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Kinetic propertie of three Darier disease SERCA26 mutants.

Research Project

Project/Area Number 14370256
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionAsahikawa Medical College

Principal Investigator

IIZUKA Hajime  Asahikawa Medical College, Department of Dermatology, Professor, 医学部, 教授 (90113513)

Co-Investigator(Kenkyū-buntansha) ISHIDA Akemi (YAMAMOTO Akemi)  Asahikawa Medical College, Department of Dermatology, Instructor, 医学部, 講師 (30241441)
TAKAHASHI Hidetoshi  Asahikawa Medical College, Department of Dermatology, Instructor, 医学部, 講師 (00216748)
NAKAMURA Satoshi  Asahikawa Medical College, Department of Dermatology, Assistant, 医学部, 助手 (20292112)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2002: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsDarier disease / endoplasmic reticulum / calcium pump / keratinization
Research Abstract

The functional abnormalities in Darier disease-causing calcium ATPase (Serca2B) mutants were explored. Among 51mutants, 15 showed negligible expression of SERCA2b, 21 showed negative ATPase activities, and 8 showed functional uncoupling of ATPase and calcium pump activities in Cos1 cells. The remaining 3 showed unique characteristics. They exhibited nearly normal expression, high ATPase and coupled calcium transport activities that were essentially identical (L321F) or slightly lower (I274V and M719I) as compared with those of the wild type. M719I and L321F possess 2-3 fold reduced affinity for cytoplasmic calcium, whereas I274V possesses the normal high affinity. L321F exhibited also the remarkably reduced sensitivity to the feedback inhibition of the transport cycle by accumulated luminal calcium. Thus different abnormalities in Darier disease causing ATPase (SERCA2b) showed distinct types of altered calcium homeostasis in Cos1 cells.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (28 results)

All 2005 2004 2003 Other

All Journal Article (23 results) Publications (5 results)

  • [Journal Article] Prediction of a coding sequence for a novel type II keratin2005

    • Author(s)
      Senshu T, Iizuka H, et al.
    • Journal Title

      Journal of Dermatological Science 37

      Pages: 41-48

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] LEKTI is localized in lamellar granules2005

    • Author(s)
      Ishida-Yamamoto A, Iizuka H, et al.
    • Journal Title

      Journal of Investigative Dermatology 124

      Pages: 360-366

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epidermal lamellar granules transport different cargoes2005

    • Author(s)
      Ishida-Yamamoto A, Iizuka H, et al.
    • Journal Title

      Journal of Investigative Dermatology 122

      Pages: 1137-1144

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] LEKTI is localized in lamellar granules, separated from KLK5 and KLK7, and is secreted in the extracellular spaces of the superficial stratum granulosum.2005

    • Author(s)
      Ishida-Yamamoto A, Deraison C, Bonnart C, Bitoun E, Robinson R, O'Brien TJ, Wakamatsu K, Ohtsubo S, Takahashi H, Hashinoto Y, Dopping-Hepenstal PJ, McGrath JA, Iizuka H, Richard G, Hovnanian A.
    • Journal Title

      J Invest Dermatol 124

      Pages: 360-366

    • NAID

      120003161707

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prediction of a coding sequence for a novel type II keratin from N-terminal sequences of mouse epidermal proteins site-specifically deiminated in embryonic development.2005

    • Author(s)
      Senshu T, Ishida-Yamamoto A, Takahashi H, Iizuka H.
    • Journal Title

      J Dermatol Sci 37(1)

      Pages: 41-48

    • NAID

      10018030387

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] LEKTI Is Localized in Lamellar Granules, Separated from KLK5 and KLK7, and Is Secreted in the Extracellular Spaces of the Superficial Stratum Granulosum2005

    • Author(s)
      Ishida-Yamamoto A, Deraison C, Iizuka H, et al.
    • Journal Title

      Journal of Investigative Dermatology 124

      Pages: 360-366

    • NAID

      120003161707

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Prediction of a coding sequence for a novel type II keratin from N-terminal sequences of mouse epidermal proteins site-specifically deminated in embryonic development2005

    • Author(s)
      Senshu T, Iizuka H, et al.
    • Journal Title

      Journal of Dermatological Science 37

      Pages: 41-48

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Distinct types of abnormality in kinetic properties of three Darier disease2004

    • Author(s)
      Sato K, Yamasaki K, Iizuka H, et al.
    • Journal Title

      Journal of Biological Chemistry 279

      Pages: 35595-35603

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Psoriatic architecture constructed by epidermal remodeling2004

    • Author(s)
      Iizuka H, Takahashi H, Ishida-Yamamoto A
    • Journal Title

      Journal of Dermatological Science 35

      Pages: 93-99

    • NAID

      10016184480

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Unique fertilization process in psoriasis2004

    • Author(s)
      Iizuka H, Takahashi H, Honma N, Ishida-Yamamoto A
    • Journal Title

      Journal of Dermatology 31

      Pages: 271-276

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of bepotastine, cetirizine, fexofenadine, and olopatadine on histamine-induced wheal-and flare-response, sedation, and psychomotor performance.2004

    • Author(s)
      Takahashi H, Ishida-Yamamoto A, Iizuka H.
    • Journal Title

      Clin Exp Dermatol 29

      Pages: 526-532

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cyclic AMP differentially regulates cell proliferation of normal human keratinocytes through ERK activation depending on the expression pattern of B-Raf.2004

