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Experimental study for the recovery from the radiation induced intestinall damage by transplantation of ES cells and stimulation of cytokines in mice.

Research Project

Project/Area Number 14370267
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionHirosaki University

Principal Investigator

ABE Yoshinao  Hirosaki University, School of Medicine, Professor, 医学部, 教授 (10167950)

Co-Investigator(Kenkyū-buntansha) AOKI Masahiko  Hirosaki University, University Hospital, Lecture, 医学部附属病院, 講師 (70292141)
KASHIWAKURA Ikuo  Hirosaki University, School of Medicine, Professor, 医学部, 教授 (00177370)
NAKANE Akio  Hirosaki University, School of Medicine, Professor, 医学部, 教授 (30164239)
KUDO Kohsei  Hirosaki University, School of Medicine, Assistant, 医学部, 助手 (10214967)
近藤 英宏  弘前大学, 医学部附属病院, 助手 (70333721)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥8,100,000 (Direct Cost: ¥8,100,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2002: ¥4,000,000 (Direct Cost: ¥4,000,000)
Keywordsmouse ES cell / Radiation damage / Regenerative Medicine / growth stimulating factor / free radical scavenger / intestinal colony / 放射線腸障害
Research Abstract

The radiation induced intestinal damage is fatal because of the impaired regeneration of intestinal crypts. We studied two possible ways of the recovery from the intestinal damage ; 1)transplantation of ES cells directly to the damaged intestine and 2)assistance of the regeneration capacity by stimulation of cytokines and other substances ; such as epidermal growth factor (EGF), melatonin and hydroxyl radical scavenger-edaravone.
1)Transplantation of ES cells to the heavily irradiated area of the intestine
After a single dose of 30Gy to a small part of intestine, ES cells were injected directly to the irradiated part. An appropriated days after transplantation, macroscopic colony was found surrounded by the denuded area of the intestine. Microscopically, colony was consisted of undifferentiated and differentiated cells constructing the acinar structures like crypt. PCR revealed that the colony cells were derived from ES cells. Colony are being stained for several special markers using immunohistochemistry. It is concluded that ES cells can proliferate and differentiated in the irradiated intestine. We have not yet presented these results and we are now preparing the results for submission to a few papers.
2)The effect of EGF, melatonin and edaravone
EGF and melatonin were administered after a total body irradiation to the mouse fatally. They bad no effect on the colony formation of the crypt. However, edaravone administered 30 to 60 minutes before irradiation, the number of colony was increased. Edaravone had a potential to scavenge free hydroxyl radicals derived from radiation in vivo. A part of the paper was presented and published in Japanese.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (49 results)

All 2005 2004 2003 2002 Other

All Journal Article (34 results) Book (2 results) Publications (13 results)

  • [Journal Article] Formulation of spectra-based attenuation coefficients in water as a function of depth and off-axis distance for 4, 10, and 15 MV X-ray beams.2005

    • Author(s)
      A Iwasaki
    • Journal Title

      Radiat Phys Chem 72

      Pages: 657-661

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Impairment of host resistance to bisteria monocytogenes infection in liver of db/db and oh/oh mice.2005

    • Author(s)
      S.Ikejima
    • Journal Title

      Diabetes 54

      Pages: 182-189

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A method for evaluating the degree of random-periodicity of radiographic noise containing periodic siganals.2005

    • Author(s)
      A.Iwasaki
    • Journal Title

      Bull.Health Sci.Hirosaki 4

      Pages: 101-107

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] マウスX線全身照射による放射線傷害に対するEGF、メラトニン、エダラボンの影響2005

    • Author(s)
      工藤幸清
    • Journal Title

      弘前大学医学部保健学科紀要 4

      Pages: 109-113

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 直腸部X線照射によるマウス直腸障害モデルの作成2005

    • Author(s)
      篠崎信世
    • Journal Title

      弘前大学医学部保健学科紀要 4

      Pages: 115-122

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Impairment of host resistance to Listeria monocytogenes infection in liver of ob/ob and ob/ob mice.2005

    • Author(s)
      S.Ikejima
    • Journal Title

      Diabetes 54

      Pages: 182-189

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Impairment of host resistance to Listeria monocytogenes infection in liver of db/db and ob/ob mice.2005

