| Project/Area Number |
14370340
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Nagoya City University |
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Principal Investigator |
YOKOYAMA Shinji Nagoya City University, Graduate School of Medical Sciences, Professor, 大学院・医学研究科, 教授 (10142192)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJITA Maki Nagoya City University, Graduate School of Medical Sciences, Research Associate, 大学院・医学研究科, 助手 (10253262)
ABE Sumiko (DOHMAE Sumiko) Nagoya City University, Graduate School of Medical Sciences, Assistant Professor, 大学院・医学研究科, 講師 (70227700)
ITO Jinichi Nagoya City University, Graduate School of Medical Sciences, Associate Professor, 大学院・医学研究科, 助教授 (60167260)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2003: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2002: ¥8,600,000 (Direct Cost: ¥8,600,000)
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| Keywords | Cholesterol / HDL / ABCA1 / Apolipoprotein / Atherosclerosis / Brain / Astrocyte / アポリポ蛋白質 / アポリポタンパク質 |
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| Research Abstract |
1)ABCA1 is phosphorylated by RKCa activated by diacylglycerol generated in the reactions to replenish sphingomyelin that is removed by apoA-I to generate HDL. This reaction is generally observed with helical apolipoproteins that are capable of generating HDL, as well as the model peptides mimicking amphiphilic helices of apolipoprotein. 2)Human ABCA7 that is highly homologous to ABCA1mediates generation of HDL is stabilized by apolipoproteins in a similar manner to that of ABCA1 when expressed in HEK293. 3)Release of cellular cholesterol to HDL is mediated by two distinct mechanism ; non-specific exchange and an apolipoprotein/ABCA1 mediated pathway. The latter reaction was investigated by using an anti-apoA-I monoclonal antibody that recognizes only lipid free apoA-I. This antibody inhibited the ABCA1-dependent part of the cholesterol release reaction. The ABCA1-dependent HDL abiding to cells is only its protein moiety but not the lipid part. Thus, apoA-I dissociates from HDL and interacts with the cells to generate new HDL.
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