    • Author(s)
      Takahashi H, Honma M, Miyauchi Y, Nakamura S, Ishida-Yamamoto A, Iizuka H.
    • Journal Title

      Arch Dermatol Res 296

      Pages: 74-82

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Psoriatic architecture constructed by epidermal remodeling.2004

    • Author(s)
      Iizuka H, Takahashi H, Ishida-Yamamoto A.
    • Journal Title

      J Dermatol Sci 35

      Pages: 93-99

    • NAID

      10016184480

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distinct types of abnormality in kinetic properties of three Darier disease-causing sarco(endo)plasmic reticulum Ca2+-ATPase mutants that exhibit normal expression and high Ca2+ transport activity.2004

    • Author(s)
      Sato K, Yamasaki K, Daiho T, Miyauchi Y, Takahashi H, Ishida-Yamamoto A, Nakamura S, Iizuka H, Suzuki H.
    • Journal Title

      J Biol Chem 279

      Pages: 35595-35603

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Unique keratinization process in psoriasis : late differentiation markers are abolished because of the premature cell death.2004

    • Author(s)
      Iizuka H, Takahashi H, Honma M, Ishida-Yamamoto A.
    • Journal Title

      J Dermatol 31

      Pages: 271-276

    • NAID

      10016129386

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epidermal lamellar granules transport different cargoes as distinct aggregates.2004

    • Author(s)
      Ishida-Yamamoto A, Simon M, Kishibe M, Miyauchi Y, Takahashi H, Yoshida S, O'Brien TJ, Serre G, Iizuka H.
    • Journal Title

      J Invest Dermatol 122

      Pages: 1137-1144

    • NAID

      120003161704

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distinct types of abnormality in kinetic properties of three Darier disease-causing Sarco(endo)plasmic Reticulum Ca^<2+>-ATPase Mutants That Exhibit Normal Expression and High Ca^<2+> Transport Activity2004

    • Author(s)
      Sato K, Yamasaki K, Iizuka H, et al.
    • Journal Title

      Journal of Biological Chemistry 279

      Pages: 35595-35603

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Unique keratinization process in psoriasts : late differentiation markers are abolished2004

    • Author(s)
      Iizuka H, Takahashi H, Honma M, Ishida-Yamamoto A
    • Journal Title

      Journal of Dermatology 31

      Pages: 271-276

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Epidermal lamellar granules transport different cargos as distinct aggregates2004

    • Author(s)
      Ishida-Yamamoto A, Simon M, Iezuka H et al.
    • Journal Title

      Journal of Investigative Dermatology 122

      Pages: 1137-1144

    • NAID

      120003161704

    • Related Report
      2004 Annual Research Report
  • [Journal Article] SLURP-2, a novel member of the human Ly-6 superfamily that is uregulated in psoriasis vulgaris.2003

    • Author(s)
      Tsuji h, Okamoto K, Matsuzaka Y, Iizuka H, Tamiya G, Inoko H
    • Journal Title

      Genomics 81

      Pages: 26-33

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Activation of two caspase cascades, caspase 8/3/6 and caspase 9/3/6, during photodynamic therapy using a novel photosensitizer, ATX-S10(Na), in normal human keratinocytes.2003

    • Author(s)
      Takahashi H, Itoh Y, Miyauchi Y, Nakajima S, Sakata I, Ishida-Yamamoto A, Iizuka H.
    • Journal Title

      Arch Dermatol Res 295

      Pages: 242-248

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 1,25-dihydroxyvitamin D(3) increases human cystatin A expression by inhibiting the Raf-1/MEK1/ERK signaling pathway of keratinocytes.2003

    • Author(s)
      Takahashi H, Ibe M, Honma M, Ishida-Yamamoto A, Hashinoto Y, Iizuka H.
    • Journal Title

      Arch Dermatol Res 295

      Pages: 80-87

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Discoid lupus erythematosus exacerbated by radiation therapy.

    • Author(s)
      Ishida-Yamamoto A, Kanno K, Sato E, Takahashi H, Iizuka H.
    • Journal Title

      J Dermatol (in press)

    • NAID

      10020272881

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Daiho T, Yamasaki K, Iizuka H et al.: "Deletion of any single residues in Glu^<40>-Ser^<48> loop connecting a domain and the first transmembrane helix of sarcoplasmic reticulum Ca^<2+>-ATPase result in almost complete inhibition of conformational transition and hydrolysis of phosphoenzyme intermediate"Journal of Biological Chemistry. 278. 39197-39102 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahashi H, Ibe M, Ishida-Yamamoto A, Iizuka H et al.: "1,25-Dihydroxyvitamin D3 increases human cystatin A expression by inhibiting the Raf-1/MEK1/ERK signaling pathway of keratinocytes"Archives of Dermatological Research. 295. 80-87 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ishida-Yamamoto A, Gabriele Richard, Takahashi H, Iizuka H: "In vivo studies of mutant K1 in Ichthyosis Hystrix Curth-Macklin"Journal of Investigative Dermatology. 120. 498-500 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsuji H, Okamoto K, Iizuka H et al.: "SLURP-2, a novel member of the human Ly-6 superfamily that is upregulated in psoriasis vulgaris"Genomics. 81. 26-33 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Wada T, Shirakata Y, Takahashi H, Iizuka H et al.: "A Japanese case of Darier's disease caused by mosaicism for the ATP2A2 mutation"British Journal of Dermatol. 149. 185-188 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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