    • Author(s)
      S.Ikejima
    • Journal Title

      Diabetes 54

      Pages: 182-186

    • Related Report
      2004 Annual Research Report
  • [Journal Article] マウスX線全身照射による放射線傷害に対するEGF、メラトニン、エダラボンの影響2005

    • Author(s)
      工藤 幸清
    • Journal Title

      弘前大学医学部保健学科紀要 4

      Pages: 109-113

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 直腸部X腺照射によるマウス直腸障害モデル作製2005

    • Author(s)
      篠崎 信世
    • Journal Title

      弘前大学医学部保健学科紀要 4

      Pages: 115-122

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A method for evaluating the degree of random-periodicity of radiographic noise containing periodic signals2005

    • Author(s)
      A.Iwasaki
    • Journal Title

      Bull.Health Sci.Hirosaki 4

      Pages: 101-107

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Theoretical considerations with the Wiener spectrum used for evaluation of radiographic granularity.2004

    • Author(s)
      A.Iwasaki
    • Journal Title

      Bull.Health Sci.Hirosaki 3

      Pages: 25-32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Regenerative induction of megakaryopoiesis and thrombopoiesis in vitro from X-irradiated CD34+ cells prepared from human placental and umbilical cord blood.2004

    • Author(s)
      I.Kashiwakura
    • Journal Title

      Bull.Health Sci.Hirosaki 3

      Pages: 17-24

    • NAID

      110004772466

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Weekly paclitaxerl and nedaplatin with concurrent radiotherapy for locally advanced non-small-cell lung cancer : a phase I/II study.2004

    • Author(s)
      Y.Hasegawa
    • Journal Title

      Jpn J Clin Oncol 34

      Pages: 647-653

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 心臓カテーテル検査施行医師の被ばく線量の検討2004

    • Author(s)
      金 正宣
    • Journal Title

      心臓 36

      Pages: 667-672

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 放射線治療における非癌部の被ばくと防護2004

    • Author(s)
      阿部由直
    • Journal Title

      Radiology Frontier 7

      Pages: 289-293

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Theoretical considerations with the Wiener spectrum used for evaluation of radiographic granularity.2004

    • Author(s)
      A.Iwasaki
    • Journal Title

      Bull Health Sci Hirosaki 3

      Pages: 25-32

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Regenerative induction of megakaryopoiesis and thrombopoiesis in vitro from X-irradiated CD34+ cells prepared from human placental and umbilical cord blood.2004

    • Author(s)
      I.Kashiwakura
    • Journal Title

      Bull Health Sci Hirosaki 3

      Pages: 17-24

    • NAID

      110004772466

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Weekly pachtaxrl and nedaplatin with concurrent radiotheerapy for locally advanced non-small-cell lung cancer : a phase I/II study.2004

    • Author(s)
      Y.Hasegawa
    • Journal Title

      Jpn J Clin Oncol 34

      Pages: 647-653

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A practical method for estimating high-energy X-ray spectra using the iterative perturbation principle proposed by Waggener.2003

    • Author(s)
      A.Iwasaki
    • Journal Title

      Radiat Phys Chem 67

      Pages: 81-91

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A method for obtaining 4 and 10 MV X-rays spectra using iterative perturbation principle.2003

    • Author(s)
      A.Iwasaki
    • Journal Title

      Bull.Health Sci.Hirosaki 2

      Pages: 61-72

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 定位的放射線治療(SRT)による聴神経腫瘍の治療成績2003

    • Author(s)
      真里谷靖
    • Journal Title

      弘前医学 55

      Pages: 7-17

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 重粒子線治療の生物学2003

    • Author(s)
      阿部由直
    • Journal Title

      癌の臨床 49

      Pages: 1541-1543

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A method for obtaining 4 and 10 MV X-rays spectra using iterative perturbation principle.2003

    • Author(s)
      A.Iwasaki
    • Journal Title

      Bull.Health Sci Hirosaki 2

      Pages: 61-72

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Calculation of 10MV X-ray beam-axis doses in layered cork heterogeneity phantoms using a convolution method.2002

    • Author(s)
      A.Iwasaki
    • Journal Title

      Bull Health Sci Hirosaki 1

      Pages: 61-82

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ラット実験腫瘍におけるインドシアニングリーンと近赤外光トポグラフィーを用いた癌診断法の基礎的研究2002

    • Author(s)
      大畑 崇
    • Journal Title

      日本医学放射線学会雑誌 62

      Pages: 284-286

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 癌治療とアポトーシス(古典的観点から)2002

    • Author(s)
      阿部由直
    • Journal Title

      癌の臨床 48

      Pages: 349-351

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinical outcome of hypofractionated conventional conformation radiotherapy for patients with single and no more than three metastatic brain tumors using noninvasive fixation of the skull without whole brain irradiation.

