transfer.2002

    • Author(s)
      Takahashi, H., et al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA. 99

      Pages: 13777-13782

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Adeno-associated virus mediated antiapoptotic gene delivery : in vivo gene therapy for neurological disorders.2002

    • Author(s)
      Mochizuki, H., et al.
    • Journal Title

      Methods 28

      Pages: 248-252

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] New strategy for in vivo transgene expression in corneal epithelial progenitor cells.2002

    • Author(s)
      Igarashi, T., Miyake, K., Suzuki, N., Kato, K., Takahashi, H., Ohara, K., Shimada, T.
    • Journal Title

      Current Eye Res. 24

      Pages: 46-50

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Long-term systemic therapy of Fabry disease in a knockout mouse by adeno-associated virus-mediated muscle-directed gene transfer.2002

    • Author(s)
      Takahashi, H., Hirai, Y., Migita, M., Seino, Y., Fukuda, Y., Sakuraba, H., Kase, R., Kobayashi, T., Hashimoto, Y., Shimada, T.
    • Journal Title

      Proc.Natl.Acad.Sci.USA. 99

      Pages: 13777-13782

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Adeno-associated virus mediated antiapoptotic gene delivery : in vivo gene therapy for neurological disorders.2002

    • Author(s)
      Mochizuki, H., Miura, M., Shimada, T., Mizuno, Y.
    • Journal Title

      Methods 28

      Pages: 248-252

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] New strategy for in vivo transgene expression in corneal epithelial progenitor cells.2002

    • Author(s)
      Igarashi, T., Miyake, K., et al.
    • Journal Title

      Current Eye Res. 24

      Pages: 46-50

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Long-term systemic therapy of Fabry disease in a knockout mouse by adeno-associated virus-mediated muscle-directed gene transfer.2002

    • Author(s)
      Takahashi, H., Hirai, Y., et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA. 99

      Pages: 13777-13782

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Anti-apoptotic therapy for Parkinson's disease: overexpression of an apf-1-dominant-gnegative inhibitor can block MPTP toxicity. Mapping the Program of Alzheimer's and Parkinson's Disease. (edited by Mizuno et al.)2002

    • Author(s)
      Mochizuki, H., Hayakawa, H., et al.
    • Journal Title

      Kluwer Academic Publishers

      Pages: 469-472

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Adeno-associated virus mediated antiapoptotic gene delivery; in vivo gene therapy for neurological disorders.2002

    • Author(s)
      Mochizuki, H., Miura, M., et al.
    • Journal Title

      Methods 28

      Pages: 248-252

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  • [Book] Anti-apoptotic therapy for Parkinson's disease : overexpression of an apf-1-dominant-gnegative inhibitor can block MPTP toxicity. Mapping the Program of Alzheimer's and Parkinson's Disease. (edited by Mizuno et al.)2002

    • Author(s)
      Mochizuki, H., Hayakawa, H., Nigita, M., Shimada, T., Masyuki, M., Mizuno, Y.
    • Publisher
      Kluwer Academic Publishers
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 脂肪組織由来幹細胞からの骨髄を形成するための組成物およぴその方法2005

    • Inventor(s)
      小川令, 水野博司, 百束比古, 島田隆
    • Industrial Property Rights Holder
      小川令, 水野博司, 百束比古, 島田隆
    • Industrial Property Number
      2004-133827
    • Filing Date
      2005-04-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Igarashi, T., et al.: "Lentivirus-mediated expression of angiostatin efficiently inhibits neovascularization in a murine proliferative retinopathy model."Gene Ther.. 10. 219-226 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka, R., et al.: "Migration of EGFP expressing bone marrow-derived microglia/macrophage into the mice brain following permanent focal ischemia."Neuroscience. 117. 531-539 (2003)

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      2003 Annual Research Report
  • [Publications] Sugiyama, O., et al.: "Bone formation following transplantation of genetically modified primary bone marrow stromal cells."J.Orthop.Res.. 21. 630-637 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Furuya, T., et al.: "Establishment of modified chimeric mice using GFP bone marrow as a model for neurological disorders."NeuroReport. 14. 629-631 (2003)

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      2003 Annual Research Report
  • [Publications] Choi, J.B., et al.: "Little evidence of transdifferentiation of bone marrow-derived cells into pancreatic beta-cells."Diabetologia. 46. 1366-1374 (2003)

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      2003 Annual Research Report
  • [Publications] Ogawa, R., et al.: "Osteogenic and Chondrogenic Differentiation by Adipose-derived Stem Cells Harvested from GFP Transgenic Mice."Biochem.Biophys.Res.Commun..

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      2003 Annual Research Report
  • [Publications] Inokuchi, K., Yamaguchi, H., Hanawa, H., Tanosaki, et al.: "Loss of DCC gene expression is of prognostic importance in acute myelogenous leukemia"Clinical Cancer Res.. 8. 1882-1888 (2002)

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      2002 Annual Research Report
  • [Publications] Igarashi, T., Miyake, K., Kato, K., Watanabe, A., et al.: "Lentivirus-mediated expression of angiostatin efficiently inhibits neovascularization in a murine proliferative retinopathy model"Gene Ther.. 10. 219-226 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Igarashi, T., Miyake, K., Suzuki, N., Kato, K., et al.: "New strategy for in vivo transgene expression in corneal epithelial progenitor cells"Current Eye Res.. 24. 46-50 (2002)

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      2002 Annual Research Report
  • [Publications] Takahashi, H., Hirai, Y., Migita, M., Seino, Y., et al.: "Long-term systemic therapy of Fabry disease in a knockout mouse by adeno-associated virus-mediated muscle-directed gene transfer"Proc. Natl. Acad. Sci. USA.. 99. 13777-13782 (2002)

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  • [Publications] Tanaka, R., Komine-Kobayashi, M., Mochizuki, H., Yamada, et al.: "Migration of EGFP expressing bone marrow-derived microglia/macrophage into the mice brain following permanent focal ischemia"Neuroscience. (in press). (2003)

    • Related Report
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  • [Publications] Sugiyama, O., Orimo, H., Suzuki, S., Yamashita, K., et al.: "Bone formation following transplantation of genetically modified primary bone marrow stromal cells"J. Orthop. Res.. (in press). (2003)

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      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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