    • Author(s)
      M.Aoki
    • Journal Title

      Int J Radiat Oncol Biol Phys (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Surface appearance of the vertebrobasilar artery revealed on basiparallel anatomical scanning (BPAS)- MRI : Its role for the brain MR examination.

    • Author(s)
      M.Nagahata
    • Journal Title

      AJNR (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Different Radiosensitive Megakaryocytic Progenitor Cells Are Existed In Normal Human Peripheral Blood.

    • Author(s)
      I.Kashiwakura
    • Journal Title

      Radiat Res (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinical outcome of hypofractionated conventional conformation radiotherapy for patients with single and no more than three metastatic brain tumors using noninvasive fixation of the skull without whole brain irradiation.

    • Author(s)
      M.Aoki
    • Journal Title

      Int J Radiat Oncol Biol Phys (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Surface appearance of the vertebrobasilar artery revealed on bast-parallel anatomical scanning (BPAS)-MRI : Its role for the brain MR examination.

    • Author(s)
      M.Nagahata
    • Journal Title

      AJNR (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Different radiosensitive megakaryocytic progenitor cells are existed in normal human peripheral blood.

    • Author(s)
      I.Kashiwakura
    • Journal Title

      Radiat Res (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Different Radiosensitive Megakaryocytic Progenitor Cells Are Existed In Normal Human Peripheral Blood.

    • Author(s)
      I.Kashiwakura
    • Journal Title

      Radiat Res (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Formulation of spectra-based attenuation coefficients in water as a function of depth and off-axis distance for 4, 10, and 15 MV X-ray beams.

    • Author(s)
      A.Iwasaki
    • Journal Title

      Radiat Phys Chem (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Book] 放射線肺炎と放射線肺線維症(藤村重文編)2004

    • Author(s)
      阿部由直
    • Publisher
      金原出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 正常組織反応(大川、田中、佐々木編)2002

    • Author(s)
      阿部由直
    • Publisher
      篠原出版新社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] A.Iwasaki: "Theoretical considerations with the Wiener spectrum used for evaluation of radiographic granularity"Bull Health Sci Hirosaki. 3. 25-32 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Iwasaki: "A practical method for estimating high-energy X-ray spectra using the iterative perturbation principle proposed by Waggener"Radiat Phys Chem. 67. 81-91 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Iwasaki: "A method for obtaining 4 and 10 MV X-rays spectra using iterative perturbation principle"Bull Health Sci Hirosaki. 2. 61-72 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 阿部 由直: "重粒子線の生物学"癌の臨床. 49. 1541-1543 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] I.Kashiwakura: "Protective effects of thrombopoietin and stem cell factor on X-irradiated CD34 megakaryocytic progenitor cells from human placental and umbilical cord blood"Radiat Res. 160. 210-216 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hu, D.-L.: "Vaccination with nontoxic mutant toxic shock syndrome toxin 1 protects against Staphylococcus aureus infection"J Infect Dis. 188. 743-752 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 阿部 由直: "第5回放射線腫瘍学夏期セミナー要旨集"正常組織障害における放射線生物学と臨床の接点. 5 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Iwasaki: "Practical method for estimating high-energy X-ray spectra using the iterative perturbation principle proposed by Waggener"Radiat Phys Chem. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] A.Iwasaki: "A method for obtaining 4 and 10 MV X-rays spectra using iterative perturbation principle"Bull. Health Sci. Hirosaki. 2. 61-72 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] A.Iwasaki: "Calculation for 10MV X-ray beam-axis doses in layered cork heterogeneity phantoms using a convolution method"Bull. Health Sci. Hirosaki. 1. 61-82 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大畑 崇: "ラット実験腫瘍におけるインドシアニングリーンと近赤外光トポグラフィーを用いた癌診断法の基礎的研究"日本医学放射線学会誌. 62. 284-286 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 阿部由直: "癌治療とアポトーシス(古典的観点から)"癌の臨床. 48. 349-351 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 阿部由直(分担執筆): "正常組織障害"癌・放射線療法2002 篠原出版新社 大川, 田中, 佐々木編. 135-146 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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